CD3 抗体
(3 references)Our Local Distributor
北京 101111
Quick Overview for CD3 抗体 (ABIN2689164)
抗原
See all CD3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
产品特性
-
The 17A2 antibody reacts with the T-cell receptor-associated CD3 complex, which is expressed on many thymocytes and mature T lymphocytes. Plate-bound mAb 17A2 has been reported to induce IL-2 production by T cells in the absence of accessory cells. Binding of 17A2 antibody to T cells can be blocked by the anti-CD3e mAb 145-2C11, suggesting that 17A2 antibody recognizes an epitope of the CD3 epsilon chain. In vivo treatment with 17A2 mAb has been reported to partially deplete T lymphocytes and temporarily down-modulates CD3 expression on T cells. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. CD3 expression in spleen and thymus. BALB/c splenocytes were simultaneously stained with purified mAb 17A2 (bottom left panel) and PE-conjugated anti-mouse CD19 mAb 1D3 (top left & bottom left panels), followed by FITC-conjugated anti-rat IgG2b mAb G15-337 (Cat. No. 553884) (top left & bottom left panels). BALB/c thymocytes were also stained with 17A2 mAb (bottom right panel), followed by FITC-conjugated anti-rat IgG2b (top right & bottom right panels). Flow cytometry was performed on a BD FACSCan™ flow cytometry system.
BD Pharmingen™ Purified Rat Anti-Mouse CD3 Molecular Complex - Purified - Clone 17A2 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.5 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
-
免疫原
- gammadelta TCR-positive T-T hybridoma D1
-
亚型
- IgG2b kappa
-
-
anti-CD3 (CD3) antibody
CD3 适用: 人, 小鼠, 大鼠 IHC, IF, WB 宿主: 兔 Polyclonal unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro), ICC, MS 宿主: 小鼠 Monoclonal UCHT1 unconjugated
anti-CD3 (CD3) antibody (APC)CD3 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-57 APC
anti-CD3 (CD3) antibody (Biotin)CD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro) 宿主: 小鼠 Monoclonal UCHT1 Biotin
anti-CD3 (CD3) antibody (PerCP)CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 PerCP
anti-CD3 (CD3) antibody (APC)CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 APC
anti-CD3 (CD3) antibodyCD3 适用: 人 FACS, IP, Func, MS 宿主: 小鼠 Monoclonal MEM-57 unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro), Func, MS 宿主: 小鼠 Monoclonal UCHT1 unconjugated
anti-CD3 (CD3) antibody (PerCP)CD3 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-57 PerCP
anti-CD3 (CD3) antibody (PE)CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 PE
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
限制
- 仅限研究用
-
-
-
浓度
- 0.5 mg/mL
-
缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C
-
储存方法
- Store undiluted at 4°C.
-
-
-
: "Antilymphocytic antibodies and marrow transplantation. XII. Suppression of graft-versus-host disease by T-cell-modulating and depleting antimouse CD3 antibody is most effective when preinjected in ..." in: Blood, Vol. 80, Issue 10, pp. 2661-7, (1992) (PubMed).
: "Developmental potential of the earliest precursor cells from the adult mouse thymus." in: The Journal of experimental medicine, Vol. 174, Issue 6, pp. 1617-27, (1992) (PubMed).
: "Production and characterization of a rat monoclonal antibody against the murine CD3 molecular complex." in: Immunology letters, Vol. 23, Issue 2, pp. 113-8, (1990) (PubMed).
-
-
- CD3
-
别名
- CD3
-
途径
- TCR Signaling, Ubiquitin Proteasome Pathway
抗原
-
