Mast Cells 抗体
This 小鼠 单克隆 antibody specifically detects Mast Cells in FACS, IP, Sep 和 IHC. It exhibits reactivity toward 大鼠. It has been mentioned in 3+ publications
(3 references)
产品编号 ABIN2689832
发货至:
中国
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Quick Overview for Mast Cells 抗体 (ABIN2689832)
抗原
Mast Cells
适用
大鼠
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Flow Cytometry (FACS), Immunoprecipitation (IP), Separation (Sep), Immunohistochemistry (IHC)
克隆位点
AR32AA4
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产品特性
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The AR32AA4 (AA4) antibody reacts with two distinct α-galactosyl derivatives of the ganglioside GD1b (disialogangliosides). The mAb AA4 reacts with four indistinct bands ranging in size from 56 - 110 kDa. Binding of the AA4 antibody to RBL-2H3 cells has been shown to inhibit IgE-mediated histamine release by inhibiting FcεR1 signal transduction events. The mAb AA4 has been shown to be useful for immunomagnetic mast cell separation from rat bone marrow and peritoneal lavage cell populations. This corresponds with reports that the AA4-binding epitope is expressed at higher levels than the FcεR1 on RBL-2H3 cells. Staining of mAb AR32AA4 on RBL (ATCC CRL-2256) cells. RBL cells were incubated with either Purified Mouse IgG1, κ isotype control (Cat. no. 557273, open dash line overlay) or purified AR32AA4 mAb (shaded histogram), followed by biotinylated F(ab')2 rat antimouse IgG and finally Streptavidin-FITC (Cat. no. 554060). The cells in both the shaded histogram and the overlay were gated on 7-AAD negative cells. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
BD Pharmingen™ Purified Mouse Anti-Rat Mast Cells - Purified - Clone AR32AA4 (also known as AA4) - Isotype Mouse IgG1, κ - Reactivity Rat - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Rat basophilic leukemia cell line
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亚型
- IgG1 kappa
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Immunomagnetic isolation of rat bone marrow-derived and peritoneal mast cells." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 45, Issue 12, pp. 1715-22, (1998) (PubMed).
: "Anti-ganglioside monoclonal antibody AA4 selectively inhibits IgE-induced signal transduction pathways in rat basophilic leukemia cells." in: Molecular immunology, Vol. 34, Issue 3, pp. 227-35, (1997) (PubMed).
: "Monoclonal antibodies that inhibit IgE binding." in: The Journal of biological chemistry, Vol. 261, Issue 25, pp. 11823-31, (1986) (PubMed).
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- Mast Cells
抗原
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