H-2Dd 抗体
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北京 101111
Quick Overview for H-2Dd 抗体 (ABIN2689537)
抗原
适用
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克隆类型
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应用范围
克隆位点
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产品特性
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The 34-5-8S antibody recognizes an epitope on the N-terminal domains, α1 and α2, of the H-2Dd. The mAb identifies a conformationally sensitive epitope of H-2Dd associated with β2 microglobulin, it fails to react with free H-2D[d] α chains synthesized in vitro. Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H-2s haplotypes has been observed by flow cytometric analysis. Reactivity with other haplotypes (e.g., f, k, p, r) has not been observed. mAb 34-5-8S has been reported to block the recognition of H-2Dd by Ly-49A+, C+, F+, G2+, or I+ NK cells or transfectants. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.
BD Pharmingen™ Purified Mouse Anti-Mouse H-2D[d] - Purified - Clone 34-5-8S - Isotype Mouse IgG2a, κ - Reactivity Ms - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- (C57BL/6 x DBA/2)F1 hybrid mouse splenocytes
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亚型
- IgG2a kappa
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anti-MHC Class I H-2Dd antibody (Biotin)
适用: 小鼠 FACS, IP 宿主: 小鼠 Monoclonal 34-5-8S Biotin
anti-MHC Class I H-2Dd antibody (PE)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal 34-5-8S PE
anti-MHC Class I H-2Dd antibody适用: 小鼠 FACS, IP 宿主: 小鼠 Monoclonal 34-5-8S unconjugated
anti-MHC Class I H-2Dd antibody (FITC)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal 34-5-8S FITC
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Direct assessment of MHC class I binding by seven Ly49 inhibitory NK cell receptors." in: Immunity, Vol. 11, Issue 1, pp. 67-77, (1999) (PubMed).
: "Evidence for sulfate modification of H-2Dd on N-linked carbohydrate(s): possible involvement in Ly-49A interaction." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 9, pp. 4367-74, (1998) (PubMed).
: "Recognition of class I major histocompatibility complex molecules by Ly-49: specificities and domain interactions." in: The Journal of experimental medicine, Vol. 183, Issue 4, pp. 1553-9, (1996) (PubMed).
: "Cloning and functional characteristics of murine large granular lymphocyte-1: a member of the Ly-49 gene family (Ly-49G2)" in: The Journal of experimental medicine, Vol. 182, Issue 2, pp. 293-303, (1995) (PubMed).
: "A natural killer cell receptor specific for a major histocompatibility complex class I molecule." in: The Journal of experimental medicine, Vol. 180, Issue 2, pp. 687-92, (1994) (PubMed).
: "Ly-49 mediates EL4 lymphoma adhesion to isolated class I major histocompatibility complex molecules." in: The Journal of experimental medicine, Vol. 179, Issue 3, pp. 1011-5, (1994) (PubMed).
: "Peptide and beta 2-microglobulin regulation of cell surface MHC class I conformation and expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 12, pp. 3723-32, (1992) (PubMed).
: "Exon shuffling: mapping polymorphic determinants on hybrid mouse transplantation antigens." in: Nature, Vol. 300, Issue 5894, pp. 755-7, (1983) (PubMed).
: "Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 1, pp. 317-21, (1981) (PubMed).
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- H-2Dd (MHC Class I H-2Dd)
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别名
- H-2D[d]
抗原
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