MHC II (I-A/I-E) 抗体
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北京 101111
Quick Overview for MHC II (I-A/I-E) 抗体 (ABIN2689576)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
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The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-A[b], I-A[d], I-A[q], I-E[d], and I-E[k] (but not I-A[f], I-A[k], or I-A[s]) MHC class II alloantigens. It also reacts with cells from mice of the H-2[p] and H-2[r] haplotypes, and it is non-reactive with cells from NOD (H-2[g7]) mice. Flow cytometric analysis indicates that the M5/114.15.2 and 2G9 (Cat. No. 553621) monoclonal antibodies have comparable reactivity on cells from mice with I-A[b], I-A[d], I-A[g7], I-A[q], I-E[d], and I-E[k] alloantigens. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.
BD Pharmingen™ Purified Rat Anti-Mouse I-A/I-E - Purified - Clone M5/114.15.2 (also known as M5/114) - Isotype Rat IgG2b, κ - Reactivity Ms - 0.5 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Activated C57BL/6 Mouse Spleen
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亚型
- IgG2b kappa
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anti-MHC II (I-A/I-E) antibody (FITC)
适用: 小鼠 FACS 宿主: 大鼠 Monoclonal M5-114-15-2 FITC
anti-MHC II (I-A/I-E) antibody (APC)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal M5-114-15-2 APC
anti-MHC II (I-A/I-E) antibody (PE-Cy7)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal M5-114-15-2 PE-Cy7
anti-MHC II (I-A/I-E) antibody (violetFluor™ 450)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal M5-114-15-2 violetFluor™ 450
anti-MHC II (I-A/I-E) antibody (Biotin)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal M5-114-15-2 Biotin
anti-MHC II (I-A/I-E) antibody (violetFluor™ 500)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal M5-114-15-2 violetFluor™ 500
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Promiscuous presentation and recognition of nucleosomal autoepitopes in lupus: role of autoimmune T cell receptor alpha chain." in: The Journal of experimental medicine, Vol. 187, Issue 3, pp. 367-78, (1998) (PubMed).
: "Molecular structure and function of CD4 on murine egg plasma membrane." in: Zygote (Cambridge, England), Vol. 3, Issue 1, pp. 65-73, (1995) (PubMed).
: "CD69 cell surface expression identifies developing thymocytes which audition for T cell antigen receptor-mediated positive selection." in: International immunology, Vol. 5, Issue 9, pp. 1139-50, (1994) (PubMed).
: "Medullary thymic epithelium expresses a ligand for CTLA4 in situ and in vitro." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 5, pp. 2453-61, (1993) (PubMed).
: "Mice lacking the MHC class II-associated invariant chain." in: Cell, Vol. 72, Issue 4, pp. 635-48, (1993) (PubMed).
: "The NOD mouse: recessive diabetogenic gene in the major histocompatibility complex." in: Science (New York, N.Y.), Vol. 231, Issue 4739, pp. 733-5, (1986) (PubMed).
: "A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 127, Issue 6, pp. 2488-95, (1982) (PubMed).
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- MHC II (I-A/I-E)
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别名
- I-A/I-E
抗原
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