SOX1 抗体
(4 references)Quick Overview for SOX1 抗体 (ABIN2689905)
抗原
See all SOX1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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品牌
- BD Pharmingen™
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产品特性
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The N23-844 monoclonal antibody reacts with human Sox1, a member of the SOX [SRY (sex determining region Y)-HMG-box] family of transcription factors. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. It is one of the earliest transcription factors to be expressed in ectodermal cells committed to the neural fate. Sox1 is expressed in both embryonic and somatic neural stem and progenitor cells, and it is down regulated during neuronal differentiation in many neuronal subtypes. Western Blot analysis of SOX1. Lysate from neural stem cells (NSCs) derived from H9 human ES cells (WiCell, Madison, WI) was probed with Purified Mouse anti-Human SOX1 monoclonal antibody at titrations of 0.5 (lane 1), 0.25 (lane 2), and 0.125 μg/mL (lane 3). SOX1 is identified as a tight doublet of almost 39 kDa. Immunofluorescent staining of Human SOX1. NSCs derived from H9 human ES cells (WiCell Madison, WI) were fixed, permeabilized with BD Perm/Wash™ Buffer (Cat No. 554723), and stained with purified anti-human SOX1 (0.5 μg/test). The second step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies) and counter-staining was with Hoechst 33342 (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ Software. Triton™ X-100 is also suitable for permeabilization.
BD Pharmingen™ Purified Mouse anti-Human SOX1 - Purified - Clone N23-844 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Human Sox1 Peptide
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亚型
- IgG1 kappa
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anti-SRY (Sex Determining Region Y)-Box 1 (SOX1) antibody
SOX1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Monoclonal 8E1 unconjugated Recombinant Antibody
anti-SRY (Sex Determining Region Y)-Box 1 (SOX1) antibodySOX1 适用: 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 1 (SOX1) (AA 100-150) antibodySOX1 适用: 人, 小鼠, 猴, 马, 非洲爪蟾, Opossum WB 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 1 (SOX1) (Middle Region) antibodySOX1 适用: 人, 小鼠, 兔, 斑马鱼, Cow, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 1 (SOX1) (N-Term) antibodySOX1 适用: 人, 小鼠, Cow, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 1 (SOX1) (AA 313-362) antibodySOX1 适用: 人, 小鼠, 兔, 犬, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 1 (SOX1) (AA 47-76) antibodySOX1 适用: 人 WB, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 1 (SOX1) (AA 1-485) antibodySOX1 适用: 斑马鱼 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 1 (SOX1) antibodySOX1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 1 (SOX1) (AA 49-68) antibodySOX1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Sox1 acts through multiple independent pathways to promote neurogenesis." in: Developmental biology, Vol. 269, Issue 2, pp. 580-94, (2004) (PubMed).
: "Matching SOX: partner proteins and co-factors of the SOX family of transcriptional regulators." in: Current opinion in genetics & development, Vol. 12, Issue 4, pp. 441-6, (2002) (PubMed).
: "A role for SOX1 in neural determination." in: Development (Cambridge, England), Vol. 125, Issue 10, pp. 1967-78, (1998) (PubMed).
: "Cloning and mapping of the human SOX1: a highly conserved gene expressed in the developing brain." in: Mammalian genome : official journal of the International Mammalian Genome Society, Vol. 8, Issue 11, pp. 866-8, (1998) (PubMed).
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: "Sox1 acts through multiple independent pathways to promote neurogenesis." in: Developmental biology, Vol. 269, Issue 2, pp. 580-94, (2004) (PubMed).
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- SOX1 (SRY (Sex Determining Region Y)-Box 1 (SOX1))
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别名
- SOX1
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分子量
- 39 kDa
抗原
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