T Cell Receptor (TCR) V beta 14 抗体 (Biotin)
There are 6+ publications for this product available. The 大鼠 单克隆 anti-T Cell Receptor (TCR) V beta 14 antibody is suitable to detect T Cell Receptor (TCR) V beta 14 in samples from 小鼠. It has been validated for FACS.
(6 references)
产品编号 ABIN2689953
发货至:
中国
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Quick Overview for T Cell Receptor (TCR) V beta 14 抗体 (Biotin) (ABIN2689953)
抗原
T Cell Receptor (TCR) V beta 14
适用
小鼠
宿主
大鼠
克隆类型
单克隆
标记
Biotin
应用范围
Flow Cytometry (FACS)
克隆位点
14-2
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产品特性
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The 14-2 antibody reacts with the Vβ 14 T-Cell Receptor (TCR) of mice having the a (e.g., C57BR, C57L, SJL, SWR), b (e.g., A, AKR, BALB/c, CBA, C3H/He, C57BL, C58, DBA/1, DBA/2), and c (e.g., RIII) halpotypes of the Tcrb gene complex. Vβ 14 TCR-expressing T lymphocytes are completely eliminated in mice expressing I-E and the superantigens encoded by Mtv-2 endogenous provirus and/or MMTV-C3H, MMTV-GR, or MMTV-D2.GD exogenous virus. Recognition of these determinants by Vβ 14 TCR-expressing T cells is dependent upon presentation by I-E. Plate bound 14-2 antibody activates Vβ 14 TCR-bearing T cells. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two-color analysis of the expression of Vβ 14 TCR on peripheral T lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with biotinylated 14-2 and PE-conjugated 145-2C11 (anti-CD3e, Cat. No. 553063/553064) monoclonal antibodies, followed by Avidin-FITC (Cat. No. 554057). Flow cytometry was performed on a FACScan™ .
BD Pharmingen™ Biotin Rat Anti-Mouse Vβ 14 T-Cell Receptor - Biotin - Clone 14-2 - Isotype Rat IgM, κ - Reactivity Ms - 0.25 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Mouse B10.A Helper T-Cell
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亚型
- IgM kappa
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "Influence of viral superantigens on V beta- and V alpha-specific positive and negative selection." in: Immunological reviews, Vol. 131, pp. 131-68, (1993) (PubMed).
: "Transgenic mouse mammary tumor virus superantigen expression prevents viral infection." in: Cell, Vol. 69, Issue 4, pp. 637-45, (1992) (PubMed).
: "Clonal deletion of V beta 14-bearing T cells in mice transgenic for mammary tumour virus." in: Nature, Vol. 350, Issue 6315, pp. 207-11, (1991) (PubMed).
: "A superantigen encoded in the open reading frame of the 3' long terminal repeat of mouse mammary tumour virus." in: Nature, Vol. 350, Issue 6315, pp. 203-7, (1991) (PubMed).
: "A maternally inherited superantigen encoded by a mammary tumour virus." in: Nature, Vol. 349, Issue 6309, pp. 524-6, (1991) (PubMed).
: "Positive selection determines T cell receptor V beta 14 gene usage by CD8+ T cells." in: The Journal of experimental medicine, Vol. 170, Issue 1, pp. 135-43, (1989) (PubMed).
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- T Cell Receptor (TCR) V beta 14
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别名
- Vbeta 14 T-Cell Receptor
抗原
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