Lgr5 Central LRR 抗体
There are 7+ publications for this product available. The 大鼠 单克隆 anti-Lgr5 Central LRR antibody is suitable to detect Lgr5 Central LRR in samples from 人. It has been validated for BI, FACS 和 IF.
(7 references)
产品编号 ABIN2689806
发货至:
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Quick Overview for Lgr5 Central LRR 抗体 (ABIN2689806)
抗原
Lgr5 Central LRR
适用
人
宿主
大鼠
克隆类型
单克隆
应用范围
BioImaging (BI), Flow Cytometry (FACS), Immunofluorescence (IF)
克隆位点
4D11F8
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交叉反应 （详细）
- Mouse (Lack of Reactivity Confirmed in Development)
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产品特性
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Lgr5 (leucine-rich-repeat-containing G-protein-coupled receptor 5) is a seven transmembrane-domain receptor that is a target gene for Wnt and marks stem cells in the small intestine, colon, stomach, and hair follicle. Lgr5 was initially identified as a potential stem cell marker due to restricted expression of Lgr5 in the intestinal crypt and labeling of rapidly cycling cells of the colon and intestine. Using both lineage tracing and organoid culture experiments, Lgr5 positive cells are capable of generating all types of the small intestine epithelium hence indicating that Lgr5 marks stem cells of the small intestine and colon. R-spondin growth factors, which are secreted agonists of the Wnt pathway, bind Lgr5. The binding of R-spondins to Lgr5 leads to recruitment of the Frizzled/LRP Wnt receptor complex, which binds to Wnt ligands and leads to downstream Wnt signaling. Lgr5 is up-regulated in colon and ovarian cancers and has been implicated in promotion of tumor growth and metastasis. The 4D11F8 monoclonal antibody recognizes an epitope in the center of the leucine-rich repeat (LRR) region of Human Lgr5. Flow cytometric analysis of human LGR5. Colorectal adenocarcinoma cells LS 174T (ATCC CL-188) were stained with either Purified Rat IgG2b isotype control (Cat. No. 553986) or Purified Rat anti-human LGR5 (Central LRR) monoclonal antibody (solid line) at matched concentrations. Cells were harvested with Accutase™ Cell Detachment Solution (Cat. No. 561527).The second step reagent was PE goat anti-Rat Ig (Cat. No. 550767). The histograms were derived from gated events based on light scattering characteristics of the LS 174T cells. Flow cytometry was performed on a BD FACSCanto™ II flow cytometry system. Immunoflourescent staining of human LGR5. Colorectal adenocarcinoma cells LS 174T transfected with human LGR5 (Cells from Dr. Hans Clevers, Hubrecht Institute) were fixed and stained with Purified Rat anti-human LGR5 (Central LRR) monoclonal antibody (pseudo colored green) at 2.5 μg/mL. The second-step reagent was Alexa Fluor® 488 goat anti-rat Ig (Life Technologies) and counter-staining was with DAPI (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ Software. 562732 Rev. 2 Page 1 of 2
BD Pharmingen™ Purified Rat anti-Human Lgr5 (Central LRR) - Purified - Clone 4D11F8 (also known as 4D11) - Isotype Rat IgG2b, λ - Reactivity Hu, Ms - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Human LGR5 DNA
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亚型
- IgG2b lambda
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应用备注
- Western blot, Immunohistochemistry-frozen, Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5⁺ stem cell." in: Nature medicine, Vol. 18, Issue 4, pp. 618-23, (2012) (PubMed).
: "R-spondins function as ligands of the orphan receptors LGR4 and LGR5 to regulate Wnt/beta-catenin signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 28, pp. 11452-7, (2011) (PubMed).
: "The intestinal stem cell signature identifies colorectal cancer stem cells and predicts disease relapse." in: Cell stem cell, Vol. 8, Issue 5, pp. 511-24, (2011) (PubMed).
: "Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling." in: Nature, Vol. 476, Issue 7360, pp. 293-7, (2011) (PubMed).
: "Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro." in: Cell stem cell, Vol. 6, Issue 1, pp. 25-36, (2010) (PubMed).
: "Lgr5 marks cycling, yet long-lived, hair follicle stem cells." in: Nature genetics, Vol. 40, Issue 11, pp. 1291-9, (2008) (PubMed).
: "Identification of stem cells in small intestine and colon by marker gene Lgr5." in: Nature, Vol. 449, Issue 7165, pp. 1003-7, (2007) (PubMed).
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- Lgr5 Central LRR
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背景
- Synonyms: GPR49, GPR67, HG38
抗原
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