Ly-49C, Ly-49I 抗体 (Biotin)
Cited in 22+ publications. This Biotin-conjugated 小鼠 单克隆 anti-not_set antibody (Clone 5E6) specifically detects not_set in FACS 和 IP. The antibody is reactive with 小鼠 samples.
(22 references)
产品编号 ABIN2689814
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Quick Overview for Ly-49C, Ly-49I 抗体 (Biotin) (ABIN2689814)
抗原
Ly-49C, Ly-49I
适用
小鼠
宿主
小鼠
克隆类型
单克隆
标记
Biotin
应用范围
Flow Cytometry (FACS), Immunoprecipitation (IP)
克隆位点
5E6
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产品特性
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The 5E6 (also known as clone SW5E6) antibody reacts with Ly-49C[BALB], Ly-49C[B6], Ly-49C[NZB], and Ly-49I[B6], inhibitory receptors which are expressed on subsets of natural killer (NK) cells and NK-1.1+ (or DX5+) T lymphocytes (NK-T cells) in all strains tested except C57BR and RIII, on a population of memory CD8+ T lymphocytes and NK1.1+ γδ T cells in C57BL/6 mice, and on a distinct subset of B-1 cells of BALB/c and C57BL/6 mice. The proportion of NK T cells expressing Ly-49C/I is higher (2-5 fold) in thymus than in liver (immature and mature NK T cells, respectively), and there is evidence that the down-regulation of Ly-49 receptor expression is necessary for normal NK T-cell development. Most NK cells express a single allele of Ly-49C, although occasionally they may express more than one allele. The Ly-49 family of NK-cell receptors are disulfide-linked type-II transmembrane protein homodimers with extracellular carbohydrate-recognition domains (CRD) that bind to MHC class I alloantigens. The Ly-49 family members are expressed independently, such that an individual NK or T cell may display more than one class of Ly-49 receptor homodimers. The 5E6 antibody is specific for the Ly-49C CRD. The Ly-49C[BALB] and Ly-49C[B6] alloantigens bind to MHC class I antigens of the b, d, k, and s haplotypes, and the 5E6 antibody blocks this binding. Binding of Ly-49C[BALB]- and Ly-49C[B6]- expressing transfectants to lymphoblasts of H-2[f], H-2[q], H-2[r], and H-2[v] strains has also been detected. Ly-49I[B6] transfectants bind H-2[r] lymphoblasts and bind much more weakly to the b, d, k, q, s, and v haplotypes. The levels of the Ly-49 inhibitory receptors are down-regulated by their ligands in vivo, and the various levels of expression of an Ly-49 inhibitory receptor may affect the specificity of NK cells. Ly-49C is specifically downregulated in the presence of H-2K[b] class I molecules (one of the Ly-49C ligands). Ly-49C[+] and/or Ly-49I[+] cells mediate allogeneic and hybrid resistance to H-2d bone marrow transplantation. In vitro and in vivo studies suggest that the Ly-49C and/or Ly-49I receptors mediate negative regulation of NK-cell cytolytic activity via tyrosine phosphorylation of their ITIMs (Immunoreceptor Tyrosine-based Inhibitory Motifs). The epitope recognized by this antibody on Ly49C may be masked on freshly isolated primary NK cells due to cis interactions with MHC class I molecules. This observation has been reported for other Ly49C monoclonal antibodies that bind to the same structural region. Two-color analysis of the expression of Ly-49C/I on splenic NK cells. C57BL/6 splenocytes were simultaneously stained with PE-conjugated anti-mouse CD49b/Pan-NK Cells mAb DX5 (Cat. No. 553858) and either biotinylated mouse IgG2a, κ isotype control mAb G155-178 (Cat. No. 553455, left panel) or biotinylated mAb 5E6 (right panel), followed by Avidin-FITC (Cat. No. 554057). Flow cytometry was performed on a BD FACScan™ flow cytometry system. 557418 Rev. 15 Page 1 of 2
BD Pharmingen™ Biotin Mouse Anti-Mouse Ly-49C and Ly-49I - Biotin - Clone 5E6 - Isotype Mouse IgG2a, κ - Reactivity Ms - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Activated mouse NK cells
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亚型
- IgG2a kappa
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Interactions of Ly49 family receptors with MHC class I ligands in trans and cis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 3, pp. 1277-84, (2007) (PubMed).
: "Thymus-dependent modulation of Ly49 inhibitory receptor expression on NK1.1+gamma/delta T cells." in: Immunology, Vol. 102, Issue 1, pp. 24-30, (2001) (PubMed).
: "Memory CD8 T lymphocytes express inhibitory MHC-specific Ly49 receptors." in: European journal of immunology, Vol. 30, Issue 1, pp. 236-44, (2000) (PubMed).
: "Negative regulation of B cell receptor-mediated signaling in B-1 cells through CD5 and Ly49 co-receptors via Lyn kinase activity." in: International immunology, Vol. 12, Issue 10, pp. 1417-23, (2000) (PubMed).
: "Differential regulation of Ly49 expression on CD4+ and CD4-CD8- (double negative) NK1.1+ T cells." in: European journal of immunology, Vol. 30, Issue 9, pp. 2488-96, (2000) (PubMed).
: "Direct assessment of MHC class I binding by seven Ly49 inhibitory NK cell receptors." in: Immunity, Vol. 11, Issue 1, pp. 67-77, (1999) (PubMed).
: "The Ly-49 family: regulation of cytotoxicity and cytokine production in murine CD3+ cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 3, pp. 1158-65, (1998) (PubMed).
: "Differential effects of the rejection of bone marrow allografts by the depletion of activating versus inhibiting Ly-49 natural killer cell subsets." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 1, pp. 87-94, (1998) (PubMed).
: "Developmentally regulated extinction of Ly-49 receptor expression permits maturation and selection of NK1.1+ T cells." in: The Journal of experimental medicine, Vol. 187, Issue 12, pp. 2109-14, (1998) (PubMed).
: "Fine tuning of natural killer cell specificity and maintenance of self tolerance in MHC class I-deficient mice." in: European journal of immunology, Vol. 28, Issue 4, pp. 1315-21, (1998) (PubMed).
: "Heterogeneity among Ly-49C natural killer (NK) cells: characterization of highly related receptors with differing functions and expression patterns." in: The Journal of experimental medicine, Vol. 184, Issue 6, pp. 2085-90, (1997) (PubMed).
: "Host MHC class I gene control of NK-cell specificity in the mouse." in: Immunological reviews, Vol. 155, pp. 11-28, (1997) (PubMed).
: "Allorecognition by murine natural killer cells: studies with bone marrow transplants and lysis of T lymphoblasts." in: Chemical immunology, Vol. 64, pp. 164-80, (1997) (PubMed).
: "Differential tyrosine phosphorylation of inhibitory versus activating Ly-49 receptor proteins and their recruitment of SHP-1 phosphatase." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 9, pp. 4187-96, (1997) (PubMed).
: "NK cell receptor calibration: effects of MHC class I induction on killing by Ly49Ahigh and Ly49Alow NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 7, pp. 3189-94, (1997) (PubMed).
: "Specificity, tolerance and developmental regulation of natural killer cells defined by expression of class I-specific Ly49 receptors." in: Immunological reviews, Vol. 155, pp. 41-52, (1997) (PubMed).
: "Ly-49 G2+ NK cells are responsible for mediating the rejection of H-2b bone marrow allografts in mice." in: International immunology, Vol. 8, Issue 12, pp. 1833-9, (1997) (PubMed).
: "The Ly-49 family: genes, proteins and recognition of class I MHC." in: Immunological reviews, Vol. 155, pp. 67-77, (1997) (PubMed).
: "Recognition of class I major histocompatibility complex molecules by Ly-49: specificities and domain interactions." in: The Journal of experimental medicine, Vol. 183, Issue 4, pp. 1553-9, (1996) (PubMed).
: "The role of Ly49A and 5E6(Ly49C) molecules in hybrid resistance mediated by murine natural killer cells against normal T cell blasts." in: Immunity, Vol. 4, Issue 1, pp. 67-76, (1996) (PubMed).
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: "Interactions of Ly49 family receptors with MHC class I ligands in trans and cis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 3, pp. 1277-84, (2007) (PubMed).
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- Ly-49C, Ly-49I
抗原
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