Crry/p65 抗体
This 大鼠 单克隆 antibody specifically detects Crry/p65 in Func, IHC (fro), FACS, IHC (zinc), IHC (p) 和 ELISA. It exhibits reactivity toward 小鼠. It has been mentioned in 4+ publications
(4 references)
产品编号 ABIN2689475
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Crry/p65 抗体 (ABIN2689475)
抗原
Crry/p65
适用
小鼠
宿主
大鼠
克隆类型
单克隆
应用范围
Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA
克隆位点
1F2
-
-
产品特性
-
The 1F2 mAb reacts with Crry/p65, a cell-surface protein that blocks complement activation and C3 deposition by both the classical and alternative pathways. Based on nucleotide sequence identity, Crry/p65 is the genetic homologue of human CR1 (Complement Receptor 1). However, it has been proposed that Crry/p65 is the functional homologue of human MCP (Membrane Cofactor Protein) and/or DAF (Decay-Accelerating Factor), due to the similar functions these proteins share and the fact that Crry/p65 does not display CR1's complement-receptor activity. Crry/p65 is expressed in most organs, such as thymus, kidney, uterus, skin, lung, pancreas, heart, stomach, large and small intestine, and spleen. Platelets, brain, and muscle express low, but detectable levels of Crry/p65. In addition, Crry/p65 is expressed in J774 cells, bone marrow-derived macrophages, and thioglycollate-elicited peritoneal macrophages, as determined by RT-PCR. Crry is crucial in the regulation of complement activation during early mouse embryonic development and essential in fetomaternal tolerance. The 1F2 mAb partially blocks Crry/p65-mediated complement protection in vitro.
BD Pharmingen™ Purified Rat Anti-Mouse Crry/p65 - Purified - Clone 1F2 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
-
免疫原
- Recombinant soluble Crry/p65 protein
-
亚型
- IgG2a kappa
-
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
限制
- 仅限研究用
-
-
-
浓度
- 0.5 mg/mL
-
缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C
-
储存方法
- Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.
-
-
-
: "A critical role for murine complement regulator crry in fetomaternal tolerance." in: Science (New York, N.Y.), Vol. 287, Issue 5452, pp. 498-501, (2000) (PubMed).
: "Mouse Crry/p65 is a regulator of the alternative pathway of complement activation." in: European journal of immunology, Vol. 23, Issue 6, pp. 1381-4, (1993) (PubMed).
: "Murine macrophages lack expression of the Cr2-145 (CR2) and Cr2-190 (CR1) gene products." in: European journal of immunology, Vol. 23, Issue 11, pp. 3037-42, (1993) (PubMed).
: "The evolution of mouse and human complement C3-binding proteins: divergence of form but conservation of function." in: Immunology today, Vol. 13, Issue 6, pp. 231-6, (1992) (PubMed).
-
-
- Crry/p65
-
别名
- Crry,p65
抗原
-
You are here:
