CD3 抗体
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北京 101111
Quick Overview for CD3 抗体 (ABIN2689163)
抗原
See all CD3 抗体适用
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应用范围
克隆位点
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产品特性
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The 1F4 antibody reacts with the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes, peripheral T lymphocytes, and dendritic epidermal T cells. In vitro, immobilized 1F4 mAb induces T-cell proliferation, and soluble mAb synergizes with PMA for T-cell activation. Pre-incubation of splenocytes with mAb 1F4 blocks staining with mAb G4.18 (Cat. No. 559974/554830). This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. CD3e expression in spleen and thymus. LEW splenocytes were preincubated with Rat BD Fc Block™ purified anti-rat CD32 mAb D34-485 (Cat. No. 550270/550271) and simultaneously stained with FITC-onjugated anti-rat CD5 mAb OX-19 (Cat. No. 551449, left panels) and purified mAb 1F4 (middle left panel), followed by PE-conjugated anti-mouse IgMa mAb DS-1 (Cat. No. 553517, left panels). LEW thymocytes were stained with purified 1F4 mAb (far right panel) followed by PE-conjugated anti-mouse IgMa mAb DS-1 (right panels). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
BD Pharmingen™ Purified Mouse Anti-Rat CD3 - Purified - Clone 1F4 - Isotype Mouse IgM, κ - Reactivity Rat - 0.5 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- F344 rat splenic T cells stimulated with PMA plus A23187
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亚型
- IgM kappa
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anti-CD3 (CD3) antibody
CD3 适用: 人, 小鼠, 大鼠 IHC, IF, WB 宿主: 兔 Polyclonal unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro), ICC, MS 宿主: 小鼠 Monoclonal UCHT1 unconjugated
anti-CD3 (CD3) antibody (APC)CD3 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-57 APC
anti-CD3 (CD3) antibody (Biotin)CD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro) 宿主: 小鼠 Monoclonal UCHT1 Biotin
anti-CD3 (CD3) antibody (PerCP)CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 PerCP
anti-CD3 (CD3) antibody (APC)CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 APC
anti-CD3 (CD3) antibodyCD3 适用: 人 FACS, IP, Func, MS 宿主: 小鼠 Monoclonal MEM-57 unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro), Func, MS 宿主: 小鼠 Monoclonal UCHT1 unconjugated
anti-CD3 (CD3) antibody (PerCP)CD3 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-57 PerCP
anti-CD3 (CD3) antibody (PE)CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 PE
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Alloreactive natural killer cells in the rat: complex genetics of major histocompatibility complex control." in: European journal of immunology, Vol. 25, Issue 5, pp. 1249-56, (1995) (PubMed).
: "T-cell receptor diversity in dendritic epidermal T cells in the rat." in: The Journal of investigative dermatology, Vol. 102, Issue 1, pp. 74-9, (1994) (PubMed).
: "Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody." in: Transplantation, Vol. 55, Issue 3, pp. 459-68, (1993) (PubMed).
: "Characterization of a CD3-like rat T cell surface antigen recognized by a monoclonal antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 8, pp. 2791-5, (1989) (PubMed).
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- CD3
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别名
- CD3
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途径
- TCR Signaling, Ubiquitin Proteasome Pathway
抗原
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