(5 references)-
- 抗原 See all Chromogranin A (CHGA) 抗体
- Chromogranin A (CHGA)
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This Chromogranin A antibody is un-conjugated
-
应用范围
- Immunofluorescence (IF), Western Blotting (WB), BioImaging (BI), Intracellular Staining (ICS)
- 品牌
- BD Pharmingen™
- 产品特性
-
Chromogranin A (CGA) is a member of the granin family of regulated secretory proteins that are found in secretory granules in endocrine and neuroendocrine cells and released in response to extracellular stimulation. Intracellularly, granins are important for targeting peptide hormones and neurotransmitters by their ability to aggregate in the low pH , high calcium environment of the trans-Golgi network. Extracellularly, peptides formed from proteolytic processing of granins regulate hormone secretion. CGA is a prohormone that can be cleaved into several biologically active peptides, such as pancreastatin, β-granin, vasostatin, catestatin, and parastatin. β-granin is an N-terminal fragment of CGA, while pancreastatin and catestatin are processed from the central region of CGA. Cells of the adrenal medulla, anterior pituitary, cerebral cortex as well as beta cells of the pancreas and a variety of tumor cell lines express CGA. The expression of CGA can be used to monitor the pancreatic differentiation of pluripotent stem cells. Flow cytometric analysis of Chromogranin A expression in human neuroblastoma cells. SH-SY5Y neuroblastoma cells (ATCC CRL-2266) were fixed and permeabilized using BD Cytofix/Cytoperm™ Fixation/Permeablization Kit (Cat. No. 554714), washed twice with BD Perm/Wash™ Buffer (Cat. No. 554723) , then stained with either Purified Mouse Anti-Human Chromogranin A or Purified Mouse IgG1, κ Isotype Control (Cat. No.555746) monoclonal antibodies followed by PE Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 550589) second-step reagent. The fixed cells were resuspended in stain buffer and acquired on a BD LSR™ II
BD Pharmingen™ Purified Mouse Anti-Human Chromogranin A - Purified - Clone S21-537 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human Chromogranin A Recombinant Protein
- 克隆位点
- S21
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product CHGA Primary Antibody
-
anti-Chromogranin A (CHGA) (AA 15-148) antibody
CHGA 适用: 人 WB, ELISA, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Chromogranin A (CHGA) (AA 24-154) antibodyCHGA 适用: 小鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chromogranin A (CHGA) antibodyCHGA 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal CGA-413 unconjugated
anti-Chromogranin A (CHGA) antibodyCHGA 适用: 人 IF, IHC, WB 宿主: 兔 Monoclonal unconjugated
anti-Chromogranin A (CHGA) (AA 278-457) antibodyCHGA 适用: 人 FACS, IHC, WB, ELISA 宿主: 小鼠 Monoclonal 5H10B12 unconjugated
anti-Chromogranin A (CHGA) (AA 87-252) antibodyCHGA 适用: 人 FACS, WB, ELISA, ICC 宿主: 小鼠 Monoclonal 1A6B6 unconjugated
anti-Chromogranin A (CHGA) (AA 17-446) antibodyCHGA 适用: Pig IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chromogranin A (CHGA) (AA 278-457) antibodyCHGA 适用: 人 FACS, IHC, WB, ELISA 宿主: 小鼠 Monoclonal 1B7A4 unconjugated
anti-Chromogranin A (CHGA) antibodyCHGA 适用: 人, 小鼠 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Chromogranin A (CHGA) antibodyCHGA 适用: 人 IHC, StM 宿主: 兔 Monoclonal CHGA-1731R unconjugated Recombinant Antibody
-
- 应用备注
- Flow Cytometry System. The dotted line shows isotype control background staining, while the solid line represents Chromogranin A staining. The overlay histograms were derived from gated events with forward and side light-scatter characteristics of viable cells. Additionally, Purified Mouse Anti-Human Chromogranin A was negative on human embryonic stem (ES) cells (data not shown). Immunofluorescent staining of Chromogranin A in human neuroblastoma cells. SH-SY5Y neuroblastoma cells (ATCC CRL-2266) were fixed with BD Cytofix™ Fixation Buffer , permeabilized with BD Perm/Wash™ Buffer and stained with Purified Mouse Anti-Human Chromogranin A monoclonal antibody (pseudo-colored green) at 1.2 μg/mL. The second-step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies), and counter staining was with DAPI (pseudo-colored blue). No Chromogranin A staining was observed on human embryonic stem cells (hESC) that were imaged similarly (data not shown). Also, no staining was observed when SH-SY5Y and hESC were stained with second-step reagent alone (data not shown). The image was captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ Software. BD Phosflow™ Perm Buffer III or Triton™ X-100 are also suitable for permeabilization. Western Blot analysis of Chromogranin A in human SH-SY5Y neuroblastoma lysate. SH-SY5Y neuroblastoma (ATCC CRL-2266) cell lysate was probed with Purified Mouse Anti-Human Chromogranin A monoclonal antibody at titrations of 4.0 to 0.007 μg/lane. Data shown in lanes 1,2,3 are two fold dilutions, 0.25 μg, 0.125 μg, 0.063 μg. Chromogranin A is identified as a band of ~70 kDa. 564562 Rev. 1 Page 1 of 2
- 限制
- 仅限研究用
-
- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
-
-
: "Chromogranin A and derived peptides in health and disease." in: Journal of molecular neuroscience : MN, Vol. 48, Issue 2, pp. 347-56, (2012) (PubMed).
: "A scalable system for production of functional pancreatic progenitors from human embryonic stem cells. ..." in: PLoS ONE, Vol. 7, Issue 5, pp. e37004, (2012) (PubMed).
: "Pancreatic endoderm derived from human embryonic stem cells generates glucose-responsive insulin-secreting cells in vivo." in: Nature biotechnology, Vol. 26, Issue 4, pp. 443-52, (2008) (PubMed).
: "Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells." in: Nature biotechnology, Vol. 24, Issue 11, pp. 1392-401, (2006) (PubMed).
: "Human chromogranin A gene. Molecular cloning, structural analysis, and neuroendocrine cell-specific expression." in: The Journal of biological chemistry, Vol. 269, Issue 9, pp. 6918-26, (1994) (PubMed).
-
: "Chromogranin A and derived peptides in health and disease." in: Journal of molecular neuroscience : MN, Vol. 48, Issue 2, pp. 347-56, (2012) (PubMed).
-
- 抗原
- Chromogranin A (CHGA)
- 别名
- Chromogranin A (CHGA 产品)
- 别名
- CGA antibody, CG-ALPHA antibody, FSHA antibody, GPHA1 antibody, GPHa antibody, HCG antibody, LHA antibody, TSHA antibody, ChrA antibody, fj05f09 antibody, si:dkey-177p2.2 antibody, wu:fj05f09 antibody, zgc:101749 antibody, zgc:56075 antibody, CHGA antibody, cgA antibody, chga antibody, chromogranin A antibody, glycoprotein hormones, alpha polypeptide antibody, uncharacterized CHGA antibody, chromogranin A S homeolog antibody, CHGA antibody, CGA antibody, Chga antibody, chga antibody, chga.S antibody
- 背景
- Synonyms: CGA, CgA, CHGA, Chromogranin-A, CMGA, SP-I
- 途径
- Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling
-
You are here:
