Cutaneous Lymphocyte Antigen 抗体 (Biotin)
Cited in 5+ publications.
The Biotin-conjugated 大鼠 单克隆 anti-Cutaneous Lymphocyte Antigen antibody (Clone HECA) (ABIN2689480) specifically detects Cutaneous Lymphocyte Antigen in FACS, IHC (f) 和 IHC (fro).
The antibody is reactive with 人 和 小鼠 samples.
(5 references)
产品编号 ABIN2689480
发货至:
中国
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Quick Overview for Cutaneous Lymphocyte Antigen 抗体 (Biotin) (ABIN2689480)
抗原
Cutaneous Lymphocyte Antigen
适用
人, 小鼠
宿主
大鼠
克隆类型
单克隆
标记
Biotin
应用范围
Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro))
克隆位点
HECA
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产品特性
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Reacts with cutaneous lymphocyte associated antigen (CLA), a carbohydrate domain shared by sialyl Lewis[x] (sLe[x]) and sialyl Lewis[a] (sLe[a]) antigens. It serves as the ligand for selectins including CD62E (ELAM-1) and CD62L (LECAM-1). CLA is expressed on lymphocytes in the skin on high endothelium. In peripheral blood, it is expressed on a small subset of lympocytes and all monocytes and granulocytes. It has been suggested that CLA plays a role in supporting lymphocyte migration to extravascular tissue during inflammation. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of peripheral blood lymphocytes analyzed by flow cytometry
BD Pharmingen™ Biotin Rat Anti-Human Cutaneous Lymphocyte Antigen - Biotin - Clone HECA-452 - Isotype Rat IgM, κ - Reactivity Hu, Ms - 100 Tests -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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亚型
- IgM kappa
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 20 μL
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限制
- 仅限研究用
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缓冲液
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "The binding of T cell-expressed P-selectin glycoprotein ligand-1 to E- and P-selectin is differentially regulated." in: The Journal of biological chemistry, Vol. 272, Issue 45, pp. 28786-92, (1997) (PubMed).
: "ELAM-1 is an adhesion molecule for skin-homing T cells." in: Nature, Vol. 349, Issue 6312, pp. 796-9, (1991) (PubMed).
: "The neutrophil selectin LECAM-1 presents carbohydrate ligands to the vascular selectins ELAM-1 and GMP-140." in: Cell, Vol. 66, Issue 5, pp. 921-33, (1991) (PubMed).
: "High endothelial differentiation in human lymphoid and inflammatory tissues defined by monoclonal antibody HECA-452." in: The American journal of pathology, Vol. 130, Issue 1, pp. 147-55, (1988) (PubMed).
: "The use of avidin-biotin interaction in immunoenzymatic techniques." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).
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- Cutaneous Lymphocyte Antigen
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背景
- Synonyms: CLA
抗原
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