T Cell Receptor (TCR) V beta 8 抗体 (Biotin)
Cited in 15+ publications. This anti-T Cell Receptor (TCR) V beta 8 antibody is a 小鼠 单克隆 antibody detecting T Cell Receptor (TCR) V beta 8 in IHC (fro) 和 FACS. Suitable for 小鼠.
(15 references)
产品编号 ABIN2689958
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for T Cell Receptor (TCR) V beta 8 抗体 (Biotin) (ABIN2689958)
抗原
T Cell Receptor (TCR) V beta 8
适用
小鼠
宿主
小鼠
克隆类型
单克隆
标记
Biotin
应用范围
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
克隆位点
F23-1
-
-
产品特性
-
The F23.1 antibody reacts with the Vβ 8.1, Vβ 8.2, and Vβ 8.3 T-cell receptors (TCR) of mice having the b haplotype (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C58, DBA/1, DBA/2) of the Tcrb gene complex. The Tcrb-V8 subfamily gene loci are deleted in mice having the a (e.g., C57BR, C57L, SJL, SWR) or c (e.g., RIII) haplotype. Vβ 8.1 TCR-bearing T lymphocytes are clonally eliminated in mice expressing superantigen coded by Mtv-7 (Mls-1a, Mlsa) provirus (e.g., AKR, CBA/J, C58, DBA/2), and activation or elimination of Vβ 8.1 TCR-expressing T cells by this determinant is partially dependent upon presentation by I-E. Mtv-43 and/or exogenous MMTV-SW superantigens also cause incomplete elimination of Vβ 8.1 TCR-bearing T cells. In addition to expression on conventional T lymphocytes, Vβ 8.2 is the predominant β chain of the TCR on NK-T cells. Staphylococcal enterotoxin B, in association with antigen-presenting cells expressing I-A and/or I-E, stimulates lymphocytes bearing Vβ 8 TCR and selectively eliminates those T cells in vivo. Soluble and plate-bound F23.1 antibody activates Vβ 8 TCR-bearing T cells, soluble antibody blocks cytolysis mediated by Vβ 8 TCR-bearing cytotoxic T lymphocytes, and in vivo treatment of neonatal mice can arrest intrathymic maturation of Vβ 8 TCR-bearing T cells. Two-color analysis of the expression of Vβ 8 TCR on peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with biotin-conjugated F23.1, PE-conjugated RM4-5 (anti-CD4, Cat. No. 553048/553049), and PE-conjugated 53-6.7 (anti-CD8a, Cat. No. 553032/553033) monoclonal antibodies, followed by Avidin-FITC (Cat. No. 554057). Flow cytometry was performed on a FACScan™ 9BDIS, San Jose, CA).
BD Pharmingen™ Biotin Mouse Anti-Mouse Vβ 8 T-Cell Receptor - Biotin - Clone F23.1 - Isotype Mouse IgG2a, κ - Reactivity Ms - 0.25 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
-
免疫原
- BALB.B Mouse Lymph-Node and Spleen T Cells
-
亚型
- IgG2a kappa
-
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
限制
- 仅限研究用
-
-
-
浓度
- 0.5 mg/mL
-
缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
-
储存条件
- 4 °C
-
储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
-
-
-
: "Fas (CD95) participates in peripheral T cell deletion and associated apoptosis in vivo." in: International immunology, Vol. 7, Issue 9, pp. 1451-8, (1996) (PubMed).
: "Peripheral T cells undergoing superantigen-induced apoptosis in vivo express B220 and upregulate Fas and Fas ligand." in: The Journal of experimental medicine, Vol. 183, Issue 2, pp. 431-7, (1996) (PubMed).
: "Mouse NK1+ T cells." in: Current opinion in immunology, Vol. 7, Issue 3, pp. 367-74, (1995) (PubMed).
: "Thymic epithelial cell lines that mediate positive selection can also induce thymocyte clonal deletion." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 3, pp. 1022-31, (1994) (PubMed).
: "Superantigen-like properties of an antibody bispecific for MHC class II molecules and the V beta domain of the T cell antigen receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 8, pp. 3833-41, (1994) (PubMed).
: "TCR-CD4 and TCR-TCR interactions as distinctive mechanisms for the induction of increased intracellular calcium in T-cell signalling." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 3, pp. 1337-45, (1993) (PubMed).
: "Clonal expansion precedes anergy and death of V beta 8+ peripheral T cells responding to staphylococcal enterotoxin B in vivo." in: European journal of immunology, Vol. 21, Issue 8, pp. 1963-6, (1991) (PubMed).
: "RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes." in: The Journal of experimental medicine, Vol. 169, Issue 6, pp. 1903-9, (1989) (PubMed).
: "Antibodies against the T cell receptor/CD3 complex interfere with distinct intra-thymic cell-cell interactions in vivo: correlation with arrest of T cell differentiation." in: European journal of immunology, Vol. 19, Issue 5, pp. 857-63, (1989) (PubMed).
: "The V beta-specific superantigen staphylococcal enterotoxin B: stimulation of mature T cells and clonal deletion in neonatal mice." in: Cell, Vol. 56, Issue 1, pp. 27-35, (1989) (PubMed).
: "In situ localization of T cell receptor beta chain in the murine thymus: changes in the intrathymic distribution of thymocytes expressing beta chain during fetal development." in: Cellular immunology, Vol. 116, Issue 1, pp. 135-48, (1988) (PubMed).
: "Self-tolerance eliminates T cells specific for Mls-modified products of the major histocompatibility complex." in: Nature, Vol. 332, Issue 6159, pp. 35-40, (1988) (PubMed).
: "Expression of a murine polyclonal T cell receptor marker correlates with the use of specific members of the V beta 8 gene segment subfamily." in: The Journal of experimental medicine, Vol. 165, Issue 1, pp. 257-62, (1987) (PubMed).
: "Murine T-cell receptor mutants with deletions of beta-chain variable region genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 3, pp. 767-71, (1986) (PubMed).
: "Characterization of a murine monoclonal antibody specific for an allotypic determinant on T cell antigen receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 134, Issue 6, pp. 3994-4000, (1985) (PubMed).
-
: "Fas (CD95) participates in peripheral T cell deletion and associated apoptosis in vivo." in: International immunology, Vol. 7, Issue 9, pp. 1451-8, (1996) (PubMed).
-
- T Cell Receptor (TCR) V beta 8
-
别名
- Vbeta 8 T-Cell Receptor
抗原
-
You are here:
