CD8B 抗体
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- 抗原 See all CD8B 抗体
- CD8B (CD8b Molecule (CD8B))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This CD8B antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytotoxicity Test (CyTox), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))
- 品牌
- BD Pharmingen™
- 产品特性
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The H35-17.2 antibody reacts with both alloantigeneic forms of the β chain of the CD8 differentiation antigen (Ly-3 or Lyt- 3). The CD8 α and α' chains (CD8a) form heterodimers with the CD8 β chain (CD8b, Ly-3, or Lyt-3) on the surface of most thymocytes. A subpopulation of mature T lymphocytes (i.e., MHC class I-restricted T cells, including most T suppressor/cytotoxic cells) expresses almost exclusively the CD8 αβ heterodimer (the α' chain is absent). Subsets of γδ TCR-bearing T cells, intestinal intraepithelial lymphocytes, and dendritic cells express CD8a without CD8b. It has been suggested that the expression of the CD8a/CD8b heterodimer is restricted to T lymphocytes which matured in the thymus or in an extrathymic environment that had been influenced by thymus- initiated neuroendocrine signals. CD8 is an antigen coreceptor on the T-cell surface which interacts with MHC class I molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck (p56lck). The H35-17.2 mAb blocks T-cell-mediated cytolysis of allogeneic lymphoma cells.
BD Pharmingen™ Purified Rat Anti-Mouse CD8b - Purified - Clone H35-17.2 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.1 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- 5-day MLR, C57BL/6 anti-BALB/c
- 克隆位点
- H35
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product CD8B Primary Antibody
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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Development of CD8 alpha alpha+ intestinal intraepithelial T cells in beta 2-microglobulin- and/or TAP1-deficient mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 8, pp. 2710-5, (1996) (PubMed).
: "A subclass of dendritic cells kills CD4 T cells via Fas/Fas-ligand-induced apoptosis." in: The Journal of experimental medicine, Vol. 183, Issue 4, pp. 1789-96, (1996) (PubMed).
: "TAP-independent selection of CD8+ intestinal intraepithelial lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 11, pp. 4209-16, (1996) (PubMed).
: "Mouse thymus dendritic cells: kinetics of development and changes in surface markers during maturation." in: European journal of immunology, Vol. 25, Issue 2, pp. 418-25, (1995) (PubMed).
: "The CD8 coreceptor revisited: one chain good, two chains better." in: Immunity, Vol. 1, Issue 4, pp. 243-6, (1995) (PubMed).
: "Requirement for CD8 beta chain in positive selection of CD8-lineage T cells." in: Science (New York, N.Y.), Vol. 263, Issue 5150, pp. 1131-3, (1994) (PubMed).
: "Thymus-neuroendocrine interactions in extrathymic T cell development." in: Science (New York, N.Y.), Vol. 265, Issue 5180, pp. 1860-2, (1994) (PubMed).
: "The roles of CD8 in cytotoxic T lymphocyte function." in: Immunology today, Vol. 14, Issue 4, pp. 183-8, (1993) (PubMed).
: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, (1992) (PubMed).
: "Extrathymic differentiation of intraepithelial lymphocytes: generation of a separate and unequal T-cell repertoire?" in: Immunology today, Vol. 12, Issue 12, pp. 436-8, (1992) (PubMed).
: "Failure of T cell receptor V beta negative selection in murine intestinal intra-epithelial lymphocytes." in: International immunology, Vol. 3, Issue 10, pp. 1005-13, (1992) (PubMed).
: "The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells." in: The Journal of experimental medicine, Vol. 176, Issue 1, pp. 47-58, (1992) (PubMed).
: "Phenotypic complexity of intraepithelial lymphocytes of the small intestine." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 6, pp. 1746-51, (1991) (PubMed).
: "Selective expression of CD8 alpha (Ly-2) subunit on activated thymic gamma/delta cells." in: European journal of immunology, Vol. 20, Issue 4, pp. 927-30, (1990) (PubMed).
: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, (1989) (PubMed).
: "Comparison of thymic and peripheral T cell Ly-2/3 antigens." in: European journal of immunology, Vol. 14, Issue 10, pp. 906-10, (1984) (PubMed).
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Development of CD8 alpha alpha+ intestinal intraepithelial T cells in beta 2-microglobulin- and/or TAP1-deficient mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 8, pp. 2710-5, (1996) (PubMed).
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- 抗原
- CD8B (CD8b Molecule (CD8B))
- 别名
- CD8b (CD8B 产品)
- 别名
- Cd8b antibody, Ly-3 antibody, Ly-C antibody, Lyt-3 antibody, CD8B1 antibody, LEU2 antibody, LY3 antibody, LYT3 antibody, P37 antibody, Cd8b1 antibody, CD8b molecule antibody, CD8 antigen, beta chain 1 antibody, Cd8b antibody, CD8B antibody, Cd8b1 antibody
- 背景
- Synonyms: Ly-3
- 途径
- TCR Signaling
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