IL-27 抗体
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Quick Overview for IL-27 抗体 (ABIN2689756)
抗原
See all IL-27 (IL27) 抗体适用
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克隆类型
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应用范围
克隆位点
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产品特性
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The MM27.7B1 monoclonal antibody specifically binds to the p28 subunit of mouse Interleukin-27 (IL-27 p28) that is also known as IL-27 alpha (IL-27α) or Interleukin-30 (IL-30). IL-27 p28 is a member of the IL-6/IL-12 family of cytokines. The IL-27 p28 subunit binds to the EBI3 (Epstein-Barr virus induced gene 3) protein to form heterodimeric Interleukin-27. IL-27 p28 is produced by macrophages and dendritic cells stimulated by inflammatory cytokines and ligands for Toll-like receptors (TLR). IL-27 binds to and transduces signal through surface IL-27 receptors (IL-27R) that are expressed by B cells and naive T cells and more highly expressed by NK cells and activated T cells. The IL-27R complex is comprised of gp130 (CD130) and WSX-1 (IL-27R alpha) subunits. IL-27 signals through IL-27R that activate the Jak/STAT signaling pathway resulting in the phosphorylation of Stat1 and Stat3. It acts in a proinflammatory manner to upregulate cellular T-bet transcription factor and surface IL-12R beta2 expression contributing to higher levels of IFN-γ and granzyme B expression. IL-27 exerts anti-inflammatory responses by suppressing the differentiation and proliferation of Th2 and Th17 cells. IL-27 p28 is reportedly produced and secreted independently of EBI3. It may inhibit the actions of IL-17 and other proinflammatory cytokines like IL-6. The MM27/7B1 antibody has been reported to neutralize IL-27 activity in vitro. Routine quality testing of mAb MM27.7B1 is performed via flow cytometry of intracellularly stained IL-27 p28-transfected cells.
BD Pharmingen™ Purified NA/LE Mouse Anti-Mouse IL-27 p28 - NA/LE - Clone MM27.7B1 - Isotype Mouse IgG2a, κ - Reactivity Ms - 0.5 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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过滤
- Sterile filtered,0.2 μm filtered
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内毒素水平
- Endotoxin level is ≤0.01 EU/μg (≤0.001 ng/μg) of protein as determined by the LAL assay.
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免疫原
- Mouse IL-27
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亚型
- IgG2a kappa
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anti-Interleukin 27 (IL27) (AA 177-243) antibody
IL27 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 3F12 unconjugated
anti-Interleukin 27 (IL27) (C-Term) antibodyIL27 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 27 (IL27) (AA 1-243) antibodyIL27 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 27 (IL27) (AA 1-243) antibodyIL27 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Interleukin 27 (IL27) (N-Term) antibodyIL27 适用: 人, 小鼠 WB, IHC, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 27 (IL27) (AA 57-87) antibodyIL27 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB3263 unconjugated
anti-Interleukin 27 (IL27) antibodyIL27 适用: 小鼠 WB, ELISA, FACS, MA 宿主: 大鼠 Monoclonal 3H12-F10 unconjugated
anti-Interleukin 27 (IL27) (AA 29-243) antibodyIL27 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 27 (IL27) (AA 31-46) antibodyIL27 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 27 (IL27) antibodyIL27 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal B-G49 unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
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储存液
- Without preservative
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注意事项
- This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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: "The immunobiology of IL-27." in: Advances in immunology, Vol. 115, pp. 1-44, (2012) (PubMed).
: "A role for IL-27p28 as an antagonist of gp130-mediated signaling." in: Nature immunology, Vol. 11, Issue 12, pp. 1119-26, (2010) (PubMed).
: "Advances in understanding the anti-inflammatory properties of IL-27." in: Immunology letters, Vol. 117, Issue 2, pp. 123-30, (2008) (PubMed).
: "Augmentation of effector CD8+ T cell generation with enhanced granzyme B expression by IL-27." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 3, pp. 1686-93, (2005) (PubMed).
: "Induction of IgG2a class switching in B cells by IL-27." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 4, pp. 2479-85, (2004) (PubMed).
: "IL-27 and IFN-alpha signal via Stat1 and Stat3 and induce T-Bet and IL-12Rbeta2 in naive T cells." in: Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, Vol. 23, Issue 9, pp. 513-22, (2003) (PubMed).
: "IL-27, a heterodimeric cytokine composed of EBI3 and p28 protein, induces proliferation of naive CD4+ T cells." in: Immunity, Vol. 16, Issue 6, pp. 779-90, (2002) (PubMed).
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: "The immunobiology of IL-27." in: Advances in immunology, Vol. 115, pp. 1-44, (2012) (PubMed).
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- IL-27 (IL27) (Interleukin 27 (IL27))
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别名
- IL-27 p28
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背景
- Synonyms: Il27, IL-27 alpha, Il27a, IL27-A, IL-27-A, IL-27α, IL-27 p28, Il30, IL-30
抗原
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