TOP2B 抗体
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北京 101111
Quick Overview for TOP2B 抗体 (ABIN271965)
抗原
See all TOP2B 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of Topoisomerase II β protein. (region surrounding Ala32)
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交叉反应 （详细）
- Species reactivity (tested):Human, Mouse.
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纯化方法
- Affinity Chromatography using epitope-specific immunogen.
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anti-Topoisomerase (DNA) II beta 180kDa (TOP2B) (AA 446-655) antibody
TOP2B 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) II beta 180kDa (TOP2B) (N-Term) antibodyTOP2B 适用: 人, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) II beta 180kDa (TOP2B) (C-Term) antibodyTOP2B 适用: 人, 小鼠 WB, IP, IHC (fp) 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) II beta 180kDa (TOP2B) (C-Term) antibodyTOP2B 适用: 人 WB, IF, IP, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) II beta 180kDa (TOP2B) (AA 1400-1626) antibodyTOP2B 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) II beta 180kDa (TOP2B) (AA 1475-1525) antibodyTOP2B 适用: 人 WB, IP, IHC (fp) 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) II beta 180kDa (TOP2B) (N-Term) antibodyTOP2B 适用: 人, 小鼠, 犬, 猴, Pig WB, IHC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) II beta 180kDa (TOP2B) (AA 1411-1523) antibodyTOP2B 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Topoisomerase (DNA) II beta 180kDa (TOP2B) (C-Term) antibodyTOP2B 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) II beta 180kDa (TOP2B) (AA 1439-1488) antibodyTOP2B 适用: 人, 小鼠, 大鼠, 犬, 猴, Pig, 豚鼠, 马, 兔, Bat WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))
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别名
- TOP2B
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背景
- Topoisomerases are nuclear enzymes involved in a variety of cellular activities such as chromosome condensation, DNA replication, transcription, recombination and segregation at mitosis. Human topoisomerase I is a 100kD protein capable of relaxing positively and negatively supercoiled DNA by performing a transient single stranded nick which is then religated at the end of the reaction. It has been shown that the enzyme is located in regions of the genome that are undergoing active RNA synthesis, where it probably reduces superhelical stresses in the DNA, enabling RNA polymerase to function properly. Both DNA topoisomerases I and II have been found to be targets of autoantibodies in the sera of patients with certain autoimmune diseases such as systemic lupus erythematosus and also of some anti tumor drugs and antibiotics. Elevated levels of DNA topoisomerase I, detected by transfer assays, have been demonstrated in colorectal tumors compared with normal colon mucosa as a result of increased transcription or mRNA stability.Synonyms: DNA topoisomerase 2-beta, DNA topoisomerase II beta isozyme
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分子量
- approx. 183 kDa
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基因ID
- 7155
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NCBI登录号
- NP_001059
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UniProt
- Q02880
抗原
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