T Cell Receptor (TCR) V beta 12 抗体 (Biotin)
This 小鼠 单克隆 antibody specifically detects T Cell Receptor (TCR) V beta 12 in FACS. It exhibits reactivity toward 小鼠. It has been mentioned in 3+ publications
(3 references)
产品编号 ABIN2689952
发货至:
中国
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Quick Overview for T Cell Receptor (TCR) V beta 12 抗体 (Biotin) (ABIN2689952)
抗原
T Cell Receptor (TCR) V beta 12
适用
小鼠
宿主
小鼠
克隆类型
单克隆
标记
Biotin
应用范围
Flow Cytometry (FACS)
克隆位点
MR11
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产品特性
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The MR11-1 antibody reacts with the Vβ 12 T-cell Receptor (TCR) of mice having the b haplotype (e.g., C57BL, C58, DBA/1) of the Tcrb gene complex1 The Tcrb-V12 gene locus is deleted in mice having the a (e.g., C57BR, C57L, SJL, SWR) or c (e.g., RIII) haplotype. Vβ 12 TCR-bearing T lymphocytes are clonally eliminated in mice expressing I-E and superantigens encoded by Mtv-8 (Mlsf, Dvb11.1), Mtv-9 (Etc-1, Mlsf, Dvb11.2) and/or Mtv-11 (Mlsf, Dvb11.3) proviruses (eg, A, AKR, BALB/c, CBA/J, C3H, DBA/2). Activation of Vβ 12 TCR-expressing T cells by these determinants is dependent upon presentation by I-E. C57BL/6 spleen T cells expressing Vβ 12 TCR are among the predominant responders to MAIDS virus superantigen. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two-color analysis of the expression of Vß12 TCR on peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with biotinylated MR11-1, PE-conjugated anti-mouse CD4 RM4-5 (Cat. No. 553048/553049), and PE-conjugated anti-mouse CD8a 53-6.7 (Cat. No. 553032/553033) monoclonal antibodies, followed by Avidin-FITC, Cat. No. 554057). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
BD Pharmingen™ Biotin Mouse Anti-Mouse Vβ 12 T-Cell Receptor - Biotin - Clone MR11-1 - Isotype Mouse IgG1, κ - Reactivity Ms - 0.25 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Not Reported
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亚型
- IgG1 kappa
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "Selective decreases in T cell receptor V beta expression. Decreased expression of specific V beta families is associated with expression of multiple MHC and non-MHC gene products." in: The Journal of experimental medicine, Vol. 170, Issue 4, pp. 1335-46, (1989) (PubMed).
: "RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes." in: The Journal of experimental medicine, Vol. 169, Issue 6, pp. 1903-9, (1989) (PubMed).
: "Murine T-cell receptor mutants with deletions of beta-chain variable region genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 3, pp. 767-71, (1986) (PubMed).
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- T Cell Receptor (TCR) V beta 12
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别名
- Vbeta 12 T-Cell Receptor
抗原
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