ITGA2 抗体
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北京 101111
Quick Overview for ITGA2 抗体 (ABIN2689302)
抗原
See all ITGA2 抗体适用
宿主
克隆类型
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应用范围
克隆位点
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产品特性
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The HMα2 antibody reacts with integrin α2 chain (CD49b), the 150- kDa transmembrane glycoprotein that non-covalently associates with the integrin β1 subunit (CD29) to form the integrin α2β1 complex known as VLA-2. VLA-2, a receptor for collagen and laminin, is expressed on some splenic CD4+ T lymphocytes and NK-T cells, intestinal intraepithelial and lamina propria lymphocytes, splenic NK cells, epithelial cells, and platelets, but it is not on thymocytes or Peyer's-patch or lymphnode lymphocytes. The expression of VLA-2 is upregulated on lymphocytes in response to mitogens. The HMα2 antibody has been reported to partially block the interaction of T-cell blasts, but not NK cells, with collagen. Purified HMα2 mAb blocks the staining of splenic NK cells by the anti-CD49b/Pan-NK Cells mAb DX5 (Cat. No. 553858, for the PE conjugate). Therefore, mAb HMα2 may be used like the DX5 mAb for identification of NK cells. Two-color analysis of CD49b expression on spleen leukocytes. C57BL/6 splenocytes were simultaneously stained with FITC-conjugated mAb M1/70 (anti-mouse CD11b, Cat. No. 557396/553310, both panels) and either purified hamster IgG1, κ isotype control mAb A19-3 (Cat. No. 553969, left panel) or purified HMα2 mAb (right panel), followed by PE-conjugated anti-hamster IgG mAb cocktail (Cat. No. 554056, both panels). Flow cytometry was performed on a BD FACScan™ Flow Cytometry System.
BD Pharmingen™ Purified Hamster Anti-Mouse CD49b - Purified - Clone HMα2 - Isotype Armenian Hamster IgG1, κ - Reactivity Ms - 0.5 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Mouse colon carcinoma cell line Colon26
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亚型
- IgG1 kappa
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anti-Integrin alpha 2 (ITGA2) (AA 30-119) antibody
ITGA2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2B6 unconjugated
anti-Integrin alpha 2 (ITGA2) (AA 994-1132) antibodyITGA2 适用: 人 FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 5E3A8 unconjugated
anti-Integrin alpha 2 (ITGA2) antibodyITGA2 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated
anti-Integrin alpha 2 (ITGA2) (AA 170-361) antibodyITGA2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 2 (ITGA2) (Internal Region) antibodyITGA2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 2 (ITGA2) (C-Term) antibodyITGA2 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 2 (ITGA2) (AA 74-277) antibodyITGA2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 2 (ITGA2) (AA 678-894) antibodyITGA2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 2 (ITGA2) (AA 188-365) antibodyITGA2 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 2 (ITGA2) antibodyITGA2 适用: 人, Baboon, Cynomolgus, 猕猴 FACS, IHC (fro), Func 宿主: 小鼠 Monoclonal AK7 unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Cutting edge: the mouse NK cell-associated antigen recognized by DX5 monoclonal antibody is CD49b (alpha 2 integrin, very late antigen-2)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 3, pp. 1141-4, (2001) (PubMed).
: "A population of CD62Llow Nk1.1- CD4+ T cells that resembles NK1.1+ CD4+ T cells." in: European journal of immunology, Vol. 28, Issue 10, pp. 3172-82, (1998) (PubMed).
: "Involvement of alpha 1 and alpha 4 integrins in gut mucosal injury of graft-versus-host disease." in: International immunology, Vol. 7, Issue 8, pp. 1183-9, (1996) (PubMed).
: "Identification and functional characterization of mouse CD29 with a mAb." in: International immunology, Vol. 7, Issue 5, pp. 835-42, (1995) (PubMed).
: "Identification of collagen and laminin receptor integrins on murine T lymphocytes." in: European journal of immunology, Vol. 24, Issue 9, pp. 2000-5, (1994) (PubMed).
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: "Cutting edge: the mouse NK cell-associated antigen recognized by DX5 monoclonal antibody is CD49b (alpha 2 integrin, very late antigen-2)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 3, pp. 1141-4, (2001) (PubMed).
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- ITGA2 (Integrin alpha 2 (ITGA2))
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别名
- CD49b
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背景
- Synonyms: Integrin α2 chain
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途径
- CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Integrin Complex
抗原
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