MHC Class II I-Ek 抗体
(8 references)-
- 抗原 See all MHC Class II I-Ek products
- MHC Class II I-Ek
- 适用
- 小鼠, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This MHC Class II I-Ek antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Cytotoxicity Test (CyTox), Blocking Reagent (BR)
- 品牌
- BD Pharmingen™
- 产品特性
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The 14-4-4S antibody reacts with the I-E[k] MHC class II alloantigen. It cross-reacts with cells from mice of the H-2[d], H-2[p], and H-2[r] haplotypes. Cells from mice of the H-2[b], H-2[f], H-2[g7], H-2[q], and H-2[s] haplotypes do not express I-E antigen. It has been reported that mAb 14-4-4S cross-reacts with the rat MHC class II antigen RT1D. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.
BD Pharmingen™ Purified Mouse Anti-Mouse I-E[k] - Purified - Clone 14-4-4S - Isotype Mouse IgG2a, κ - Reactivity Ms, Rat - 0.1 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- C3H mouse skin graft and splenocytes
- 克隆位点
- 14-4
- 亚型
- IgG2a kappa
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anti-MHC Class II I-Ek antibody (PE)
适用: 小鼠 FACS 宿主: 小鼠 Monoclonal 17-3-3S PE
anti-MHC Class II I-Ek antibody适用: 小鼠 FACS 宿主: 小鼠 Monoclonal 17-3-3S unconjugated
anti-MHC Class II I-Ek antibody (Biotin)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal 17-3-3S Biotin
anti-MHC Class II I-Ek antibody (FITC)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal 17-3-3S FITC
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "The immunological synapse: a molecular machine controlling T cell activation." in: Science (New York, N.Y.), Vol. 285, Issue 5425, pp. 221-7, (1999) (PubMed).
: "Studies on the interdependence of gp39 and B7 expression and function during antigen-specific immune responses." in: European journal of immunology, Vol. 25, Issue 2, pp. 596-603, (1995) (PubMed).
: "Non-coordinate synthesis of MHC class II proteins and invariant chains by epidermal Langerhans cells derived from short-term in vitro culture." in: International immunology, Vol. 6, Issue 1, pp. 61-71, (1994) (PubMed).
: "Structure function analysis of the H-2 Abp gene." in: Immunogenetics, Vol. 34, Issue 6, pp. 358-65, (1992) (PubMed).
: "The NOD mouse: recessive diabetogenic gene in the major histocompatibility complex." in: Science (New York, N.Y.), Vol. 231, Issue 4739, pp. 733-5, (1986) (PubMed).
: "Biochemical characterization of Ia antigens encoded by the RT1.B and RT1.D loci in the rat MHC." in: Immunogenetics, Vol. 17, Issue 5, pp. 475-84, (1983) (PubMed).
: "A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 127, Issue 6, pp. 2488-95, (1982) (PubMed).
: "Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 124, Issue 2, pp. 533-40, (1980) (PubMed).
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: "The immunological synapse: a molecular machine controlling T cell activation." in: Science (New York, N.Y.), Vol. 285, Issue 5425, pp. 221-7, (1999) (PubMed).
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- 抗原
- MHC Class II I-Ek
- 别名
- I-E[k] (MHC Class II I-Ek 产品)
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