(5 references)-
- 抗原
- H2-M3 (Histocompatibility 2, M Region Locus 3 (H2-M3))
- 适用
- 化学剂
- 宿主
-
Armenian Hamster
- 克隆类型
- 单克隆
- 应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP)
- 品牌
- BD Pharmingen™
- 产品特性
-
The hamster anti-mouse H2-M3 antibody (clone 130) reacts with the H2-M3 major histocompatibility complex (MHC) non-classical class Ib antigen. H2-M3 (M3) associates with β2-microglobulin and is capable of being expressed by most leukocytes. However, due to a lack of endogenous antigens, M3 has been reported to be undetectable on most cells. Its expression is induced by high-affinity N-formylated peptides from mitochondria, Listeria monocytogenes, and Mycobacterium tuberculosis. The induced expression of M3 is most efficient on antigen presenting cells. M3 presents antigen to cytotoxic T lymphocytes and may play a key role in protective immunity against the intracellular bacteria L. monocytogenes and M. tuberculosis. Furthermore, M3 is capable of presenting mitochondrial antigens for intrathymic positive selection of T-cell receptors which recognize those peptides. Upregulation of H2-M3 expression by exogenous N-formylated peptide. C57BL/6 splenocytes were cultured overnight in the absence (left panel) or presence (right panel) of N-
BD Pharmingen™ Purified Hamster Anti-Mouse H2-M3 - Purified - Clone 130 - Isotype Armenian Hamster IgG - Reactivity Ms - 0.1 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Purified soluble complex of recombinant truncated M3 and beta2-microglobulin
- 克隆位点
- 130
- 亚型
- IgG
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
-
- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Induction of M3-restricted cytotoxic T lymphocyte responses by N-formylated peptides derived from Mycobacterium tuberculosis." in: The Journal of experimental medicine, Vol. 193, Issue 10, pp. 1213-20, (2001) (PubMed).
: "Positive selection of an H2-M3 restricted T cell receptor." in: Immunity, Vol. 11, Issue 1, pp. 33-43, (1999) (PubMed).
: "Response to Listeria monocytogenes in mice lacking MHC class Ia molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 9, pp. 5429-36, (1999) (PubMed).
: "H2-M3, a full-service class Ib histocompatibility antigen." in: Annual review of immunology, Vol. 15, pp. 851-79, (1997) (PubMed).
: "Antigen presentation by major histocompatibility complex class I-B molecules." in: Annual review of immunology, Vol. 12, pp. 839-80, (1994) (PubMed).
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: "Induction of M3-restricted cytotoxic T lymphocyte responses by N-formylated peptides derived from Mycobacterium tuberculosis." in: The Journal of experimental medicine, Vol. 193, Issue 10, pp. 1213-20, (2001) (PubMed).
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- 抗原
- H2-M3 (Histocompatibility 2, M Region Locus 3 (H2-M3))
- 别名
- H2-M3
- 物质类
- Chemical
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