CCL5 抗体
(2 references)Quick Overview for CCL5 抗体 (ABIN2689891)
抗原
See all CCL5 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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品牌
- BD Pharmingen™
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产品特性
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The 2D5 antibody reacts with human Regulated upon Activation, Normal T cells Expressed and Secreted (RANTES). The immunogen used to generated this hybridoma was recombinant human RANTES protein. Immunocytochemical staining for RANTES. PBMC were isolated from human peripheral blood by density gradient centrifugation and were cultured overnight at 37 °C with LPS (Sigma Cat. No. L-8274, 1 μg / ml) in the presence of BD GolgiStop™ (Cat. No. 554724). The activated cells were harvested and stained by immunocytochemistry using a three-step staining procedure employing a Biotin Goat Anti-Mouse Ig secondary antibody and a Horseradish Peroxidase (HRP) based detection system (Nomarski optics, original magnification 400X).
BD Pharmingen™ Purified Mouse Anti-Human RANTES - Purified - Clone 2D5 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.25 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Recombinant human RANTES protein
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亚型
- IgG1 kappa
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anti-Chemokine (C-C Motif) Ligand 5 (CCL5) (AA 24-91) antibody
CCL5 适用: 犬 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 5 (CCL5) (AA 24-91) antibodyCCL5 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 5 (CCL5) (AA 62-91) antibodyCCL5 适用: 人, 小鼠, 豚鼠, Pig ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 5 (CCL5) (AA 24-91) antibodyCCL5 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 5 (CCL5) antibodyCCL5 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 5 (CCL5) antibodyCCL5 适用: 人 WB, ELISA, Func, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 5 (CCL5) antibody (Biotin)CCL5 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal Biotin
anti-Chemokine (C-C Motif) Ligand 5 (CCL5) (AA 24-91) antibodyCCL5 适用: 猕猴 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 5 (CCL5) (AA 24-91) antibodyCCL5 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Detection of the chemokine RANTES in cytokine-stimulated human dermal fibroblasts." in: The Journal of investigative dermatology, Vol. 105, Issue 4, pp. 585-91, (1995) (PubMed).
: "Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 29, Issue 4, pp. 577-80, (1981) (PubMed).
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: "Detection of the chemokine RANTES in cytokine-stimulated human dermal fibroblasts." in: The Journal of investigative dermatology, Vol. 105, Issue 4, pp. 585-91, (1995) (PubMed).
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- CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
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别名
- RANTES
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途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration
抗原
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