SIRPA 抗体
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北京 101111
Quick Overview for SIRPA 抗体 (ABIN271815)
抗原
See all SIRPA 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of Sirp alpha1 protein. (region surrounding Arg479)
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Affinity Chromatography using epitope-specific immunogen.
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anti-Signal-Regulatory Protein alpha (SIRPA) (AA 28-507) antibody
SIRPA 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4C7 unconjugated
anti-Signal-Regulatory Protein alpha (SIRPA) (AA 235-373) antibodySIRPA 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 2H7E2 unconjugated
anti-Signal-Regulatory Protein alpha (SIRPA) (AA 395-504) antibodySIRPA 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Signal-Regulatory Protein alpha (SIRPA) (AA 105-324) antibodySIRPA 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Signal-Regulatory Protein alpha (SIRPA) (AA 56-296) antibodySIRPA 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Signal-Regulatory Protein alpha (SIRPA) (C-Term) antibodySIRPA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Signal-Regulatory Protein alpha (SIRPA) antibodySIRPA 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Signal-Regulatory Protein alpha (SIRPA) (AA 151-250) antibodySIRPA 适用: 人, 小鼠 WB, FACS, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Signal-Regulatory Protein alpha (SIRPA) (AA 1-508) antibodySIRPA 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Signal-Regulatory Protein alpha (SIRPA) (AA 31-373) antibodySIRPA 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid Repeated freeze/thaw cycles.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- SIRPA (Signal-Regulatory Protein alpha (SIRPA))
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别名
- CD172a / SIRPA
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背景
- SIRPs (signal-regulatory proteins) are a family of transmembrane glycoproteins that were identified by their association with the Src homology 2 domaincontaining protein-tyrosine phosphatase SHP-2 in response to insulin. The SIRP family negatively regulates the PI 3-K pathway, which may diminish EGFR-mediated motility and survival phenotypes that contribute to transformation of certain cell types. SIRP-α1 is a transmembrane protein which contains an extracellular portion with three immunoglobulin-like structures and a cytoplasmic region with four potential tyrosine phosphorylation sites. SIRP-α1 is a substrate for activated receptor tyrosine kinases. In its tyrosine phosphorylated form, SIRP-α1 binds to SH-PTP2 through SH2 interactions and acts as an SH-PTP2 substrate. SIRP-α1 has been shown to have negative regulatory effects on cellular responses induced by growth factors, oncogenes and insulin. SIRP-β1 shares extensive sequence homology with SIRP-α1 in its extracellular portion but lacks the cytoplasmic portion. SIRP-γ, originally designated SIRP-β2 (SIRP-B2, CD172g) has unique characteristics from both the α and β versions. SIRP-γ is expressed on the majority of T cells and a proportion of B cells. CD47 associates with SIRP-γ, and this interaction signals unidirectionally only.Synonyms: BIT, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, MFR, MYD1, Macrophage fusion receptor, MyD-1 antigen, PTPNS1, SHP substrate 1, SHPS-1, SHPS1, Signal-regulatory protein alpha-1, Sirp-alpha-2, Sirp-alpha-3, Tyrosine-protein phosphatase non-receptor type substrate 1, p84
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分子量
- approx. 55 kDa
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基因ID
- 140885
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NCBI登录号
- NP_001035111
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UniProt
- P78324
抗原
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