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Caldesmon 抗体

The 兔 多克隆 anti-Caldesmon antibody (ABIN271923) specifically detects Caldesmon in WB, IF 和 IHC (p). The antibody is reactive with 人, 大鼠 和 小鼠 samples.
产品编号 ABIN271923
发货至: 中国
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Quick Overview for Caldesmon 抗体 (ABIN271923)

抗原

See all Caldesmon (CALD1) 抗体
Caldesmon (CALD1) (Caldesmon 1 (CALD1))

适用

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人, 大鼠, 小鼠

宿主

  • 132
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克隆类型

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多克隆

标记

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This Caldesmon antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 特异性

    This antibody detects endogenous levels of Caldesmon protein. (region surrounding Ser783)

    交叉反应 (详细)

    Species reactivity (expected):Mouse and Rat.
    Species reactivity (tested):Human.

    纯化方法

    Affinity Chromatography using epitope-specific immunogen.
  • 应用备注

    ELISA: 1/5000approx. 1/10000. Western Blots: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/200. Immunofluorescence: 1/50approx. 1/200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Phosphate buffered saline (PBS), pH ~7.2, 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    Caldesmon (CALD1) (Caldesmon 1 (CALD1))

    别名

    Caldesmon

    背景

    Caldesmon, Filamin 1, Nebulin and Villin are differentially expressed and regulated Actin binding proteins. Both muscular and non-muscular forms of Caldesmon have been identified and each has been shown to bind to Actin as well as to calmodulin and Myosin. Alternative splicing of the gene encoding Caldesmon results in five isoforms. Muscular Caldesmon (isoform 1), also designated high molecular weight Caldesmon or H-Caldesmon (H-CAD), is expressed predominantly on thin filaments in smooth muscle. Non-muscular Caldesmon (isoforms 2-5), also designated low molecular weight Caldesmon or L-Caldesmon (L-CAD), is widely expressed in non-muscle tissues and cells.Synonyms: CAD, CALD1, CDM

    分子量

    70-80 kDa non-muscle 120-150 kDa smooth muscle

    基因ID

    800

    NCBI登录号

    NP_004333

    UniProt

    Q05682

    途径

    Myometrial Relaxation and Contraction
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