(5 references)-
- 抗原 See all CXCR5 抗体
- CXCR5 (Chemokine (C-X-C Motif) Receptor 5 (CXCR5))
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适用
- 人
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This CXCR5 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS)
- 品牌
- BD Pharmingen™
- 产品特性
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The monoclonal antibody RF8B2 reacts with the human CXC chemokine receptor, CXCR5. CXCR5 (aka BLR-1 NLR and MDR15), a seven transmembrane, G-protein-coupled receptor, is the specific receptor for CXC chemokine, CXCL13/BLC/BCA-1. In peripheral blood, CXCR5 expression is restricted to B lymphocytes and a small subset of CD4+ and CD8+ lymphocytes. The restricted expression pattern of CXCR5 on B cells suggested that this receptor might function as a regulator of B cell migration. Stimulation of T cells with anti-CD3 monoclonal antibody leads to the down-regulation of CXCR5. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Detection of CXCR5 expression on human peripheral lymphocytes by purified RF8B2. Human peripheral blood mononuclear cells were stained with 0.25 μg/test of purified RF8B2 using 3-step staining protocol outlined above and anti-human CD3-FITC (Cat. No. 555339, left panel) or anti-human CD19 FITC (Cat. No. 555412, right panel). The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed by using purified rat IgG2b (Cat. No. 555846 and FITC-conjugated mouse IgG2a (Cat. No. 555573) or FITC-conjugated mouse IgG1 (Cat. No. 555748) as isotype controls. The quadrant markers for the bivariate dot plots were set based on the isotype controls. Flow cytometry was performed using a BD FACScan™ Flow Cytometer
BD Pharmingen™ Purifed Rat Anti-Human CXCR5 - Purified - Clone RF8B2 - Isotype Rat IgG2b, κ - Reactivity Hu - 0.1 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human CXCR5
- 克隆位点
- RF8B2
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product CXCR5 Primary Antibody
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anti-Chemokine (C-X-C Motif) Receptor 5 (CXCR5) (AA 1-55) antibody
CXCR5 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 5 (CXCR5) antibodyCXCR5 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 5 (CXCR5) antibodyCXCR5 适用: 人 WB, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 5 (CXCR5) (AA 281-372) antibodyCXCR5 适用: 人, 小鼠 WB, ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 5 (CXCR5) (N-Term) antibodyCXCR5 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 5 (CXCR5) (AA 1-30), (N-Term) antibodyCXCR5 适用: 人 WB 宿主: 兔 Polyclonal RB41684 unconjugated
anti-Chemokine (C-X-C Motif) Receptor 5 (CXCR5) antibodyCXCR5 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 5 (CXCR5) (AA 1-372) antibodyCXCR5 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 5 (CXCR5) (AA 1-55) antibodyCXCR5 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C1 unconjugated
anti-Chemokine (C-X-C Motif) Receptor 5 (CXCR5) (AA 23-38), (Extracellular), (N-Term) antibodyCXCR5 适用: 人 WB, FACS, IF, ICC, LCI 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "A B-cell-homing chemokine made in lymphoid follicles activates Burkitt's lymphoma receptor-1." in: Nature, Vol. 391, Issue 6669, pp. 799-803, (1998) (PubMed).
: "B cell-attracting chemokine 1, a human CXC chemokine expressed in lymphoid tissues, selectively attracts B lymphocytes via BLR1/CXCR5." in: The Journal of experimental medicine, Vol. 187, Issue 4, pp. 655-60, (1998) (PubMed).
: "Expression of the G-protein--coupled receptor BLR1 defines mature, recirculating B cells and a subset of T-helper memory cells." in: Blood, Vol. 84, Issue 3, pp. 830-40, (1994) (PubMed).
: "Cloning of a novel putative G-protein-coupled receptor (NLR) which is expressed in neuronal and lymphatic tissue." in: FEBS letters, Vol. 321, Issue 2-3, pp. 173-8, (1993) (PubMed).
: "Differentiation-specific expression of a novel G protein-coupled receptor from Burkitt's lymphoma." in: European journal of immunology, Vol. 22, Issue 11, pp. 2795-9, (1992) (PubMed).
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: "A B-cell-homing chemokine made in lymphoid follicles activates Burkitt's lymphoma receptor-1." in: Nature, Vol. 391, Issue 6669, pp. 799-803, (1998) (PubMed).
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- 抗原
- CXCR5 (Chemokine (C-X-C Motif) Receptor 5 (CXCR5))
- 别名
- CXCR5 (CXCR5 产品)
- 别名
- BLR1 antibody, CRL1 antibody, CD185 antibody, MDR15 antibody, Blr1 antibody, CXC-R5 antibody, CXCR-5 antibody, Gpcr6 antibody, NLR antibody, C-X-C motif chemokine receptor 5 antibody, chemokine (C-X-C motif) receptor 5 antibody, CXCR5 antibody, Cxcr5 antibody
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