SIGLEC5 抗体
(2 references)-
- 抗原 See all SIGLEC5 抗体
- SIGLEC5 (Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This SIGLEC5 antibody is un-conjugated
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应用范围
- ELISA, Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
- 品牌
- BD Pharmingen™
- 产品特性
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The E50-2440 antibody reacts with Siglec-F, the sixth siglec protein to be reported in the mouse. Siglecs are the sialic acid-binding immunoglobulin superfamily lectins defined in the human, each of which has a distinctive expression pattern in the hematopoietic system and at least some of which are known to mediate cell-cell interactions. Orthologous proteins of human Siglec-1 (Sialoadhesin or CD169), Siglec-2 (CD22), and Siglec-4 (myelin-associated glycoprotein) have been characterized in the mouse. Human Siglec-3 (CD33) and Siglecs-5 through -10 are encoded by a cluster of closely related genes, and each has two cytoplasmic ITIM (Immunoreceptor Tyrosine-based Inhibitory Motifs). Similarly, mouse Siglec-F is encoded by a gene in a syntenic cluster in the mouse, and the protein has sialic acid-binding activity and an intracytoplasmic ITIM. Its expression pattern differs from those of the human Siglec-3-related proteins in that it is found on immature cells of the myelomonocytic lineage, with reduced expression on mature neutrophils and monocytes, and not on lymphoid cells. It has been proposed that mAb E50-2440 may be used for identification of immature myelomonocytic cells in the mouse. Two-color analysis of Siglec-F expression on bone-marrow myeloid cells. BALB/c bone-marrow leukocytes were stained with either purified rat IgG2a, κ isotype control mAb R35-95 (Cat. No. 553927, left panel) or purified mAb E50-2440 (right panel) in the presence of Mouse Fc Block™ (purified anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142), followed by biotinylated anti-rat IgG2a mAb RG7/1.30 (Cat. No. 553894), then Streptavidin-PE (Cat. No. 554061) and FITC-conjugated anti-mouse CD11b (Integrin αM chain) mAb M1/70 (Cat. No. 557396/553310). Flow cytometry was performed on a FACSCalibur™ .
BD Pharmingen™ Purified Rat Anti-Mouse Siglec-F - Purified - Clone E50-2440 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Mouse Siglec-F and human IgG Fc recombinant fusion protein
- 克隆位点
- E50
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product SIGLEC5 Primary Antibody
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anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (AA 17-230) antibody
SIGLEC5 适用: 人 WB, ELISA, IHC, FACS, ICC, Neut 宿主: 小鼠 Monoclonal 3F5A3 unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) antibodySIGLEC5 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (N-Term) antibodySIGLEC5 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (C-Term) antibodySIGLEC5 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (Extracellular Domain) antibody (APC)SIGLEC5 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal 1A5 APC
anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (AA 147-335) antibodySIGLEC5 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (N-Term) antibodySIGLEC5 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (Extracellular Domain) antibodySIGLEC5 适用: 人, Non-Human Primate ELISA, IP, FACS, ICC, IHC (fro) 宿主: 小鼠 Monoclonal 1A5 unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (AA 458-551) antibody (Biotin)SIGLEC5 适用: 人 WB 宿主: 兔 Polyclonal Biotin
anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (Extracellular Domain) antibody (PE)SIGLEC5 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal 1A5 PE
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Cloning and characterization of a novel mouse Siglec, mSiglec-F: differential evolution of the mouse and human (CD33) Siglec-3-related gene clusters." in: The Journal of biological chemistry, Vol. 276, Issue 48, pp. 45128-36, (2001) (PubMed).
: "Siglecs, sialic acids and innate immunity." in: Trends in immunology, Vol. 22, Issue 6, pp. 337-42, (2001) (PubMed).
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: "Cloning and characterization of a novel mouse Siglec, mSiglec-F: differential evolution of the mouse and human (CD33) Siglec-3-related gene clusters." in: The Journal of biological chemistry, Vol. 276, Issue 48, pp. 45128-36, (2001) (PubMed).
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- 抗原
- SIGLEC5 (Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5))
- 别名
- Siglec-F (SIGLEC5 产品)
- 别名
- CD170 antibody, CD33L2 antibody, OB-BP2 antibody, OBBP2 antibody, SIGLEC-5 antibody, sialic acid binding Ig like lectin 5 antibody, sialic acid-binding Ig-like lectin 5 antibody, sialic acid binding Ig-like lectin 5 antibody, SIGLEC5 antibody, LOC100597560 antibody, Siglec5 antibody
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