T Cell Receptor (TCR) V beta 3 抗体 (Biotin)
This Armenian Hamster 单克隆 antibody specifically detects T Cell Receptor (TCR) V beta 3 in FACS. It exhibits reactivity toward 小鼠. It has been mentioned in 4+ publications
(4 references)
产品编号 ABIN2689955
发货至:
中国
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Quick Overview for T Cell Receptor (TCR) V beta 3 抗体 (Biotin) (ABIN2689955)
抗原
T Cell Receptor (TCR) V beta 3
适用
小鼠
宿主
Armenian Hamster
克隆类型
单克隆
标记
Biotin
应用范围
Flow Cytometry (FACS)
克隆位点
KJ25
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产品特性
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The KJ25 antibody reacts with the Vβ 3 T-cell Receptor (TCR) of strains having the a (e.g., C57BR, SJL), b (e.g., AKR, CBA/Ca, C57BL, DBA/1), and c (e.g., RIII) haplotypes of the Tcrb gene complex. Vβ 3 TCR-bearing T lymphocytes are clonally eliminated either completely or partially in mice expressing superantigens encoded by the Mtv-1 (Mls-4[a], Mls[c]), Mtv-3 (Mls[c]), Mtv-6 (Mls-3[a], Mls[c]), Mtv-13 (Mls-2[a], Mls[c]), Mtv-27, Mtv-44, and/or Mtv-MAI endogenous proviruses (e.g., A, BALB/c, CBA/J, C3H/He, DBA/2, NZB, NZW). Vβ 3 TCR-bearing T cells are activated by the superantigenic Staphylococcal Enterotoxins A and B. Activation or elimination of Vβ 3 TCR-expressing T cells by these determinants is partially dependent upon presentation by I-E. This hamster mAb to a mouse leukocyte antigen does not cross-react with rat leukocytes. Two-color analysis of the expression of VfÀ 3 TCR on peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with biotinylated KJ25(Panel B), PE-conjugated RM4-5 (anti-mouse CD4, Cat. no. 553048/553049, both panels), and PE-conjugated 53-6.7 (anti-mouse CD8a, Cat. no. 553032/553033, both panels) monoclonal antibodies, followed by Avidin-FITC (Cat. no. 554057, both panels). Flow cytometry was performed on a FACScan™ .
BD Pharmingen™ Biotin Hamster Anti-Mouse Vβ 3 T-Cell Receptor - Biotin - Clone KJ25 - Isotype Armenian Hamster IgG2, κ - Reactivity Ms - 0.25 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- alphabeta TCR purified from mouse T-cell hybridoma 2B4.6
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亚型
- IgG2 kappa
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "Profound deletion of mature T cells in vivo by chronic exposure to exogenous superantigen." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 9, pp. 3785-92, (1993) (PubMed).
: "Influence of viral superantigens on V beta- and V alpha-specific positive and negative selection." in: Immunological reviews, Vol. 131, pp. 131-68, (1993) (PubMed).
: "Deletion of self-reactive T cells in nude mice grafted with neonatal allogeneic thymus." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 2, pp. 474-9, (1990) (PubMed).
: "The T-cell repertoire is heavily influenced by tolerance to polymorphic self-antigens." in: Nature, Vol. 335, Issue 6193, pp. 796-801, (1988) (PubMed).
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- T Cell Receptor (TCR) V beta 3
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别名
- Vbeta 3 T-Cell Receptor
抗原
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