CXCR5 抗体
(6 references)Quick Overview for CXCR5 抗体 (ABIN2689483)
抗原
See all CXCR5 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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品牌
- BD Pharmingen™
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产品特性
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The monoclonal antibody 2G8 reacts with the mouse CXC chemokine receptor, CXCR5. CXCR5 (a.k.a. BLR1, NLR and MDR15), a seven-transmembrane, G-protein-coupled receptor, is the specific receptor for CXC chemokines, CXCL13/BLC/BCA-1. The expression of CXCR5 has been detected in spleen, lymph nodes, tonsils, brain, bone marrow, T cells, B cells, cerebrum, cerebellum, hippcampus and pituitary. In mouse spleen, CXCR5 was strictly expressed by mature B cells and a small subset of T lymphocytes. The immunogen used to generate 2G8 hybridoma was a recombinant protein containing N-terminal amino acids of mouse CXCR5 (GST-NmBLR1). This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Detection of CXCR5 expression on mouse splenocytes by purified 2G8. Mouse splenocytes were stained with 0.25/test μg of purified 2G8 using 3-step staining protocol below and anti-mouse CD45R/B220-FITC (Cat. No. 553088). The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed by using purified rat IgG2a (Cat. No. 553927) as isotype control. The quadrant markers for the bivariate dot plots were set based on the isotype control.
BD Pharmingen™ Purified Rat Anti-Mouse CXCR5 - Purified - Clone 2G8 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Mouse CXCR5
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亚型
- IgG2a kappa
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anti-Chemokine (C-X-C Motif) Receptor 5 (CXCR5) (AA 1-55) antibody
CXCR5 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 5 (CXCR5) antibodyCXCR5 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 5 (CXCR5) (AA 281-372) antibodyCXCR5 适用: 人, 小鼠 WB, ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 5 (CXCR5) (N-Term) antibodyCXCR5 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 5 (CXCR5) (AA 1-30), (N-Term) antibodyCXCR5 适用: 人 WB 宿主: 兔 Polyclonal RB41684 unconjugated
anti-Chemokine (C-X-C Motif) Receptor 5 (CXCR5) (Cytoplasmic Domain) antibodyCXCR5 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 5 (CXCR5) (AA 1-372) antibodyCXCR5 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 5 (CXCR5) (AA 1-55) antibodyCXCR5 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C1 unconjugated
anti-Chemokine (C-X-C Motif) Receptor 5 (CXCR5) (AA 23-38), (Extracellular) antibodyCXCR5 适用: 人 WB, FACS, IF, IC, LCI 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 5 (CXCR5) (N-Term) antibodyCXCR5 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "A B-cell-homing chemokine made in lymphoid follicles activates Burkitt's lymphoma receptor-1." in: Nature, Vol. 391, Issue 6669, pp. 799-803, (1998) (PubMed).
: "B cell-attracting chemokine 1, a human CXC chemokine expressed in lymphoid tissues, selectively attracts B lymphocytes via BLR1/CXCR5." in: The Journal of experimental medicine, Vol. 187, Issue 4, pp. 655-60, (1998) (PubMed).
: "A putative chemokine receptor, BLR1, directs B cell migration to defined lymphoid organs and specific anatomic compartments of the spleen." in: Cell, Vol. 87, Issue 6, pp. 1037-47, (1997) (PubMed).
: "Cloning of a novel putative G-protein-coupled receptor (NLR) which is expressed in neuronal and lymphatic tissue." in: FEBS letters, Vol. 321, Issue 2-3, pp. 173-8, (1993) (PubMed).
: "The G protein-coupled receptor BLR1 is involved in murine B cell differentiation and is also expressed in neuronal tissues." in: European journal of immunology, Vol. 23, Issue 10, pp. 2532-9, (1993) (PubMed).
: "Differentiation-specific expression of a novel G protein-coupled receptor from Burkitt's lymphoma." in: European journal of immunology, Vol. 22, Issue 11, pp. 2795-9, (1992) (PubMed).
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: "A B-cell-homing chemokine made in lymphoid follicles activates Burkitt's lymphoma receptor-1." in: Nature, Vol. 391, Issue 6669, pp. 799-803, (1998) (PubMed).
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- CXCR5 (Chemokine (C-X-C Motif) Receptor 5 (CXCR5))
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别名
- CXCR5
抗原
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