CD8B 抗体 (Biotin)
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北京 101111
Quick Overview for CD8B 抗体 (Biotin) (ABIN2689440)
抗原
See all CD8B 抗体适用
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克隆类型
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应用范围
克隆位点
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产品特性
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The 341 antibody reacts with the β chain of the CD8 antigen on most thymocytes and a subpopulation of mature T lymphocytes (ie, MHC class I-restricted T cells, including most T suppressor/cytotoxic cells). The CD8 α and β chains (CD8a and CD8b, respectively) form a heterodimer on the surface of most thymocytes and thymus-dependent T suppressor/cytotoxic cells, whereas intestinal intraepithelial lymphocytes, many CD8+ T cells of athymic rats, many activated CD4+ T cells, and most NK cells express CD8a without CD8b. It has been suggested that the expression of the CD8a/CD8b heterodimer is restricted to thymus-derived T lymphocytes. CD8 is an antigen co-receptor on the T cell surface which interacts with MHC class I molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck. Macrophages have also been reported to express CD8 α and β chains, which are involved in signal transduction. The 341 mAb blocks proliferative and cytotoxic in vitro responses of CD8+ effectors to allogeneic cells. Two-color analysis of the expression of CD8b on rat spleen cells. Lewis rat splenocytes were simultaneously stained with FITC-conjugated anti-rat CD8a mAb OX-8 (Cat. no. 554856) and biotinylated mAb 341 (Right panel ), followed by Streptavidin-PE (Cat. no. 554061). Note that the CD8a+CD8b- population represents NK cells. Flow cytometry was performed on a BD FACScan™ flow cytometry system.
BD Pharmingen™ Biotin Mouse Anti-Rat CD8b - Biotin - Clone 341 - Isotype Mouse IgG1, κ - Reactivity Rat - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- CD8-positive Wistar rat splenic T-cell hybridomas
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亚型
- IgG1 kappa
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anti-CD8b Molecule (CD8B) (AA 51-150) antibody
CD8B 适用: 人 WB, ELISA, ICC, IHC (fro), IF (cc), IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD8b Molecule (CD8B) antibodyCD8B 适用: 大鼠 FACS, WB, IP, IHC (fro) 宿主: 小鼠 Monoclonal 341 unconjugated
anti-CD8b Molecule (CD8B) antibody (FITC)CD8B 适用: 大鼠 FACS 宿主: 小鼠 Monoclonal 341 FITC
anti-CD8b Molecule (CD8B) antibody (PE)CD8B 适用: 大鼠 FACS 宿主: 小鼠 Monoclonal 341 PE
anti-CD8b Molecule (CD8B) (AA 22-170) antibodyCD8B 适用: 人 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD8b Molecule (CD8B) (AA 22-170) antibodyCD8B 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-CD8b Molecule (CD8B) (AA 22-170) antibodyCD8B 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD8b Molecule (CD8B) antibody (FITC)CD8B 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal H35-17-2 FITC
anti-CD8b Molecule (CD8B) (AA 1-243) antibodyCD8B 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-CD8b Molecule (CD8B) antibodyCD8B 适用: 大鼠 FACS, WB, IP, Func, IHC (fro) 宿主: 小鼠 Monoclonal 341 unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 4, pp. 1833-40, (1997) (PubMed).
: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, (1992) (PubMed).
: "Differential thymus dependence of rat CD8 isoform expression." in: European journal of immunology, Vol. 22, Issue 11, pp. 2841-8, (1992) (PubMed).
: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, (1989) (PubMed).
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: "A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 4, pp. 1833-40, (1997) (PubMed).
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- CD8B (CD8b Molecule (CD8B))
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别名
- CD8b
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途径
- TCR Signaling
抗原
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