CD45RA 抗体 (Biotin)
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Quick Overview for CD45RA 抗体 (Biotin) (ABIN2689287)
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See all CD45RA 抗体适用
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产品特性
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The 14.8 clone has been reported to react with an exon A-dependent epitope of the CD45 protein, which is found at high density on B cells and at low density on peripheral T cytotoxic/suppressor cells and a very small subset of thymocytes. Nearly all B-lineage cells, including B-cell precursors in fetal liver and adult bone marrow and Ig-secreting cells, but not hematopoietic stem cells or myeloid progenitors, have been reported to be detectable by mAb 14.8. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus, differing levels of glycosylation. The CD45 isoforms detected in the mouse are cell type-, maturation-, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction. mAb 14.8 has been reported to enhance the proliferative effect of PHA on purified spleen T cells, possibly by replacing a signal normally delivered by accessory cells, to enhance isotype switching during in vitro B-cell responses, and to inhibit antigen-induced p21 [ras] activation. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.
BD Pharmingen™ Biotin Rat Anti-Mouse CD45RA - Biotin - Clone 14.8 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Radiation-induced NZC mouse B lymphoma WEHI-279
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亚型
- IgG2b kappa
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anti-CD45RA (CD45RA) antibody
CD45RA 适用: 人 FACS, IHC (p), WB, IP, MS 宿主: 小鼠 Monoclonal MEM-56 unconjugated
anti-CD45RA (CD45RA) antibody (PE)CD45RA 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-56 PE
anti-CD45RA (CD45RA) antibodyCD45RA 适用: 人 IHC 宿主: 小鼠 Monoclonal 472M unconjugated
anti-CD45RA (CD45RA) antibody (FITC)CD45RA 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-56 FITC
anti-CD45RA (CD45RA) antibody (APC)CD45RA 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-56 APC
anti-CD45RA (CD45RA) antibodyGMP Grade CD45RA 适用: 人 FACS 宿主: 小鼠 Monoclonal OTH-74D4 unconjugated
anti-CD45RA (CD45RA) antibody (FITC)CD45RA 适用: 人 FACS, IHC, IHC (p), IP, IHC (fro) 宿主: 小鼠 Monoclonal FITC
anti-CD45RA (CD45RA) antibodyCD45RA 适用: 人 FACS, IHC 宿主: 小鼠 Monoclonal B-C15 unconjugated
anti-CD45RA (CD45RA) antibody (Biotin)CD45RA 适用: 人 FACS, IHC (p), WB, IP 宿主: 小鼠 Monoclonal MEM-56 Biotin
anti-CD45RA (CD45RA) antibody (PerCP)CD45RA 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-56 PerCP
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "Ligation of CD45 on B cells can facilitate production of secondary Ig isotypes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 3, pp. 1014-21, (1994) (PubMed).
: "Characterization of constitutive and strain-dependent subsets of CD45RA+ cells in the thymus." in: International immunology, Vol. 4, Issue 11, pp. 1303-11, (1993) (PubMed).
: "Distinction of mouse CD8+ suppressor effector T cell clones from cytotoxic T cell clones by cytokine production and CD45 isoforms." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 6, pp. 2121-8, (1993) (PubMed).
: "Expression of variable exon A-, B-, and C-specific CD45 determinants on peripheral and thymic T cell populations." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 1, pp. 19-28, (1992) (PubMed).
: "CD45 alternative exon expression in murine and human CD4+ T cell subsets." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 12, pp. 4054-65, (1992) (PubMed).
: "Anti-CD45RA antibodies increase the proliferation of mouse T cells to phytohemagglutinin through the interleukin 2/interleukin 2 receptor pathway." in: European journal of immunology, Vol. 19, Issue 11, pp. 2005-10, (1990) (PubMed).
: "Antigens displayed on murine B lymphocyte precursors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 127, Issue 6, pp. 2262-8, (1982) (PubMed).
: "Formation of B lymphocytes in fetal and adult life." in: Advances in immunology, Vol. 31, pp. 177-245, (1982) (PubMed).
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: "Ligation of CD45 on B cells can facilitate production of secondary Ig isotypes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 3, pp. 1014-21, (1994) (PubMed).
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- CD45RA
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别名
- CD45RA
抗原
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