WASP 抗体
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Quick Overview for WASP 抗体 (ABIN2689965)
抗原
See all WASP (WAS) 抗体适用
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产品特性
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Wiskott-Aldrich syndrome (WAS) is an X-linked recessive immunodeficiency disease caused by mutations in the gene encoding WAS protein (WASP). The disease is characterized by a spectrum of clinical signs, including thrombocytopenia, eczema, susceptibility to opportunistic and pyogenic infections, and B-cell lymphomas associated with Epstein-Barr virus. Furthermore, patients' blood cells display morphological abnormalities that can be associated with an impaired cytoskeleton. WASP is a member of a family of highly conserved proteins that link signaling pathways to the actin cytoskeleton. These members include WASP, N-WASP (neuronal), and SCAR/WAVE isoforms (Suppressor of cAMP Receptor/WASP family Verprolin-homologous protein) that share two main regions of homology: a proline-rich domain and a carboxyl terminal domain that binds to the Arp2/3 complex. The Arp2/3 complex initiates actin filament assembly in motile cells and formation of the immunological synapse between activated T lymphocytes and antigen-presenting cells. WASP is a central regulator of the actin cytoskeleton in hematopoietic cells that is itself regulated by multiple signaling pathways. The 5A5 antibody recognizes human WASP, it does not cross react with N-WASP. It has been reported to detect WASP in lysates of hematopoietic cells and cell lines, except for neutrophils, from normal donors, but not from a group of patients having mutations of the WAS gene. Western Blot analysis of WASP in human histiocytic lymphoma. Lysate from U937 cells (ATCC CRL-1593.2) was probed with Mouse anti-Human WASP monoclonal antibody at titrations of 0.5 (lane 1), 0.25 (lane 2), and 0.125 μg/mL (lane 3). WASP is identified as a band of 60 kDa.
BD Pharmingen™ Purified Mouse Anti-Human Wiskott-Aldrich Syndrome Protein - Purified - Clone 5A5 - Isotype Mouse IgG2a, κ - Reactivity Hu - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Human WASP Recombinant Protein
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亚型
- IgG2a kappa
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anti-Wiskott-Aldrich Syndrome (Eczema-thrombocytopenia) (WAS) (AA 57-170) antibody
WAS 适用: 人 ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 7B10E4 unconjugated
anti-Wiskott-Aldrich Syndrome (Eczema-thrombocytopenia) (WAS) (AA 39-251) antibodyWAS 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Wiskott-Aldrich Syndrome (Eczema-thrombocytopenia) (WAS) (Internal Region) antibodyVerified WAS 适用: 人 ELISA, IHC, FACS, IF 宿主: 山羊 Polyclonal unconjugated
anti-Wiskott-Aldrich Syndrome (Eczema-thrombocytopenia) (WAS) (AA 145-265) antibodyWAS 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Wiskott-Aldrich Syndrome (Eczema-thrombocytopenia) (WAS) (Internal Region) antibodyWAS 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Wiskott-Aldrich Syndrome (Eczema-thrombocytopenia) (WAS) (AA 129-156), (C-Term) antibodyWAS 适用: 人, 小鼠, 大鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Wiskott-Aldrich Syndrome (Eczema-thrombocytopenia) (WAS) (AA 60-250) antibodyWAS 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Wiskott-Aldrich Syndrome (Eczema-thrombocytopenia) (WAS) (AA 116-144) antibodyWAS 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB23643 unconjugated
anti-Wiskott-Aldrich Syndrome (Eczema-thrombocytopenia) (WAS) (AA 122-152), (Middle Region) antibodyWAS 适用: 人, 小鼠 WB, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Wiskott-Aldrich Syndrome (Eczema-thrombocytopenia) (WAS) (AA 101-200) antibodyWAS 适用: 人, 小鼠 WB, ELISA, ICC, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.25 mg/mL
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缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Mechanisms of WASp-mediated hematologic and immunologic disease." in: Blood, Vol. 104, Issue 12, pp. 3454-62, (2004) (PubMed).
: "Regulation of Wiskott-Aldrich syndrome protein and related molecules." in: Current opinion in cell biology, Vol. 14, Issue 1, pp. 82-7, (2002) (PubMed).
: "Wiskott-Aldrich syndrome protein is required for NK cell cytotoxicity and colocalizes with actin to NK cell-activating immunologic synapses." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 17, pp. 11351-6, (2002) (PubMed).
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: "Mechanisms of WASp-mediated hematologic and immunologic disease." in: Blood, Vol. 104, Issue 12, pp. 3454-62, (2004) (PubMed).
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- WASP (WAS) (Wiskott-Aldrich Syndrome (Eczema-thrombocytopenia) (WAS))
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别名
- Wiskott-Aldrich Syndrome Protein
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背景
- Synonyms: WASP
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分子量
- 60 kDa
抗原
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