Fc gamma RII (CD32) 抗体
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Quick Overview for Fc gamma RII (CD32) 抗体 (ABIN7304806)
抗原
See all Fc gamma RII (CD32) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse monoclonal antibody to CD32
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特异性
- Recognizes human CD32
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产品特性
- Mouse monoclonal antibody to CD32
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纯化方法
- The antibody was purified by affinity chromatography.
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免疫原
- Native purified human CD32.
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亚型
- IgG2a
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anti-Fc gamma RII (CD32) antibody
CD32 适用: 人 FACS, WB, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal 6D11 unconjugated
anti-Fc gamma RII (CD32) (AA 201-300) antibodyCD32 适用: 人 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Fc gamma RII (CD32) antibody (PE)CD32 适用: 人 FACS 宿主: 小鼠 Monoclonal 3D3 PE
anti-Fc gamma RII (CD32) antibodyCD32 适用: 人 FACS, MS 宿主: 小鼠 Monoclonal 3D3 unconjugated
anti-Fc gamma RII (CD32) (AA 69-309) antibodyCD32 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fc gamma RII (CD32) antibody (PE)GMP Grade CD32 适用: 人 FACS 宿主: 小鼠 Monoclonal IV-3 PE
anti-Fc gamma RII (CD32) antibodyCD32 适用: 人, 犬, 猕猴, Pig FACS, IP, Func, IHC (fro) 宿主: 小鼠 Monoclonal AT10 unconjugated
anti-Fc gamma RII (CD32) antibody (FITC)GMP Grade CD32 适用: 人 FACS 宿主: 小鼠 Monoclonal IV-3 FITC
anti-Fc gamma RII (CD32) antibody (PE)CD32 适用: Cow, 绵羊 FACS 宿主: 小鼠 Monoclonal CCG36 PE
anti-Fc gamma RII (CD32) antibody (FITC)CD32 适用: 人 FACS 宿主: 小鼠 Monoclonal 3D3 FITC
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应用备注
- IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)
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实验流程
- 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Liquid in PBS, pH 7.3, and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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有效期
- 12 months
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- Fc gamma RII (CD32)
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别名
- CD32
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背景
- T3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain, CD antigen CD3d, T3E, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD antigen CD3e, T3G, T-cell surface glycoprotein CD3 gamma chain, T-cell receptor T3 gamma chain, CD antigen CD3g, CD3Z, T3Z, TCRZ, T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain, CD antigen CD247
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基因ID
- 2212
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UniProt
- P12318
抗原
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