T Cell Receptor (TCR) V beta 11 抗体 (Biotin)
Cited in 5+ publications.
The Biotin-conjugated 大鼠 单克隆 anti-T Cell Receptor (TCR) V beta 11 antibody (Clone RR3) (ABIN2689951) specifically detects T Cell Receptor (TCR) V beta 11 in FACS.
The antibody is reactive with 小鼠 samples.
(5 references)
产品编号 ABIN2689951
发货至:
中国
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Quick Overview for T Cell Receptor (TCR) V beta 11 抗体 (Biotin) (ABIN2689951)
抗原
T Cell Receptor (TCR) V beta 11
适用
小鼠
宿主
大鼠
克隆类型
单克隆
标记
Biotin
应用范围
Flow Cytometry (FACS)
克隆位点
RR3
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产品特性
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The RR3-15 antibody reacts with the Vβ 11 T-Cell Receptor (TCR) of mice having the b haplotype (e.g., A, C57BL, C58, DBA/1) of the Tcrb gene complex. The Tcrb-V11 gene locus is deleted in mice having the a (e.g., C57BR, C57L, SJL, SWR) and c (e.g., RIII) haplotypes. Vβ TCR-bearing T lymphocytes are clonally eliminated in mice expressing I-E and superantigens encoded by Mtv-9 (Etc-1, Mls[f], Dvb11.2) and/or Mtv-11 (Mls[f], Dvb 11.2) proviruses (e.g., AKR, BALB/c, CBA/J, C3H, DBA/2), and they are incompletely eliminated in mice expressing I-E and Mtv-8 (Mls[f], Dvb 11.1) superantigen (e.g., A). Activation of Vβ 11 TCR-expressing T cells by these determinants is dependent upon presentation by I-E. The bacterial superantigen Staphylococcal enterotoxin A (SEA) also interacts with Vβ 11 TCR, and in vivo exposure to SEA causes activation and subsequent deletion of Vβ TCR-expressing lymphocytes. Plate-bound RR3-15 antibody activates Vβ 11 TCR-bearing T cells. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two-color analysis of the expression of Vβ 11 TCR on peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with biotin-conjugated RR3-15, PE-conjugated RM4-5 (anti-CD4, Cat. No. 553048/553049), and PE-conjugated 536.7 (anti-CD8a, Cat. No. 553032/553033) monoclonal antibodies, followed by Avidin-FITC (Cat. No. 554057). Flow cytometry was performed on a FACScan™ .
BD Pharmingen™ Biotin Rat Anti-Mouse Vβ 11 T-Cell Receptor - Biotin - Clone RR3-15 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.25 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Mouse Cytolytic T-Cell
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亚型
- IgG2b kappa
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "Profound deletion of mature T cells in vivo by chronic exposure to exogenous superantigen." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 9, pp. 3785-92, (1993) (PubMed).
: "Capacity of unprimed CD4+ and CD8+ T cells expressing V beta 11 receptors to respond to I-E alloantigens in vivo." in: The Journal of experimental medicine, Vol. 170, Issue 6, pp. 1947-57, (1990) (PubMed).
: "The MHC molecule I-E is necessary but not sufficient for the clonal deletion of V beta 11-bearing T cells." in: The Journal of experimental medicine, Vol. 169, Issue 4, pp. 1405-19, (1989) (PubMed).
: "RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes." in: The Journal of experimental medicine, Vol. 169, Issue 6, pp. 1903-9, (1989) (PubMed).
: "Murine T-cell receptor mutants with deletions of beta-chain variable region genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 3, pp. 767-71, (1986) (PubMed).
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- T Cell Receptor (TCR) V beta 11
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别名
- Vbeta 11 T-Cell Receptor
抗原
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