CD77 抗体
(3 references)Quick Overview for CD77 抗体 (ABIN2689395)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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品牌
- BD Pharmingen™
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产品特性
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Reacts with CD77, a small neutral glycosphingolipid, 1 kDa, also referred to as globotriaosylceramide (Gb3). CD77 is expressed on a subset of germinal center B-lymphocytes and not on peripheral blood lymphocytes, bone marrow or spleen. It has been described as a receptor for Shiga Toxin, produced by Shigella dysenteriae, and Verotoxin 1 (VT1), produced by some strains of E. coli. CD19 has been reported as a ligand for CD77 and these interaction functions in adhesion and signal transduction at a specific stage of B cell development. Studies have shown that cross-linking CD77 induces apoptosis in some B cell lines. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of Ramos cell line analyzed by flow cytometry. Second step staining with Cat. No. 555988.
BD Pharmingen™ Purified Mouse Anti-Human CD77 - Purified - Clone 5B5 - Isotype Mouse IgM, κ - Reactivity Hu - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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亚型
- IgM kappa
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anti-CD77 antibody
适用: 人 FACS, ICC, IHC (fro) 宿主: 大鼠 Monoclonal 38-13 unconjugated
anti-CD77 antibody (FITC)适用: 人 FACS 宿主: 大鼠 Monoclonal 38-13 FITC
anti-CD77 antibody (PE)适用: 人 FACS 宿主: 大鼠 Monoclonal 38-13 PE
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Regulation of germinal center B cell differentiation. Role of the human APO-1/Fas (CD95) molecule." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 11, pp. 5746-56, (1995) (PubMed).
: "The fate of human CD77+ germinal center B lymphocytes after rescue from apoptosis." in: Molecular immunology, Vol. 32, Issue 5, pp. 333-9, (1995) (PubMed).
: "Subpopulations of B lymphocytes in germinal centers." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 139, Issue 7, pp. 2187-94, (1987) (PubMed).
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: "Regulation of germinal center B cell differentiation. Role of the human APO-1/Fas (CD95) molecule." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 11, pp. 5746-56, (1995) (PubMed).
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- CD77
抗原
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