CD8B 抗体
(10 references)Quick Overview for CD8B 抗体 (ABIN2689439)
抗原
See all CD8B 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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品牌
- BD Pharmingen™
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产品特性
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The 295/33-25 antibody reacts with an epitope on the CD8 α chain, a 35 kDa antigen expressed on thymocytes, peripheral T lymphocytes, and NK cells. The CD8 Molecule can exist as a 70 kDa homodimer, composed of α chains, or heterodimer, composed of an α and a β chain. Two peripheral CD8+ T-cell populations can be distinguished in the pig: CD8-bright CD4- CTL effectors/precursors and CD8-dull CD4+ T-helper lymphocytes. Pig NK cells express CD8 (dull staining), CD2, MHC class II, LFA-1, and asialo-GM1, but not CD3, CD4, CD5, or CD6. mAb 295/33-25 was clustered as anti-CD8b at the First International Swine CD workshop. CD8 expression on peripheral blood lymphocytes. Yorkshire pig whole blood was stained with purified 295/33-25 monoclonal antibody followed by FITC-conjugated anti-mouse IgG2a mAb R19-15 (Cat. No. 553390), then PE-conjugated mAb 74-12-4 (anti-pig CD4a, Cat. No. 559586). Erythrocytes were lysed (BD PharmLyse ™, Cat. No. 555899), non-viable leukocytes were excluded by staining with 7-AAD (BD Via-Probe™, Cat. No. 555816/555815), and lymphocytes were gated according to scatter profile. Flow cytometry was performed on a BD FACSCalibur™
BD Pharmingen™ Purified Mouse Anti-Pig CD8b - Purified - Clone 295/33-25 - Isotype Mouse IgG2a, κ - Reactivity Pig - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Pig thymocytes
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亚型
- IgG2a kappa
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anti-CD8b Molecule (CD8B) (AA 51-150) antibody
CD8B 适用: 人 WB, ELISA, IHC (fro), ICC, IF (cc), IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD8b Molecule (CD8B) antibodyCD8B 适用: 大鼠 FACS, WB, IP, IHC (fro) 宿主: 小鼠 Monoclonal 341 unconjugated
anti-CD8b Molecule (CD8B) antibody (FITC)CD8B 适用: 大鼠 FACS 宿主: 小鼠 Monoclonal 341 FITC
anti-CD8b Molecule (CD8B) antibody (PE)CD8B 适用: 大鼠 FACS 宿主: 小鼠 Monoclonal 341 PE
anti-CD8b Molecule (CD8B) (AA 22-170) antibodyCD8B 适用: 人 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD8b Molecule (CD8B) (AA 22-170) antibodyCD8B 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD8b Molecule (CD8B) (AA 1-243) antibodyCD8B 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-CD8b Molecule (CD8B) antibodyCD8B 适用: 大鼠 FACS, WB, IP, IHC (fro), Func 宿主: 小鼠 Monoclonal 341 unconjugated
anti-CD8b Molecule (CD8B) antibody (PE)CD8B 适用: Cow, 绵羊, 山羊, Water Buffalo FACS 宿主: 小鼠 Monoclonal CC58 PE
anti-CD8b Molecule (CD8B) antibody (PE)CD8B 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal H35-17-2 PE
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应用备注
- Flow Cytometry System.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "T-helper cells from naive to committed." in: Veterinary immunology and immunopathology, Vol. 87, Issue 3-4, pp. 137-45, (2002) (PubMed).
: "Extrathymic CD4/CD8 double positive T cells." in: Veterinary immunology and immunopathology, Vol. 72, Issue 1-2, pp. 55-66, (2000) (PubMed).
: "Characterization of porcine T lymphocytes and their immune response against viral antigens." in: Journal of biotechnology, Vol. 73, Issue 2-3, pp. 223-33, (1999) (PubMed).
: "Report on the analyses of mAb reactive with porcine CD8 for the second international swine CD workshop." in: Veterinary immunology and immunopathology, Vol. 60, Issue 3-4, pp. 291-303, (1998) (PubMed).
: "Analyses of mAb reactive with porcine CD8." in: Veterinary immunology and immunopathology, Vol. 43, Issue 1-3, pp. 249-54, (1995) (PubMed).
: "Discrimination between two subsets of porcine CD8+ cytolytic T lymphocytes by the expression of CD5 antigen." in: Immunology, Vol. 81, Issue 4, pp. 578-83, (1994) (PubMed).
: "Characterization and utilization of a monoclonal antibody inhibiting porcine natural killer cell activity for isolation of natural killer and killer cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 11, pp. 4452-62, (1990) (PubMed).
: "Expression of T-cell associated antigens by porcine natural killer cells." in: Immunology, Vol. 65, Issue 2, pp. 267-71, (1989) (PubMed).
: "Distribution of T-lymphocyte subsets in porcine lymphoid tissues." in: Immunology, Vol. 60, Issue 3, pp. 395-401, (1987) (PubMed).
: "Monoclonal antibodies reactive with swine lymphocytes. I. Antibodies to membrane structures that define the cytolytic T lymphocyte subset in the swine." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 133, Issue 2, pp. 647-52, (1984) (PubMed).
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: "T-helper cells from naive to committed." in: Veterinary immunology and immunopathology, Vol. 87, Issue 3-4, pp. 137-45, (2002) (PubMed).
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- CD8B (CD8b Molecule (CD8B))
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别名
- CD8b
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途径
- TCR Signaling
抗原
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