CD69 抗体 (Biotin)
(15 references)Quick Overview for CD69 抗体 (Biotin) (ABIN2689380)
抗原
See all CD69 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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品牌
- BD Pharmingen™
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产品特性
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The H1.2F3 antibody reacts with CD69 (Very Early Activation antigen), an 85 kDa disulfide-linked homodimer of differentially glycosylated subunits. CD69 is a C-type lectin, most closely related to the NKR-P1 and Ly-49 NK cell-activation molecules. Its expression is rapidly induced upon activation of lymphocytes (T, B, NK, and NK-T cells), neutrophils, and macrophages. CD69 is expressed also on thymocytes that are undergoing positive selection, its role in that process is unclear. H1.2F3 mAb augments PMA-induced T-cell stimulation and IFN-γ-induced macrophage stimulation. IL-2-activated NK cells express CD69, and H1.2F3 mAb induces redirected lysis of FcR-bearing target cells by NK cells. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.
BD Pharmingen™ Biotin Hamster Anti-Mouse CD69 - Biotin - Clone H1.2F3 - Isotype Armenian Hamster IgG1, λ3 - Reactivity Ms - 0.5 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Mouse Dendritic Epidermal T Cell Line Y245
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亚型
- IgG1 lambda
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anti-CD69 (CD69) (AA 101-199) antibody
CD69 适用: 人, 小鼠 FACS, WB, ELISA, IHC (fro), IF (p), IF (cc), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD69 (CD69) antibody (FITC)CD69 适用: 人 FACS 宿主: 小鼠 Monoclonal FN50 FITC
anti-CD69 (CD69) antibodyCD69 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 8B6 unconjugated
anti-CD69 (CD69) (AA 1-199) antibodyCD69 适用: 人 FACS, WB 宿主: 小鼠 Polyclonal unconjugated
anti-CD69 (CD69) antibodyCD69 适用: 人 FACS, IHC (fro) 宿主: 小鼠 Monoclonal FN50 unconjugated
anti-CD69 (CD69) antibody (APC)CD69 适用: 人 FACS 宿主: 小鼠 Monoclonal FN50 APC
anti-CD69 (CD69) antibody (PE)CD69 适用: 人 FACS 宿主: 小鼠 Monoclonal FN50 PE
anti-CD69 (CD69) antibody (PE)CD69 适用: 人, Baboon, Cynomolgus, Macaque, 猕猴, Chimpanzee FACS 宿主: 小鼠 Monoclonal FN50 PE
anti-CD69 (CD69) (AA 90-199) antibodyCD69 适用: 人 ELISA, IP 宿主: 小鼠 Monoclonal 4H3 unconjugated
anti-CD69 (CD69) (Internal Region) antibodyCD69 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "Phenotypic and functional characteristics of hematopoietic cell lineages in CD69-deficient mice." in: Blood, Vol. 95, Issue 7, pp. 2312-20, (2000) (PubMed).
: "Regulation of lineage commitment distinct from positive selection." in: Science (New York, N.Y.), Vol. 286, Issue 5442, pp. 1149-53, (1999) (PubMed).
: "Recent thymic emigrants are distinct from most medullary thymocytes." in: European journal of immunology, Vol. 27, Issue 8, pp. 2010-5, (1997) (PubMed).
: "Expression and function of the early activation antigen CD69 in murine macrophages." in: Journal of leukocyte biology, Vol. 62, Issue 3, pp. 349-55, (1997) (PubMed).
: "How many thymocytes audition for selection?" in: The Journal of experimental medicine, Vol. 186, Issue 7, pp. 1149-58, (1997) (PubMed).
: "Lineage commitment in the thymus: only the most differentiated (TCRhibcl-2hi) subset of CD4+CD8+ thymocytes has selectively terminated CD4 or CD8 synthesis." in: The Journal of experimental medicine, Vol. 184, Issue 6, pp. 2091-9, (1997) (PubMed).
: "Positive selection of thymocytes involves sustained interactions with the thymic microenvironment." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 11, pp. 5234-40, (1995) (PubMed).
: "The activation antigen CD69." in: Stem cells (Dayton, Ohio), Vol. 12, Issue 5, pp. 456-65, (1995) (PubMed).
: "Regulation of RAG-1 and CD69 expression in the thymus during positive and negative selection." in: European journal of immunology, Vol. 24, Issue 1, pp. 145-51, (1994) (PubMed).
: "The mouse CD69 gene. Structure, expression, and mapping to the NK gene complex." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 3, pp. 1228-36, (1994) (PubMed).
: "Aberrant expression of the very early activation antigen on MRL/Mp-lpr/lpr lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 2, pp. 673-82, (1993) (PubMed).
: "Activation events during thymic selection." in: The Journal of experimental medicine, Vol. 175, Issue 3, pp. 731-42, (1992) (PubMed).
: "Stimulation of murine natural killer (NK) cells by a monoclonal antibody specific for the NK1.1 antigen. IL-2-activated NK cells possess additional specific stimulation pathways." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 10, pp. 3662-73, (1991) (PubMed).
: "Very early (VEA) and very late (VLA) activation antigens have distinct functions in T lymphocyte activation." in: Immunological reviews, Vol. 109, pp. 153-76, (1989) (PubMed).
: "Characterization of a cell surface-expressed disulfide-linked dimer involved in murine T cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 2, pp. 369-76, (1988) (PubMed).
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: "Phenotypic and functional characteristics of hematopoietic cell lineages in CD69-deficient mice." in: Blood, Vol. 95, Issue 7, pp. 2312-20, (2000) (PubMed).
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- CD69
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别名
- CD69
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背景
- Synonyms: Very Early Activation antigen
抗原
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