MHC Class I (H-2Kb/H-2Db) 抗体 (Biotin)
Cited in 3+ publications. This Biotin-conjugated 小鼠 单克隆 anti-not_set antibody (Clone 28-8) specifically detects not_set in FACS. The antibody is reactive with 小鼠 samples.
(3 references)
产品编号 ABIN2689543
发货至:
中国
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Quick Overview for MHC Class I (H-2Kb/H-2Db) 抗体 (Biotin) (ABIN2689543)
抗原
MHC Class I (H-2Kb/H-2Db)
适用
小鼠
宿主
小鼠
克隆类型
单克隆
标记
Biotin
应用范围
Flow Cytometry (FACS)
克隆位点
28-8
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产品特性
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The 28-8-6 antibody reacts with the H-2K[b] and H-2D[b] MHC class I alloantigens. The reaction with H-2K[b] has been reported to be stronger than that with H-2D[b]. Its recognition of H-2D[b] is dependent upon the presence of β2 microglobulin. Cross-reactivity with an epitope on the N-terminal domains, α1 and α2, of the H-2D[d] MHC class I alloantigen has also been described. Reactivity with other haplotypes (e.g., f, k, p, q, r, s) has not been observed.
BD Pharmingen™ Biotin Mouse Anti-Mouse H-2K[b]/H-2D[b] - Biotin - Clone 28-8-6 - Isotype Mouse IgG2a, κ - Reactivity Ms - 0.5 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- C3H.SW mouse splenocytes
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亚型
- IgG2a kappa
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "Beta 2-microglobulin is not required for cell surface expression of the murine class I histocompatibility antigen H-2Db or of a truncated H-2Db." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 19, pp. 7447-51, (1986) (PubMed).
: "Exon shuffling: mapping polymorphic determinants on hybrid mouse transplantation antigens." in: Nature, Vol. 300, Issue 5894, pp. 755-7, (1983) (PubMed).
: "Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 1, pp. 317-21, (1981) (PubMed).
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: "Beta 2-microglobulin is not required for cell surface expression of the murine class I histocompatibility antigen H-2Db or of a truncated H-2Db." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 19, pp. 7447-51, (1986) (PubMed).
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- MHC Class I (H-2Kb/H-2Db)
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别名
- H-2K[b]/H-2D[b]
抗原
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