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CD8B 抗体

Name: CD8B 抗体
SKU: ABIN2689439
Availability: OutOfStock

This 小鼠单克隆 antibody specifically detects CD8B in FACS, IP, ELISA, IHC (fro), CyTox 和 IHC (p). It exhibits reactivity toward Pig. It has been mentioned in 10+ publications

(10 references)

产品编号 ABIN2689439

发货至: 中国

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中国
北京 101111

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Quick Overview for CD8B 抗体 (ABIN2689439)

抗原

See all CD8B 抗体

CD8B (CD8b Molecule (CD8B))

适用

Pig

宿主

小鼠

克隆类型

单克隆

标记

This CD8B antibody is un-conjugated

应用范围

Flow Cytometry (FACS), Immunoprecipitation (IP), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytotoxicity Test (CyTox), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

295-33

Discover our top product CD8B Primary Antibody

产品特性

The 295/33-25 antibody reacts with an epitope on the CD8 α chain, a 35 kDa antigen expressed on thymocytes, peripheral T lymphocytes, and NK cells. The CD8 Molecule can exist as a 70 kDa homodimer, composed of α chains, or heterodimer, composed of an α and a β chain. Two peripheral CD8+ T-cell populations can be distinguished in the pig: CD8-bright CD4- CTL effectors/precursors and CD8-dull CD4+ T-helper lymphocytes. Pig NK cells express CD8 (dull staining), CD2, MHC class II, LFA-1, and asialo-GM1, but not CD3, CD4, CD5, or CD6. mAb 295/33-25 was clustered as anti-CD8b at the First International Swine CD workshop. CD8 expression on peripheral blood lymphocytes. Yorkshire pig whole blood was stained with purified 295/33-25 monoclonal antibody followed by FITC-conjugated anti-mouse IgG2a mAb R19-15 (Cat. No. 553390), then PE-conjugated mAb 74-12-4 (anti-pig CD4a, Cat. No. 559586). Erythrocytes were lysed (BD PharmLyse ™, Cat. No. 555899), non-viable leukocytes were excluded by staining with 7-AAD (BD Via-Probe™, Cat. No. 555816/555815), and lymphocytes were gated according to scatter profile. Flow cytometry was performed on a BD FACSCalibur™

BD Pharmingen™ Purified Mouse Anti-Pig CD8b - Purified - Clone 295/33-25 - Isotype Mouse IgG2a, κ - Reactivity Pig - 0.1 mg

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Pig thymocytes

亚型

IgG2a kappa
anti-CD8b Molecule (CD8B) (AA 51-150) antibody
CD8B 适用: 人 WB, ELISA, ICC, IHC (fro), IF (cc), IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-CD8b Molecule (CD8B) antibody
CD8B 适用: 大鼠 FACS, WB, IP, IHC (fro) 宿主: 小鼠 Monoclonal 341 unconjugated

anti-CD8b Molecule (CD8B) antibody (FITC)
CD8B 适用: 大鼠 FACS 宿主: 小鼠 Monoclonal 341 FITC

anti-CD8b Molecule (CD8B) antibody (PE)
CD8B 适用: 大鼠 FACS 宿主: 小鼠 Monoclonal 341 PE

anti-CD8b Molecule (CD8B) (AA 22-170) antibody
CD8B 适用: 人 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-CD8b Molecule (CD8B) (AA 22-170) antibody
CD8B 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-CD8b Molecule (CD8B) (AA 22-170) antibody
CD8B 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-CD8b Molecule (CD8B) antibody (FITC)
CD8B 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal H35-17-2 FITC

anti-CD8b Molecule (CD8B) (AA 1-243) antibody
CD8B 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-CD8b Molecule (CD8B) antibody
CD8B 适用: 大鼠 FACS, WB, IP, Func, IHC (fro) 宿主: 小鼠 Monoclonal 341 unconjugated

应用备注

Flow Cytometry System.

限制

仅限研究用
浓度

0.5 mg/mL

缓冲液

Aqueous buffered solution containing ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store undiluted at 4°C.
Saalmüller, Werner, Fachinger: "T-helper cells from naive to committed." in: Veterinary immunology and immunopathology, Vol. 87, Issue 3-4, pp. 137-45, (2002) (PubMed).

Zuckermann: "Extrathymic CD4/CD8 double positive T cells." in: Veterinary immunology and immunopathology, Vol. 72, Issue 1-2, pp. 55-66, (2000) (PubMed).

Saalmüller, Pauly, Höhlich, Pfaff: "Characterization of porcine T lymphocytes and their immune response against viral antigens." in: Journal of biotechnology, Vol. 73, Issue 2-3, pp. 223-33, (1999) (PubMed).

Zuckermann, Pescovitz, Aasted, Dominguez, Trebichavsky, Novikov, Valpotic, Nielsen, Arn, Sachs, Lunney, Boyd, Walker, Lee, Davis, Barbosa, Saalmüller: "Report on the analyses of mAb reactive with porcine CD8 for the second international swine CD workshop." in: Veterinary immunology and immunopathology, Vol. 60, Issue 3-4, pp. 291-303, (1998) (PubMed).

Saalmüller, Aasted, Canals, Dominguez, Goldman, Lunney, Maurer, Pescovitz, Pospisil, Salmon: "Analyses of mAb reactive with porcine CD8." in: Veterinary immunology and immunopathology, Vol. 43, Issue 1-3, pp. 249-54, (1995) (PubMed).

Saalmüller, Hirt, Maurer, Weiland: "Discrimination between two subsets of porcine CD8+ cytolytic T lymphocytes by the expression of CD5 antigen." in: Immunology, Vol. 81, Issue 4, pp. 578-83, (1994) (PubMed).

Dato, Kim: "Characterization and utilization of a monoclonal antibody inhibiting porcine natural killer cell activity for isolation of natural killer and killer cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 11, pp. 4452-62, (1990) (PubMed).

Pescovitz, Lowman, Sachs: "Expression of T-cell associated antigens by porcine natural killer cells." in: Immunology, Vol. 65, Issue 2, pp. 267-71, (1989) (PubMed).

Jonjić, Jonjić, Saalmüller, Rukavina, Koszinowski: "Distribution of T-lymphocyte subsets in porcine lymphoid tissues." in: Immunology, Vol. 60, Issue 3, pp. 395-401, (1987) (PubMed).

Jonjić, Koszinowski: "Monoclonal antibodies reactive with swine lymphocytes. I. Antibodies to membrane structures that define the cytolytic T lymphocyte subset in the swine." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 133, Issue 2, pp. 647-52, (1984) (PubMed).
抗原

CD8B (CD8b Molecule (CD8B))

别名

CD8b

途径

TCR Signaling