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CD86 抗体

Name: CD86 抗体
SKU: ABIN2689420
Availability: OutOfStock

Cited in 15+ publications. The 大鼠单克隆 anti-CD86 antibody (Clone GL1) (ABIN2689420) specifically detects CD86 in FACS, IP, IF, IHC (fro) 和 BR. The antibody is reactive with 小鼠 samples.

(15 references)

产品编号 ABIN2689420

发货至: 中国

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Quick Overview for CD86 抗体 (ABIN2689420)

抗原

See all CD86 抗体

CD86

适用

小鼠

宿主

大鼠

克隆类型

单克隆

标记

This CD86 antibody is un-conjugated

应用范围

Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR)

克隆位点

GL1

Discover our top product CD86 Primary Antibody

产品特性

The GL1 antibody has been reported to react with the B7-2 (CD86) costimulatory molecule expressed on a broad spectrum of leukocytes, including B lymphocytes, T lymphocytes, thioglycollate-induced peritoneal macrophages, dendritic cells and astrocytes. CD86 is expressed at low levels by freshly explanted peripheral B and T cells, and its expression is substantially increased by a variety of T cell- and B cell-specific stimuli with a peak expression after 18-42 hours of culture. In contrast to most naive CD4+ T cells, memory CD4+ T cells express B7-2, both at the mRNA and protein level. CD86, a ligand for CD28 and CD152 (CTLA-4), is one of the accessory molecules that plays an important role in T cell-B cell costimulatory interactions. It has been shown to be involved in immunoglobulin class-switching and triggering of mouse NK cell-mediated cytotoxicity. CD80 (B7-1) is an alternate ligand for CD28 and CD152 (CTLA-4). GL1 antibody reportedly blocks MLR and stimulation of T cells by natural antigen-presenting cells. In addition, a mixture of anti-B7-1 and anti B7-2 (GL1) mAbs reportedly inhibits the in vitro interaction of CTLA-4 with its ligand and the in vivo priming of cytotoxic T lymphocytes. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Upregulation of membrane CD86 (B7-2) on activated B lymphocytes. Freshly isolated (left panel) or 72-hour LPS-stimulated BALB/c splenocytes (right panel) were pretreated with Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. No. 553141/553142) and either unstained (shaded histograms) or stained with purified GL1 mAb (open histograms) followed by FITC-conjugated anti-rat IgG2a mAb RG7/1.30 (Cat. No. 553896, shaded and open histograms). Flow cytometry was performed on a BD FACScan™

BD Pharmingen™ Purified Rat Anti-Mouse CD86 - Purified - Clone GL1 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Mouse (CBA/Ca) LPS-activated splenic B Cells

亚型

IgG2a kappa
anti-CD86 (CD86) (AA 140-175) antibody
CD86 适用: 人, 小鼠, 大鼠, 犬 FACS, WB, ELISA, IHC (fro), ICC, IHC (p), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-CD86 (CD86) (AA 37-272) antibody
CD86 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-CD86 (CD86) (AA 30-247) antibody
CD86 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-CD86 (CD86) antibody
CD86 适用: 人, 小鼠, 大鼠 FACS, WB, IHC, IF, ELISA, BP, StM, Coat 宿主: 兔 Monoclonal C86-2160R unconjugated Recombinant Antibody

anti-CD86 (CD86) antibody
CD86 适用: 人, 小鼠, 大鼠 FACS, IHC, IF, Func, BP, StM 宿主: 小鼠 Monoclonal BU63 unconjugated

anti-CD86 (CD86) antibody
CD86 适用: 人 FACS, WB, IP, IHC (fro) 宿主: 小鼠 Monoclonal BU63 unconjugated

anti-CD86 (CD86) antibody (FITC)
CD86 适用: 人 FACS 宿主: 小鼠 Monoclonal BU63 FITC

anti-CD86 (CD86) antibody
CD86 适用: 人, 小鼠, 大鼠 WB, IHC, IF, BP, StM 宿主: 小鼠 Monoclonal C86-1146 unconjugated

anti-CD86 (CD86) antibody
CD86 适用: 人, 小鼠, 大鼠 FACS, IHC, IF, BP, StM 宿主: 小鼠 Monoclonal rC86-1146 unconjugated Recombinant Antibody

anti-CD86 (CD86) antibody
CD86 适用: 人 FACS, WB, IP, IHC (fro), Func 宿主: 小鼠 Monoclonal BU63 unconjugated

应用备注

Flow Cytometry System. Resting lymphocytes (left panel) or activated blasts (right panel) were selected according to light-scatter profile.

限制

仅限研究用
浓度

0.5 mg/mL

缓冲液

Aqueous buffered solution containing ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store undiluted at 4°C.
Turley, Inaba, Garrett, Ebersold, Unternaehrer, Steinman, Mellman: "Transport of peptide-MHC class II complexes in developing dendritic cells." in: Science (New York, N.Y.), Vol. 288, Issue 5465, pp. 522-7, (2000) (PubMed).

Martín-Fontecha, Assarsson, Carbone, Kärre, Ljunggren: "Triggering of murine NK cells by CD40 and CD86 (B7-2)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 10, pp. 5910-6, (1999) (PubMed).

McAdam, Schweitzer, Sharpe: "The role of B7 co-stimulation in activation and differentiation of CD4+ and CD8+ T cells." in: Immunological reviews, Vol. 165, pp. 231-47, (1999) (PubMed).

Hakamada-Taguchi, Kato, Ushijima, Murakami, Uede, Nariuchi: "Expression and co-stimulatory function of B7-2 on murine CD4+ T cells." in: European journal of immunology, Vol. 28, Issue 3, pp. 865-73, (1998) (PubMed).

Borriello, Sethna, Boyd, Schweitzer, Tivol, Jacoby, Strom, Simpson, Freeman, Sharpe: "B7-1 and B7-2 have overlapping, critical roles in immunoglobulin class switching and germinal center formation." in: Immunity, Vol. 6, Issue 3, pp. 303-13, (1997) (PubMed).

Herold, Vezys, Koons, Lenschow, Thompson, Bluestone: "CD28/B7 costimulation regulates autoimmune diabetes induced with multiple low doses of streptozotocin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 2, pp. 984-91, (1997) (PubMed).

Liu, Wenger, Zhao, Nielsen: "Distinct costimulatory molecules are required for the induction of effector and memory cytotoxic T lymphocytes." in: The Journal of experimental medicine, Vol. 185, Issue 2, pp. 251-62, (1997) (PubMed).

Nikcevich, Gordon, Tan, Hurst, Kroepfl, Gardinier, Barrett, Miller: "IFN-gamma-activated primary murine astrocytes express B7 costimulatory molecules and prime naive antigen-specific T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 2, pp. 614-21, (1997) (PubMed).

Bluestone: "New perspectives of CD28-B7-mediated T cell costimulation." in: Immunity, Vol. 2, Issue 6, pp. 555-9, (1995) (PubMed).

Hathcock, Laszlo, Pucillo, Linsley, Hodes: "Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function." in: The Journal of experimental medicine, Vol. 180, Issue 2, pp. 631-40, (1994) (PubMed).

Inaba, Witmer-Pack, Inaba, Hathcock, Sakuta, Azuma, Yagita, Okumura, Linsley, Ikehara, Muramatsu, Hodes, Steinman: "The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro." in: The Journal of experimental medicine, Vol. 180, Issue 5, pp. 1849-60, (1994) (PubMed).

Larsen, Ritchie, Hendrix, Linsley, Hathcock, Hodes, Lowry, Pearson: "Regulation of immunostimulatory function and costimulatory molecule (B7-1 and B7-2) expression on murine dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 11, pp. 5208-19, (1994) (PubMed).

Lenschow, Su, Zuckerman, Nabavi, Jellis, Gray, Miller, Bluestone: "Expression and functional significance of an additional ligand for CTLA-4." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 23, pp. 11054-8, (1994) (PubMed).

Freeman, Borriello, Hodes, Reiser, Hathcock, Laszlo, McKnight, Kim, Du, Lombard: "Uncovering of functional alternative CTLA-4 counter-receptor in B7-deficient mice." in: Science (New York, N.Y.), Vol. 262, Issue 5135, pp. 907-9, (1993) (PubMed).

Hathcock, Laszlo, Dickler, Bradshaw, Linsley, Hodes: "Identification of an alternative CTLA-4 ligand costimulatory for T cell activation." in: Science (New York, N.Y.), Vol. 262, Issue 5135, pp. 905-7, (1993) (PubMed).
抗原

CD86

别名

CD86

背景

Synonyms: B7-2

途径

TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation