ITGB1 抗体
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Quick Overview for ITGB1 抗体 (ABIN2689158)
抗原
See all ITGB1 抗体适用
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应用范围
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产品特性
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The NaM160-1A3 antibody reacts with the 116- kDa integrin β1 chain (CD29). CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin α chains. With α1 through α6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with αv (CD51), it forms αvβ1 integrin. As a result, CD29 has a broad tissue distribution, including leukocytes, endothelia, epithelia, and oocytes. Porcine CD29 is believed to be a major target for natural antibodies involved in rejection of pig-to-human xenografts, and a mAb to block recognition of pig CD29 may have therapeutic applications. NaM160-1A3 mAb does not cross-react with human peripheral blood lymphocytes or umbilical cord vein endothelial cells. Expression of CD29 on pig peripheral blood leukocytes. Pig whole blood was stained with either purified mAb NaM160-1A3 (filled histograms) or purified mouse IgG1, κ isotype control mAb MOPC-31C (Cat. No. 557273, open histograms), followed by FITC-conjugated goat anti-mouse Ig (multiple adsorption, Cat. No. 554001). Erythrocytes were lysed (PharM Lyse™, Cat. No. 555899), non-viable leukocytes were excluded by staining with 7-AAD (Via-Probe™, Cat. No. 555816/555815), and lymphocytes (left panel), monocytes (center panel), and granulocytes (right panel) were gated according to their light-scatter profiles. Multi-color staining (data not shown) demonstrates that most CD4-CD8+ T and NK cells and most CD4+CD8+ memory T cells make up the CD29bright lymphocyte population, CD4+CD8- T lymphocytes are CD29dim, and the CD4-CD8- lymphocytes have CD29negative and CD29dim subpopulations. Flow cytometry was performed on a FACSCalibur™ .
BD Pharmingen™ Purified Mouse Anti-Pig CD29 - Purified - Clone NaM160-1A3 - Isotype Mouse IgG1, κ - Reactivity Pig - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Pig platelets
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亚型
- IgG1 kappa
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anti-Integrin beta 1 (ITGB1) (AA 25-100) antibody
ITGB1 适用: 人, 小鼠, 大鼠, 绵羊, 兔 WB, FACS, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Integrin beta 1 (ITGB1) antibodyITGB1 适用: 人, 猴 WB, FACS, ELISA, IHC 宿主: 小鼠 Monoclonal 3B6 unconjugated
anti-Integrin beta 1 (ITGB1) (AA 140-383) antibodyITGB1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Integrin beta 1 (ITGB1) antibodyITGB1 适用: 人, Pig, 犬 WB, FACS, IP 宿主: 小鼠 Monoclonal MEM-101A unconjugated
anti-Integrin beta 1 (ITGB1) antibody (PE)ITGB1 适用: 人, Pig, 犬 FACS 宿主: 小鼠 Monoclonal MEM-101A PE
anti-Integrin beta 1 (ITGB1) antibodyITGB1 适用: 人 WB, FACS, ELISA, IHC, IF 宿主: 兔 Monoclonal 32A10 unconjugated Recombinant Antibody
anti-Integrin beta 1 (ITGB1) antibodyKD Validated ITGB1 适用: 人 WB, FACS, IF (p) 宿主: 兔 Monoclonal 23GB885 unconjugated Recombinant Antibody
anti-Integrin beta 1 (ITGB1) antibodyKD Validated ITGB1 适用: 人 WB, FACS, IF (p) 宿主: 兔 Monoclonal 23GB890 unconjugated Recombinant Antibody
anti-Integrin beta 1 (ITGB1) (AA 454-714) antibodyITGB1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Integrin beta 1 (ITGB1) (AA 140-383) antibodyITGB1 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C1 unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Molecular cloning and characterization of the pig homologue to human CD29, the integrin beta1 subunit." in: Transplantation, Vol. 70, Issue 4, pp. 649-55, (2000) (PubMed).
: "Potential role of alphav and beta1 integrins as oocyte adhesion molecules during fertilization in pigs." in: Journal of reproduction and fertility, Vol. 120, Issue 1, pp. 65-72, (2000) (PubMed).
: "Characterization of a murine monoclonal antibody specific for swine beta1 integrin." in: Xenotransplantation, Vol. 5, Issue 1, pp. 75-83, (1998) (PubMed).
: "Adhesion receptors of the immune system." in: Nature, Vol. 346, Issue 6283, pp. 425-34, (1990) (PubMed).
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: "Molecular cloning and characterization of the pig homologue to human CD29, the integrin beta1 subunit." in: Transplantation, Vol. 70, Issue 4, pp. 649-55, (2000) (PubMed).
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- ITGB1 (Integrin beta 1 (ITGB1))
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别名
- CD29
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背景
- Synonyms: Integrin β1 chain
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途径
- Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome
抗原
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