Pan Carcinoma 抗体
Cited in 4+ publications.
The 小鼠 单克隆 anti-Pan Carcinoma antibody (Clone KS1-4) (ABIN2689873) specifically detects Pan Carcinoma in IHC (fro), FACS, IF 和 IP.
The antibody is reactive with 人 samples.
(4 references)
产品编号 ABIN2689873
发货至:
中国
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Quick Overview for Pan Carcinoma 抗体 (ABIN2689873)
抗原
Pan Carcinoma
适用
人
宿主
小鼠
克隆类型
单克隆
应用范围
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)
克隆位点
KS1-4
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产品特性
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KS1/4 antigen (Ag), defined by the KS1/4 antibody (KS1/4), is expressed in many epithelial-derived carcinomas and normal epithelial cell surfaces. The cDNA encoding KS1/4 has been isolated and contains an open reading frame of 314 amino acids including a putative signal sequence. KS1/4 Ag migrates as three glycosylated polypeptides with molecular masses of 35, 40 and 42 kDa. The 40 and 42 kDa species are similar proteins and appear to differ only by their degree of N-linked glycosylation. The 35 kDa species results from proteolytic cleavages of the larger molecular weight proteins. KS1/4 was first described as a monoclonal antibody which recognized a lung adenocarcinoma associated antigen. In oncolytic drug targeting studies, KS1/4 suppressed the growth of human lung tumor xenografts in athymic nude mice. Subsequent studies showed that KS1/4 reacts with a variety of tumor tissues including colon, breast, ovarian, and pancreas. It also recognizes normal epithelium including colon, stomach, small intestine, liver, kidney, lung, pancreas, skin and ovary. These results suggest that KS1/4 Ag represents an epithelial cell/epithelial-derived carcinoma marker. KS1/4 recognizes a 40 - 42 kDa antigen expressed on the cell surfaces of a variety of epithelial tumors and normal epithelial cell types. KS1/4 also recognizes a 35 kDa proteolytic fragment. UCLA-P3 cells derived from a human adenocarcinoma of the lung were used as immunogen. Profile of UCLA-P3 lung adenocarcinoma cells analyzed by flow cytometry. Cells were incubated with either KS1/4 (Cat. No. 554276) or an isotype control, followed by a FITC-conjugated second step antibody.
BD Pharmingen™ Purified Mouse Anti-40 kDa Pan Carcinoma - Purified - Clone KS1/4 - Isotype Mouse IgG2a - Reactivity Hu - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- UCLA-P3 cells
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亚型
- IgG2a
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Isolation and characterization of a cDNA encoding the KS1/4 epithelial carcinoma marker." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 10, pp. 3662-7, (1989) (PubMed).
: "Characterization of the human tumor and normal tissue reactivity of the KS1/4 monoclonal antibody." in: Hybridoma, Vol. 7, Issue 4, pp. 407-15, (1988) (PubMed).
: "Disposition of the monoclonal antibody-vinca alkaloid conjugate KS1/4-DAVLB (LY256787) and free 4-desacetylvinblastine in tumor-bearing nude mice." in: The Journal of pharmacology and experimental therapeutics, Vol. 241, Issue 2, pp. 695-703, (1987) (PubMed).
: "Antigens associated with a human lung adenocarcinoma defined by monoclonal antibodies." in: Cancer research, Vol. 44, Issue 2, pp. 681-7, (1984) (PubMed).
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- Pan Carcinoma
抗原
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