(11 references)-
- 抗原 See all Sca-1/Ly-6A/E (Ly6a) 抗体
- Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This Sca-1/Ly-6A/E antibody is conjugated to Biotin
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应用范围
- Flow Cytometry (FACS)
- 品牌
- BD Pharmingen™
- 产品特性
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The D7 antibody reacts with Ly-6A.2 and Ly-6E.1, which are allelic members of the Ly-6 multigene family. Sca-1 (Ly6A/E), a phosphatidylinositol-anchored protein of about 18 kDa, is expressed on the multipotent hematopoietic stem cells (HSC) in the bone marrow of mice with both Ly-6 haplotypes. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, C57L, C58, DBA/2, PL, SJL, SWR, 129), Ly-6A/E is also expressed on distinct subpopulations of bone marrow and peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains with the Ly-6.1 haplotype (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes, whereas activation of lymphocytes from mice of both Ly-6 haplotypes leads to strong expression of the Sca-1 antigen. Studies with the D7 antibody have demonstrated that Ly-6A/E may be involved in the regulation of B and T lymphocyte responses, and it appears to be required for T-cell receptor-mediated T-cell activation. Purified E13-161.7 mAb (anti-Ly-6A/E, Cat. No. 553333) can block binding of FITC-conjugated D7 antibody (Cat. No. 557405) to mouse splenocytes, but purified mAb D7 is unable to block binding of FITC-conjugated E13-161.7 antibody (Cat. No. 553335). Anti-Ly-6A/E (Sca-1) mAb may be used in combination with the Mouse Lineage Panel (Cat. No. 559971) to identify HSC. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.
BD Pharmingen™ Biotin Rat Anti-Mouse Ly-6A/E - Biotin - Clone D7 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- IL-2-dependent mouse T-cell line CTL-L
- 克隆位点
- D7
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product Ly6a Primary Antibody
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anti-Lymphocyte Antigen 6 Complex, Locus A (Ly6a) antibody (APC)
Ly6a 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal D7 APC
anti-Lymphocyte Antigen 6 Complex, Locus A (Ly6a) antibodyLy6a 适用: 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Antigen 6 Complex, Locus A (Ly6a) antibodyLy6a 适用: 小鼠, 大鼠, 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Antigen 6 Complex, Locus A (Ly6a) antibody (PE)Ly6a 适用: 小鼠 FACS, WB, IP 宿主: 大鼠 Monoclonal D7 PE
anti-Lymphocyte Antigen 6 Complex, Locus A (Ly6a) antibody (Biotin)Ly6a 适用: 小鼠 FACS, WB, IP 宿主: 大鼠 Monoclonal D7 Biotin
anti-Lymphocyte Antigen 6 Complex, Locus A (Ly6a) antibody (APC)Ly6a 适用: 小鼠 FACS, WB, IP 宿主: 大鼠 Monoclonal D7 APC
anti-Lymphocyte Antigen 6 Complex, Locus A (Ly6a) (AA 91-108) antibodyLy6a 适用: 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Antigen 6 Complex, Locus A (Ly6a) antibodyLy6a 适用: 小鼠 FACS, WB, IP, IHC (fro) 宿主: 大鼠 Monoclonal D7 unconjugated
anti-Lymphocyte Antigen 6 Complex, Locus A (Ly6a) antibody (SPRD)Ly6a 适用: 小鼠 FACS, WB, IP 宿主: 大鼠 Monoclonal D7 SPRD
anti-Lymphocyte Antigen 6 Complex, Locus A (Ly6a) antibody (FITC)Ly6a 适用: 小鼠 FACS, WB, IP 宿主: 大鼠 Monoclonal D7 FITC
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "In vitro expansion of hematopoietic progenitors and maintenance of stem cells: comparison between FLT3/FLK-2 ligand and KIT ligand." in: Blood, Vol. 89, Issue 6, pp. 1915-21, (1997) (PubMed).
: "In vitro differentiation of murine Sca-1+Lin- cells into myeloid, B cell and T cell lineages." in: Stem cells (Dayton, Ohio), Vol. 14, Issue 4, pp. 412-8, (1996) (PubMed).
: "Transplantable NK cell progenitors in murine bone marrow." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 4, pp. 1653-63, (1995) (PubMed).
: "Regulation of B lymphocyte responses to IL-4 and IFN-gamma by activation through Ly-6A/E molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 6, pp. 2197-204, (1990) (PubMed).
: "Down-regulation of IL-2 production by activation of T cells through Ly-6A/E." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 5, pp. 1407-14, (1990) (PubMed).
: "Inhibition of Ly-6A antigen expression prevents T cell activation." in: The Journal of experimental medicine, Vol. 172, Issue 1, pp. 115-20, (1990) (PubMed).
: "Expression of Ly-6A/E alloantigens in thymocyte and T-lymphocyte subsets: variability related to the Ly-6a and Ly-6b haplotypes." in: Immunogenetics, Vol. 29, Issue 2, pp. 98-107, (1989) (PubMed).
: "Tumor necrosis factor synergistically acts with IFN-gamma to regulate Ly-6A/E expression in T lymphocytes, thymocytes and bone marrow cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 6, pp. 1929-36, (1989) (PubMed).
: "Role of Ly-6 in lymphocyte activation. II. Induction of T cell activation by monoclonal anti-Ly-6 antibodies." in: The Journal of experimental medicine, Vol. 164, Issue 3, pp. 709-22, (1986) (PubMed).
: "Role of Ly-6 in lymphocyte activation. I. Characterization of a monoclonal antibody to a nonpolymorphic Ly-6 specificity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 10, pp. 3240-6, (1986) (PubMed).
: "Ly-6A.2 and Ly-6E.1 molecules are antithetical and identical to MALA-1." in: Immunogenetics, Vol. 23, Issue 3, pp. 197-207, (1986) (PubMed).
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: "In vitro expansion of hematopoietic progenitors and maintenance of stem cells: comparison between FLT3/FLK-2 ligand and KIT ligand." in: Blood, Vol. 89, Issue 6, pp. 1915-21, (1997) (PubMed).
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- 抗原
- Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))
- 别名
- Ly-6A/E (Ly6a 产品)
- 背景
- Synonyms: Sca-1
- 途径
- Sensory Perception of Sound, Activated T Cell Proliferation
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