ROR gamma T/RORC2/NR1F3 抗体
Cited in 9+ publications. This anti-ROR gamma T/RORC2/NR1F3 antibody is a 小鼠 单克隆 antibody detecting ROR gamma T/RORC2/NR1F3 in WB, IHC (f), IHC (fro), ICS 和 IHC (p). Suitable for 人 和 小鼠.
(9 references)
产品编号 ABIN2689894
发货至:
中国
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Quick Overview for ROR gamma T/RORC2/NR1F3 抗体 (ABIN2689894)
抗原
ROR gamma T/RORC2/NR1F3
适用
人, 小鼠
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Staining (ICS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
克隆位点
Q31
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产品特性
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The Q31-378 monoclonal antibody recognizes RORgamma t (RORγt), an isoform of RORgamma (RORγ). RORγt is a DNA-binding transcription factor that belongs to the ROR/RZR orphan nuclear receptor family. RORγt is expressed exclusively by lymphoid cells including CD4+CD8+ thymocytes, peripheral CD4+ Th17 and CD8+ Tc17 cells, NKT cells and innate lymphoid cells such as lymphoid tissue inducer (LTi) cells. RORγt plays essential roles in thymopoiesis, T cell homeostasis, differentiation of effector T lymphocytes and the development of secondary lymphoid tissues including lymph nodes and Peyer's patches. Analyses of RORγt Expression by Western bloting and Immunohistochemistry. Left Panel: Western blot analyses of RORγt expression by mouse splenocytes and thymocytes. Cell lysates from untreated mouse splenocytes (Spl, Lanes 1,2) and thymocytes (Thy, Lanes 3,4) (20 μg total cellular protein/lane) were electrophoresed (SDS-PAGE) and transferred to membranes. They were then probed with Purified Anti-Actin antibody (Cat. No. 612656/612657, Lanes 1,3 at 1 μg of antibody/mL) or Purified Mouse Anti-Mouse RORγt antibody (
BD Pharmingen™ Purified Mouse anti-Mouse RORγt - Purified - Clone Q31-378 - Isotype Mouse IgG2a, κ - Reactivity Ms, Hu - 50 μg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Mouse RORgammat Recombinant Protein
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亚型
- IgG2a kappa
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "RORC2: the master of human Th17 cell programming." in: European journal of immunology, Vol. 39, Issue 6, pp. 1452-5, (2009) (PubMed).
: "Tc17, a unique subset of CD8 T cells that can protect against lethal influenza challenge." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 6, pp. 3469-81, (2009) (PubMed).
: "The full-ORF clone resource of the German cDNA Consortium." in: BMC genomics, Vol. 8, pp. 399, (2008) (PubMed).
: "The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+ T helper cells." in: Cell, Vol. 126, Issue 6, pp. 1121-33, (2006) (PubMed).
: "The role of the nuclear hormone receptor RORgammat in the development of lymph nodes and Peyer's patches." in: Immunological reviews, Vol. 195, Issue 9, pp. 81-90, (2003) (PubMed).
: "An essential function for the nuclear receptor RORgamma(t) in the generation of fetal lymphoid tissue inducer cells." in: Nature immunology, Vol. 5, Issue 1, pp. 64-73, (2003) (PubMed).
: "RORgamma t, a novel isoform of an orphan receptor, negatively regulates Fas ligand expression and IL-2 production in T cells." in: Immunity, Vol. 9, Issue 6, pp. 797-806, (1999) (PubMed).
: "Cloning of a cDNA encoding the murine orphan receptor RZR/ROR gamma and characterization of its response element." in: Gene, Vol. 181, Issue 1-2, pp. 199-206, (1997) (PubMed).
: "ROR gamma: the third member of ROR/RZR orphan receptor subfamily that is highly expressed in skeletal muscle." in: Biochemical and biophysical research communications, Vol. 205, Issue 3, pp. 1976-83, (1995) (PubMed).
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: "RORC2: the master of human Th17 cell programming." in: European journal of immunology, Vol. 39, Issue 6, pp. 1452-5, (2009) (PubMed).
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- ROR gamma T/RORC2/NR1F3
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别名
- RORgammat
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背景
- Synonyms: RORγT, RORgt, RORgamma t, RORgammaT, Rorc2, Rorg, TOR, Thor, Nr1f3
抗原
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