CD8 alpha 抗体
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Quick Overview for CD8 alpha 抗体 (ABIN2689434)
抗原
See all CD8 alpha (CD8A) 抗体适用
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克隆类型
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应用范围
克隆位点
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产品特性
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The 53-6.7 antibody monoclonal antibody specifically binds to the 38 kDa α and 34 kDa α' chains of the CD8 differentiation antigen (Ly-2 or Lyt-2) of all mouse strains tested. The CD8 α and α' chains (CD8a) form heterodimers with the CD8 β chain (CD8b, Ly-3, or Lyt-3) on the surface of most thymocytes. A subpopulation of mature T lymphocytes (i.e., MHC class I-restricted T cells, including most T suppressor/cytotoxic cells) expresses almost exclusively the CD8 αβ heterodimer (the α' chain is absent). Subsets of γδ TCR-bearing T cells, intestinal intrapithelial lymphocytes, and dendritic cells express CD8a without CD8b. It has been suggested that the expression of the CD8a/CD8b heterodimer is restricted to T lymphocytes which matured in the thymus or in an extrathymic environment that had been influenced by thymus-initiated neuroendocrine signals. CD8 is an antigen coreceptor on the T-cell surface which interacts with MHC class I molecules on antigen-presenting cells or epithelial cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck (p56 [lck]). The CD8 α and α' chains arise from alternatively spliced messengers of a single CD8a gene. The longer α form associates with p56 [lck] via a CXCP motif in its cytoplasmic domain, which it shares with CD4, but not with CD8b. The truncated α' chain is unable to associate with p56 [lck], and it may function to attenuate the CD8-mediated costimulatory signal during intrathymic T-cell maturation. In vivo and in vitro treatment with 53-6.7 mAb has reportedly been effective at depleting CD8+ peripheral T lymphocytes. The 53-6.7 antibody has also been reported to cross-reaact with CD8 α- and α'-like polypeptides on subsets of thymic and peripheral lymphocytes in the Egyptian toad, Bufo regularis. Immunohistochemical staining of CD8+ T lymphocytes. Frozen sections of normal mouse spleen was reacted with 53-6.7 antibody. CD8+ T lymphocytes can be identified by the intense brown labeling of their cell surface membranes.
BD Pharmingen™ Purified Rat Anti-Mouse CD8a - Purified - Clone 53-6.7 - Isotype Rat IgG2a, κ - Reactivity Ms - 1 mL -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Mouse thymus / spleen Cells
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亚型
- IgG2a kappa
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anti-CD8a Molecule (CD8A) (AA 22-235) antibody
CD8A 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 8H8A8 unconjugated
anti-CD8a Molecule (CD8A) (AA 51-150) antibodyCD8A 适用: 人, 大鼠, 小鸡 FACS, WB, ELISA, IHC (fro), IHC (p), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-CD8a Molecule (CD8A) antibodyCD8A 适用: 人 IHC, WB, IP, ELISA 宿主: 兔 Monoclonal 2F2 unconjugated Recombinant Antibody
anti-CD8a Molecule (CD8A) antibodyCD8A 适用: 人 FACS, WB, IF (p) 宿主: 兔 Monoclonal 23GB1420 unconjugated Recombinant Antibody
anti-CD8a Molecule (CD8A) antibodyCD8A 适用: 小鼠 FACS, IP, ICC, IHC (fro), IHC (p) 宿主: 大鼠 Monoclonal 53-6-7 unconjugated
anti-CD8a Molecule (CD8A) antibodyCD8A 适用: 小鼠 FACS, IP, ICC, IHC (fro), IHC (p), Func 宿主: 大鼠 Monoclonal 53-6-7 unconjugated
anti-CD8a Molecule (CD8A) antibodyCD8A 适用: 人 IHC, StM, ISt 宿主: 小鼠 Monoclonal C8-468 unconjugated
anti-CD8a Molecule (CD8A) (AA 22-182) antibodyCD8A 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 6A9A5 unconjugated
anti-CD8a Molecule (CD8A) (AA 28-196) antibodyCD8A 适用: 小鼠, 大鼠 FACS, IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD8a Molecule (CD8A) (AA 150-180) antibodyCD8A 适用: 人 FACS, IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 62.5 μg/mL
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缓冲液
- Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Role of antigen, CD8, and cytotoxic T lymphocyte (CTL) avidity in high dose antigen induction of apoptosis of effector CTL." in: The Journal of experimental medicine, Vol. 184, Issue 2, pp. 485-92, (1996) (PubMed).
: "TAP-independent selection of CD8+ intestinal intraepithelial lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 11, pp. 4209-16, (1996) (PubMed).
: "Thymus-neuroendocrine interactions in extrathymic T cell development." in: Science (New York, N.Y.), Vol. 265, Issue 5180, pp. 1860-2, (1994) (PubMed).
: "Requirement for CD8 beta chain in positive selection of CD8-lineage T cells." in: Science (New York, N.Y.), Vol. 263, Issue 5150, pp. 1131-3, (1994) (PubMed).
: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, (1992) (PubMed).
: "CD4 and CD8 regulate interleukin 2 responses of T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 12, pp. 5557-61, (1992) (PubMed).
: "Inability of CD8 alpha' polypeptides to associate with p56lck correlates with impaired function in vitro and lack of expression in vivo." in: Nature, Vol. 342, Issue 6247, pp. 278-81, (1989) (PubMed).
: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, (1989) (PubMed).
: "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." in: Immunological reviews, Vol. 47, pp. 63-90, (1980) (PubMed).
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: "Role of antigen, CD8, and cytotoxic T lymphocyte (CTL) avidity in high dose antigen induction of apoptosis of effector CTL." in: The Journal of experimental medicine, Vol. 184, Issue 2, pp. 485-92, (1996) (PubMed).
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- CD8 alpha (CD8A) (CD8a Molecule (CD8A))
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别名
- CD8a
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背景
- Synonyms: Cd8a, CD8 alpha chain, Ly-2, Lyt2, Lyt-2, Ly-35, Ly-B
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途径
- TCR Signaling
抗原
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