ENPEP 抗体 (Biotin)
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北京 101111
Quick Overview for ENPEP 抗体 (Biotin) (ABIN2689818)
抗原
See all ENPEP 抗体适用
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产品特性
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The 6C3 antibody reacts with an epitope of the 6C3/BP-1 (Ly-51) glycoprotein cell-surface differentiation antigen, which was originally identified on pre-B cell lymphomas (spontaneous and chemical- or retrovirus- transformed, in vitro and in vivo). 6C3/BP-1 is a homodimer cell-surface glycoprotein with 140- kDa subunits which has been identified to possess aminopeptidase A (APA) activity. The same antigen is expressed at high levels on bone marrow stromal cell lines which support in vitro B lymphopoieses, on thymic dendritic cells and cortical epithelial cells, and on a wide variety of mouse and rat tissues known to possess APA activity. Subsets of normal bone marrow pre-B and B lymphocytes express low levels of Ly-51, which is rapidly up-regulated on the pre-B cells in the presence of IL-7. A role for the 6C3/BP-1 Molecule in the IL-7-driven proliferation of B cell precursors has been postulated. However, B-cell abnormalities were not detected in Ly-51-deficient mice. Mature B lymphocytes, thymocytes, peripheral T lymphocytes, erythroid cells, and myeloid cells (with the exception of thymic dendritic cells) do not express Ly-51. The 6C3 antibody can be used to identify cortical epithelium in frozen sections of thymuses from normal, SCID, and TCR-transgenic mice. It is possible that the low level of 6C3/BP-1 antigen detected, by flow cytometry, on some thymocytes may be passively adsorbed from adjacent epithelial cells during preparation of the cell suspensions.
BD Pharmingen™ Biotin Rat Anti-Mouse Ly-51 - Biotin - Clone 6C3 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- C57L mouse Pre-B lymphhoma cell line L1-2
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亚型
- IgG2a kappa
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anti-Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP) (AA 718-948) antibody
ENPEP 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP) antibodyENPEP 适用: 人, 小鼠, 猴 WB, IHC, IHC (p), IF 宿主: 小鼠 Monoclonal 1D4 unconjugated
anti-Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP) (AA 409-624) antibodyENPEP 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP) (AA 708-957) antibodyENPEP 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP) antibodyENPEP 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP) antibodyENPEP 适用: 人 WB, FACS, IF 宿主: 小鼠 Monoclonal 1D6 unconjugated
anti-Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP) (AA 719-949) antibodyENPEP 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP) (Internal Region) antibodyVerified ENPEP 适用: 人, 大鼠 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP) (C-Term) antibodyENPEP 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP) (AA 899-930), (C-Term) antibodyENPEP 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB19291 unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "T and B cell development in BP-1/6C3/aminopeptidase A-deficient mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 10, pp. 4681-7, (1998) (PubMed).
: "TCR signaling regulates thymic organization: lessons from TCR-transgenic mice." in: Immunology today, Vol. 18, Issue 5, pp. 204-8, (1997) (PubMed).
: "Regulation of B cell precursor proliferation by aminopeptidase A." in: International immunology, Vol. 7, Issue 5, pp. 737-46, (1995) (PubMed).
: "Mouse thymus dendritic cells: kinetics of development and changes in surface markers during maturation." in: European journal of immunology, Vol. 25, Issue 2, pp. 418-25, (1995) (PubMed).
: "Histidine residue in the zinc-binding motif of aminopeptidase A is critical for enzymatic activity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 4, pp. 1222-6, (1993) (PubMed).
: "Growth of epithelial cells in the thymic medulla is under the control of mature T cells." in: The Journal of experimental medicine, Vol. 176, Issue 2, pp. 611-6, (1992) (PubMed).
: "Bone marrow stromal cells and interleukin-7 induce coordinate expression of the BP-1/6C3 antigen and pre-B cell growth." in: International immunology, Vol. 2, Issue 8, pp. 697-705, (1991) (PubMed).
: "Aminopeptidase A activity of the murine B-lymphocyte differentiation antigen BP-1/6C3." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 2, pp. 676-80, (1991) (PubMed).
: "Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow." in: The Journal of experimental medicine, Vol. 173, Issue 5, pp. 1213-25, (1991) (PubMed).
: "The growth factor IL-7 induces expression of a transformation-associated antigen in normal pre-B cells." in: International immunology, Vol. 2, Issue 5, pp. 399-406, (1991) (PubMed).
: "BP-1/6C3 expression defines a differentiation stage of transformed pre-B cells and is not related to malignant potential." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 5, pp. 1603-8, (1990) (PubMed).
: "Molecular cloning of the murine BP-1/6C3 antigen: a member of the zinc-dependent metallopeptidase family." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 3, pp. 993-7, (1990) (PubMed).
: "The early B lineage antigen BP-1 and the transformation-associated antigen 6C3 are on the same molecule." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 143, Issue 10, pp. 3303-8, (1989) (PubMed).
: "Normal thymic cortical epithelial cells developmentally regulate the expression of a B-lineage transformation-associated antigen." in: Immunogenetics, Vol. 27, Issue 3, pp. 180-6, (1988) (PubMed).
: "Expression of the 6C3 antigen on murine hematopoietic neoplasms. Association with expression of abl, ras, fes, src, erbB, and Cas NS-1 oncogenes but not with myc." in: The Journal of experimental medicine, Vol. 165, Issue 3, pp. 920-5, (1987) (PubMed).
: "Transformation-associated proteins in murine B-cell lymphomas that are distinct from Abelson virus gene products." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 81, Issue 14, pp. 4434-8, (1984) (PubMed).
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: "T and B cell development in BP-1/6C3/aminopeptidase A-deficient mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 10, pp. 4681-7, (1998) (PubMed).
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- ENPEP (Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP))
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别名
- Ly-51
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物质类
- Chemical
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背景
- Synonyms: 6C3/BP-1 Antigen
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途径
- Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones
抗原
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