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Selectin E/CD62e 抗体 (Biotin)

SELE 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 10E9-6 Biotin
产品编号 ABIN2689358
发货至: 中国
  • 抗原 See all Selectin E/CD62e (SELE) 抗体
    Selectin E/CD62e (SELE) (Selectin E (SELE))
    适用
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    小鼠
    宿主
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    大鼠
    克隆类型
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    单克隆
    标记
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    This Selectin E/CD62e antibody is conjugated to Biotin
    应用范围
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    Flow Cytometry (FACS)
    品牌
    BD Pharmingen™
    产品特性
    The 10E9.6 antibody reacts with the 97-110 kDa cell surface glycoprotein E-selectin (CD62E), also known as endothelial-leukocyte adhesion molecule-1 (ELAM-1), which is expressed on endotoxin- or cytokine-stimulated mouse endothelial cells. A suspension of TNFα stimulated mouse brain capillary endothelioma cells, from the cell line bEnd.3, was used as the immunogen. The epitope recognized by mAb 10E9.6 has been mapped to the first and/or second complement regulatory protein repeat domains of E-selectin. The 10E9.6 antibody has been reported to block binding of a monocyte cell line to E-selectin in vitro and to block neutrophil migration in BALB/c, but not C57BL/6 mice. It has no effect on leukocyte rolling in TNFα-treated mouse venules or on in vitro adhesion of myeloid cells to E-selectin. Studies have demonstrated that Cutaneous Lymphocyte Antigen (CLA), recognized by mAb HECA-452 (Cat. no. 555946), may be a ligand for CD62E. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

    BD Pharmingen™ Biotin Rat Anti-Mouse CD62E - Biotin - Clone 10E9.6 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg
    纯化方法
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    免疫原
    Mouse brain capillary endothelioma bEnd.3 (TNFalpha-stimulated) Cell Line
    克隆位点
    10E9-6
    亚型
    IgG2a kappa
    Top Product
    Discover our top product SELE Primary Antibody
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    限制
    仅限研究用
  • 浓度
    0.5 mg/mL
    缓冲液
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
    储存条件
    4 °C
    储存方法
    Store undiluted at 4°C.
  • Pendl, Robert, Steinert, Thanos, Eytner, Borges, Wild, Lowe, Fuhlbrigge, Kupper, Vestweber, Grabbe: "Immature mouse dendritic cells enter inflamed tissue, a process that requires E- and P-selectin, but not P-selectin glycoprotein ligand 1." in: Blood, Vol. 99, Issue 3, pp. 946-56, (2002) (PubMed).

    Borges, Pendl, Eytner, Steegmaier, Zöllner, Vestweber: "The binding of T cell-expressed P-selectin glycoprotein ligand-1 to E- and P-selectin is differentially regulated." in: The Journal of biological chemistry, Vol. 272, Issue 45, pp. 28786-92, (1997) (PubMed).

    Ramos, Kunkel, Lawrence, Jung, Vestweber, Bosse, McIntyre, Gillooly, Norton, Wolitzky, Ley: "Differential effect of E-selectin antibodies on neutrophil rolling and recruitment to inflammatory sites." in: Blood, Vol. 89, Issue 8, pp. 3009-18, (1997) (PubMed).

    Eppihimer, Wolitzky, Anderson, Labow, Granger: "Heterogeneity of expression of E- and P-selectins in vivo." in: Circulation research, Vol. 79, Issue 3, pp. 560-9, (1996) (PubMed).

    Bosse, Vestweber: "Only simultaneous blocking of the L- and P-selectin completely inhibits neutrophil migration into mouse peritoneum." in: European journal of immunology, Vol. 24, Issue 12, pp. 3019-24, (1995) (PubMed).

    Ley, Bullard, Arbonés, Bosse, Vestweber, Tedder, Beaudet: "Sequential contribution of L- and P-selectin to leukocyte rolling in vivo." in: The Journal of experimental medicine, Vol. 181, Issue 2, pp. 669-75, (1995) (PubMed).

    Weller, Isenmann, Vestweber: "Cloning of the mouse endothelial selectins. Expression of both E- and P-selectin is inducible by tumor necrosis factor alpha." in: The Journal of biological chemistry, Vol. 267, Issue 21, pp. 15176-83, (1992) (PubMed).

  • 抗原
    Selectin E/CD62e (SELE) (Selectin E (SELE))
    别名
    CD62E (SELE 产品)
    别名
    SELE antibody, CD62E antibody, ELAM antibody, ELAM1 antibody, ESEL antibody, LECAM2 antibody, E-selectin antibody, Elam antibody, selectin E antibody, selectin, endothelial cell antibody, SELE antibody, Sele antibody
    背景
    Synonyms: E-selectin, ELAM-1
    途径
    Thromboxane A2 Receptor Signaling
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