Lgr5 CRL Region 抗体
This 大鼠 单克隆 antibody specifically detects Lgr5 CRL Region in BI, FACS, IF 和 WB. It exhibits reactivity toward 人. It has been mentioned in 8+ publications
(8 references)
产品编号 ABIN2689808
发货至:
中国
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Quick Overview for Lgr5 CRL Region 抗体 (ABIN2689808)
抗原
Lgr5 CRL Region
适用
人
宿主
大鼠
克隆类型
单克隆
应用范围
BioImaging (BI), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
克隆位点
1D9A5
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交叉反应 （详细）
- Mouse (Lack of Reactivity Confirmed in Development)
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产品特性
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Lgr5 (leucine-rich-repeat-containing G-protein-coupled receptor 5) is a seven transmembrane-domain receptor that is a target gene for Wnt and marks stem cells in the small intestine and colon, stomach, and hair follicle. Lgr5 was initially identified as a potential stem cell marker due to restricted expression of Lgr5 in the intestinal crypt and labeling of rapidly cycling cells of the colon and intestine. Using both lineage tracing and organiod culture experiments, Lgr5 positive cells are capable of generating all types of the small intestine epithelium hence indicating that Lgr5 marks stem cells of the small intestine and colon. R-spondin growth factors, which are secreted agonists of the Wnt pathway, bind Lgr5. The binding of R-spondins to Lgr5 leads to recruitment of the Frizzled/LRP Wnt receptor complex, which binds to Wnt ligands and leads to downstream Wnt signaling. Lgr5 is up-regulated in colon and ovarian cancers and has been implicated in promotion of tumor growth and metastasis. The 1D9A5 monoclonal antibody recognizes an epitope in the Cysteine-rich linker (CRL) region of Human Lgr5. Western blot analysis of human Lgr5. Lysate from colorectal adenocarcinoma cells LS 174T transfected with human LGR5 (Cells from Dr. Hans Clevers, Hubrecht Institute) was probed with Purified Rat anti-Human Lgr5 (CRL Region) monoclonal antibody (Cat. No. 562733) at titrations of 1.0 (lane 1), 0.5 (lane 2), and 0.25 μg/mL (lane 3). Lgr5 is identified as a strong band of ~110 kDa. The protein was detected using HRP Goat Anti-Rat Ig (Cat. No. 554017) and a chemiluminescent detection system. Immunofluorescent staining of human Lgr5. Colorectal adenocarcinoma cells LS 174T transfected with human LGR5 (Cells from Dr. Hans Clevers, Hubrecht Institute) were stained with Purified Rat anti-Human Lgr5 (CRL Region) monoclonal antibody (Cat. No. 562733) (pseudo colored green) at 5 μg/mL. The second-step reagent was Alexa Fluor® 488 goat anti-Rat IgG (Life Technologies). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ Software.
BD Pharmingen™ Purified Rat anti-Human Lgr5 (CRL Region) - Purified - Clone 1D9A5 (also known as 1D9) - Isotype Rat IgG2b, λ - Reactivity Hu, Ms - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Human LGR5 DNA
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亚型
- IgG2b lambda
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应用备注
- Immunohistochemistry-frozen, Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Monoclonal antibodies against Lgr5 identify human colorectal cancer stem cells." in: Stem cells (Dayton, Ohio), Vol. 30, Issue 11, pp. 2378-86, (2012) (PubMed).
: "Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5⁺ stem cell." in: Nature medicine, Vol. 18, Issue 4, pp. 618-23, (2012) (PubMed).
: "R-spondins function as ligands of the orphan receptors LGR4 and LGR5 to regulate Wnt/beta-catenin signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 28, pp. 11452-7, (2011) (PubMed).
: "The intestinal stem cell signature identifies colorectal cancer stem cells and predicts disease relapse." in: Cell stem cell, Vol. 8, Issue 5, pp. 511-24, (2011) (PubMed).
: "Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling." in: Nature, Vol. 476, Issue 7360, pp. 293-7, (2011) (PubMed).
: "Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro." in: Cell stem cell, Vol. 6, Issue 1, pp. 25-36, (2010) (PubMed).
: "Lgr5 marks cycling, yet long-lived, hair follicle stem cells." in: Nature genetics, Vol. 40, Issue 11, pp. 1291-9, (2008) (PubMed).
: "Identification of stem cells in small intestine and colon by marker gene Lgr5." in: Nature, Vol. 449, Issue 7165, pp. 1003-7, (2007) (PubMed).
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- Lgr5 CRL Region
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背景
- Synonyms: GPR49, GPR67, HG38
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分子量
- ~110 kDa
抗原
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