NCK2 抗体
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Quick Overview for NCK2 抗体 (ABIN271941)
抗原
See all NCK2 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of NCK2 protein. (region surrounding Ile364)
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交叉反应 （详细）
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Species reactivity (expected):Mouse.
Species reactivity (tested):Human. -
纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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纯度
- > 95 % pure by SDS-PAGE
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anti-NCK Adaptor Protein 2 (NCK2) antibody
NCK2 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-NCK Adaptor Protein 2 (NCK2) (AA 1-380) antibodyNCK2 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-NCK Adaptor Protein 2 (NCK2) antibodyNCK2 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB105 unconjugated Recombinant Antibody
anti-NCK Adaptor Protein 2 (NCK2) (AA 1-380) antibodyNCK2 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-NCK Adaptor Protein 2 (NCK2) (AA 63-92), (N-Term) antibodyNCK2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB33920 unconjugated
anti-NCK Adaptor Protein 2 (NCK2) (AA 203-312) antibodyNCK2 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-NCK Adaptor Protein 2 (NCK2) (AA 1-286) antibodyNCK2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1: 1000approx. 1: 5000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1,0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- DO NOT FREEZE!
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted at 2-8 °C.
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- NCK2 (NCK Adaptor Protein 2 (NCK2))
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别名
- NCK2
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背景
- The NCK family of SH2/SH3 adaptor proteins consists of two members, NCK1 (NCKα) and NCK2 (NCKβ), which couple tyrosine kinase signaling, including the EGF and PDGF receptor-pathways, to downstream signaling proteins. Specifically, overexpression of NCK1 in NIH/3T3 cells decreases DNA synthesis stimulated by EGF. Furthermore, the SH2 domain of NCK2 inhibits EGF- and PDGF-induced DNA synthesis. The SH3 domain of NCK binds a proline-rich domain on PAK, a known actin cytoskeleton regulator. The NCK protein thus mediates the interaction between PAK and RAC. The NCK2 protein binds human PDGFR-b (Tyr-1009), overexpression of NCK2 inhibits PDGF-induced membrane ruffling and lamellipod formation. Various growth factor receptors, cell surface antigens and adhesion molecules phosphorylate mammalian NCK1 and NCK2.The human NCK1 and NCK2 genes map to chromosomes 3q21 and 2q12, respectively.Synonyms: NCK adaptor protein 2, SH2/SH3 adaptor protein NCK-beta
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分子量
- approx. 47 kDa
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基因ID
- 8440
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NCBI登录号
- NP_001004720
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UniProt
- O43639
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途径
- EGFR Signaling Pathway, Regulation of Actin Filament Polymerization
抗原
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