LY6C + LY6G 抗体 (Biotin)
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北京 101111
Quick Overview for LY6C + LY6G 抗体 (Biotin) (ABIN2689825)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
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The RB6-8C5 antibody reacts with a common epitope on Ly-6G and Ly-6C, previously known as the myeloid differentiation antigen Gr-1. In the bone marrow, the level of antigen expression is directly correlated with granulocyte differentiation and maturation. The antigen is also expressed on the monocyte lineage in the bone marrow, but not on erythroid cells. In the periphery, RB6-8C5 antibody recognizes granulocytes (neutrophils and eosinophils) and monocytes. The RB6-8C5 mAb is a component of the "lineage cocktail" used in studies of hematopoietic lineages. The mAb 1A8 (Cat. No. 551461) specifically recognizes Ly-6G, but not Ly-6C. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.
BD Pharmingen™ Biotin Rat Anti-Mouse Ly-6G and Ly-6C - Biotin - Clone RB6-8C5 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Not Reported
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亚型
- IgG2b kappa
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anti-LY6C + LY6G antibody (PE)
适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 PE
anti-LY6C + LY6G antibody适用: 小鼠 FACS, IHC, WB, Dep 宿主: 兔 Chimeric RB6-8C5 unconjugated Recombinant Antibody
anti-LY6C + LY6G antibody (FITC)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 FITC
anti-LY6C + LY6G antibody (Cy5)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 Cy5
anti-LY6C + LY6G antibody (PE-Cy7)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 PE-Cy7
anti-LY6C + LY6G antibody (Biotin)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 Biotin
anti-LY6C + LY6G antibody (APC-Cy7)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 APC-Cy7
anti-LY6C + LY6G antibody (PE-Cy5.5)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 PE-Cy5.5
anti-LY6C + LY6G antibody (SPRD)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 SPRD
anti-LY6C + LY6G antibody (APC)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 APC
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "Flow cytometric identification of murine neutrophils and monocytes." in: Journal of immunological methods, Vol. 197, Issue 1-2, pp. 139-50, (1996) (PubMed).
: "Neutrophils are essential for early anti-Listeria defense in the liver, but not in the spleen or peritoneal cavity, as revealed by a granulocyte-depleting monoclonal antibody." in: The Journal of experimental medicine, Vol. 179, Issue 1, pp. 259-68, (1994) (PubMed).
: "Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 5, pp. 2399-408, (1993) (PubMed).
: "An eosinophil-dependent mechanism for the antitumor effect of interleukin-4." in: Science (New York, N.Y.), Vol. 257, Issue 5069, pp. 548-51, (1992) (PubMed).
: "Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 1, pp. 22-8, (1991) (PubMed).
: "Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon-gamma." in: European journal of immunology, Vol. 18, Issue 11, pp. 1819-26, (1989) (PubMed).
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- LY6C + LY6G
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别名
- Ly-6G and Ly-6C
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背景
- Synonyms: Gr-1
抗原
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