(3 references)-
- 抗原 See all IL-2 (IL2) 抗体
- IL-2 (IL2) (Interleukin 2 (IL2))
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适用
- 人
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This IL-2 antibody is un-conjugated
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应用范围
- ELISA, Blocking Antibody (Inhibition)
- 品牌
- BD Pharmingen™
- 产品特性
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The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2). The immunogen used to generate the MQ1-17H12 hybridoma was recombinant human IL-2. Unconjugated or purified forms of this antibody have been reported to be neutralizing for human IL-2 bioactivity. Expression of IL-2 by stimulated CD3+ human PBMC. Human PBMCs were stimulated for 6 hours with PMA (50 ng/mL final concentration, Sigma, Cat. #P-8139) and calcium ionophore A23187 (500-100 ng/mL final concentration, Sigma, Cat. #C-9275) in the presence of BD GolgiStop™ (2 μM final concentration, Cat. No. 554724). The PBMCs were stained with PE-Cy™5-anti-CD3 (PE-Cy™5-UCHT1, Cat. No. 555334), fixed, permeabilized, and then stained with 0.25 μg of FITC-rat anti-human IL-2 antibody (FITC-MQ1-17H12, Cat. No. 554565) using Pharmingen's staining protocol (left panel). To demonstrate specificity of staining, the binding of FITC-MQ1-17H12 was blocked by the preincubation of the fluorochrome - conjugated antibody with recombinant human IL-2 (10 ng/mL final concentration, Cat. No. 554603, middle panel), and by preincubation of the fixed/permeabilized cells with unlabeled MQ1-17H12 antibody (5.0 μg, Cat. No. 554563, right panel) prior to staining with the FITC-MQ1-17H12 antibody. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking (middle panel) and unlabeled antibody blocking (right panel) specificity controls.
BD Pharmingen™ Purified Rat Anti-Human IL-2 - Purified - Clone MQ1-17H12 - Isotype Rat IgG2a, κ - Reactivity Hu - 0.1 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human IL-2 Recombinant Protein
- 克隆位点
- MQ1
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product IL2 Primary Antibody
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anti-Interleukin 2 (IL2) (AA 21-153) antibody
IL2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 (IL2) (AA 21-153) antibodyIL2 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C7 unconjugated
anti-Interleukin 2 (IL2) (AA 10-169) antibodyIL2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 (IL2) (AA 21-153) antibodyIL2 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C5 unconjugated
anti-Interleukin 2 (IL2) antibodyIL2 适用: 人, 小鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 (IL2) antibodyIL2 适用: 人 WB, ELISA, FACS, IP, ICC 宿主: 小鼠 Monoclonal 35C3 unconjugated
anti-Interleukin 2 (IL2) antibody (APC)IL2 适用: 人 FACS 宿主: 小鼠 Monoclonal 35C3 APC
anti-Interleukin 2 (IL2) antibody (PE)IL2 适用: 人 FACS 宿主: 小鼠 Monoclonal 35C3 PE
anti-Interleukin 2 (IL2) (AA 21-153) antibodyIL2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 (IL2) antibodyIL2 适用: 人 WB, ELISA, FACS 宿主: 兔 Monoclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).
: "Concomitant in vivo production of 19 different cytokines in human tonsils." in: Immunology, Vol. 83, Issue 1, pp. 16-24, (1995) (PubMed).
: "Cytokine synthesis analyzed at the single-cell level before and after revaccination with tetanus toxoid." in: European journal of immunology, Vol. 24, Issue 8, pp. 1808-15, (1994) (PubMed).
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: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).
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- 抗原
- IL-2 (IL2) (Interleukin 2 (IL2))
- 别名
- IL-2 (IL2 产品)
- 别名
- IL2 antibody, IL-2 antibody, il2 antibody, TCGF antibody, lymphokine antibody, Il-2 antibody, POIL2 antibody, interleukin 2 antibody, interleukin-15 antibody, IL2 antibody, il2 antibody, LOC100303688 antibody, Il2 antibody
- 途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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