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CD45.1 抗体 (Biotin)

Name: CD45.1 抗体 (Biotin)
SKU: ABIN2689277
Availability: OutOfStock

This 小鼠单克隆 antibody specifically detects CD45.1 in FACS 和 IHC (fro). It exhibits reactivity toward 小鼠. It has been mentioned in 6+ publications

(6 references)

产品编号 ABIN2689277

发货至: 中国

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Quick Overview for CD45.1 抗体 (Biotin) (ABIN2689277)

抗原

CD45.1 (CD45 (CD45.1 Allogen))

适用

小鼠

宿主

小鼠

克隆类型

单克隆

标记

This CD45.1 antibody is conjugated to Biotin

应用范围

Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

A20

产品特性

The A20 antibody reacts with CD45 (Leukocyte Common Antigen) on all leukocytes of mouse strains expressing the CD45.1 alloantigen (eg, RIII, SJL/J, STS/A, DA). This alloantigen was originally named Ly-5.2, and this was the designation at the time that the antibody was characterized. The designation was later changed from Ly-5.2 to Ly-5.1 to conform with the convention that the .2 alloantigen designations be assigned to the C57BL/6 strain. mAb A20 has been reported not to react with leukocytes of most other mouse strains, which express the CD45.2 alloantigen. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus differing levels of glycosylation. The CD45 isoforms detected in the mouse are cell type-, maturation-, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction. The A20 antibody has been reported to inhibit some responses of B cells, from mice expressing the CD45.1 alloantigen, to antigens and LPS. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Differential expression of CD45.1 in RIII and C57BL/6 spleen. Single-cell suspensions of splenocytes from RIII (left panel) and C57BL/6N (right panel) mice were stained with biotin-conjugated A20 mAb followed by Avidin-FITC (Cat. No. 554057). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Biotin Mouse Anti-Mouse CD45.1 - Biotin - Clone A20 - Isotype Mouse IgG2a, κ - Reactivity Ms - 0.5 mg

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

SJL mouse thymocytes and splenocytes

亚型

IgG2a kappa
anti-CD45 (CD45.1 Allogen) antibody (Cy7,PE)
适用: 小鼠 FACS 宿主: 小鼠 Monoclonal A20 Cy7,PE

anti-CD45 (CD45.1 Allogen) antibody (PerCP-Cy5.5)
适用: 小鼠 FACS 宿主: 小鼠 Monoclonal A20 PerCP-Cy5.5

anti-CD45 (CD45.1 Allogen) antibody (violetFluor™ 450)
适用: 小鼠 FACS 宿主: 小鼠 Monoclonal A20 violetFluor™ 450

anti-CD45 (CD45.1 Allogen) antibody
适用: 小鼠 FACS 宿主: 小鼠 Monoclonal A20 unconjugated

anti-CD45 (CD45.1 Allogen) antibody (APC)
适用: 小鼠 FACS 宿主: 小鼠 Monoclonal A20 APC

anti-CD45 (CD45.1 Allogen) antibody (APC-Cy7)
适用: 小鼠 FACS 宿主: 小鼠 Monoclonal A20 APC-Cy7

anti-CD45 (CD45.1 Allogen) antibody (Biotin)
适用: 小鼠 FACS 宿主: 小鼠 Monoclonal A20 Biotin

anti-CD45 (CD45.1 Allogen) antibody (FITC)
适用: 小鼠 FACS 宿主: 小鼠 Monoclonal A20 FITC

anti-CD45 (CD45.1 Allogen) antibody (PE)
适用: 小鼠 FACS 宿主: 小鼠 Monoclonal A20 PE

anti-CD45 (CD45.1 Allogen) antibody (SPRD)
适用: 小鼠 FACS 宿主: 小鼠 Monoclonal A20 SPRD

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
浓度

0.5 mg/mL

缓冲液

Aqueous buffered solution containing ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

储存条件

4 °C

储存方法

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Suzuki, Oida, Hamada, Hitotsumatsu, Watanabe, Hibi, Yamamoto, Kubota, Kaminogawa, Ishikawa: "Gut cryptopatches: direct evidence of extrathymic anatomical sites for intestinal T lymphopoiesis." in: Immunity, Vol. 13, Issue 5, pp. 691-702, (2001) (PubMed).

Johnson, Greenbaum, Bottomly, Trowbridge: "Identification of the alternatively spliced exons of murine CD45 (T200) required for reactivity with B220 and other T200-restricted antibodies." in: The Journal of experimental medicine, Vol. 169, Issue 3, pp. 1179-84, (1989) (PubMed).

Morse, Shen, Hämmerling: "Genetic nomenclature for loci controlling mouse lymphocyte antigens." in: Immunogenetics, Vol. 25, Issue 2, pp. 71-8, (1987) (PubMed).

Shen, Tung, Boyse: "Further definition of the Ly-5 system." in: Immunogenetics, Vol. 24, Issue 3, pp. 146-9, (1986) (PubMed).

Yakura, Kawabata, Shen, Katagiri: "Selective inhibition of lipopolysaccharide-induced polyclonal IgG response by monoclonal Ly-5 antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 136, Issue 8, pp. 2729-33, (1986) (PubMed).

Yakura, Shen, Bourcet, Boyse: "On the function of Ly-5 in the regulation of antigen-driven B cell differentiation. Comparison and contrast with Lyb-2." in: The Journal of experimental medicine, Vol. 157, Issue 4, pp. 1077-88, (1983) (PubMed).
抗原

CD45.1 (CD45 (CD45.1 Allogen))

别名

CD45.1