TLR4 /MD2 Complex 抗体
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北京 101111
Quick Overview for TLR4 /MD2 Complex 抗体 (ABIN2689150)
抗原
适用
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克隆类型
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应用范围
克隆位点
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产品特性
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The MTS510 antibody reacts with the molecular complex of Toll-Like Receptor 4 and MD-2 (TLR4-MD-2) which is expressed on LPS responsive macrophages. TLR4, a member of the Toll-Like Receptor Family, has been renamed CD284 and identified to be the transmembrane signal-transducing portion of the receptor for LPS. TLR4 associates on the cell surface with CD14 and MD-2, a 0.7 kDa molecule which is anchored to the membrane via its physical association with TLR4. The association of MD-2 with TLR4 is required for recognition of LPS and the anti-mitotic compound Taxol, which mimics the action of LPS on mouse cells. MTS510 mAb detects TLR4-MD-2 on the surface of thioglycollate-elicited macrophages from all mouse strains tested (ie, BALB/c, C57BL/6, C3H/HeJ, C3H/HeN, and DBA/1), including the C3H/HeJ strain which expresses an LPS-resistant mutant TLR4. Expression of TLR4-MD-2 is down-regulated on peritoneal macrophages after exposure to LPS, correlating with the occurrence of LPS tolerance. TLR4-MD-2 is not detected on splenocytes or thymocytes. Expression of TLR4-MD-2 on peritoneal macrophages. Thioglycollate-elicited peritoneal macrophages from BALB/c mice were stained with either purified rat IgG2a, κ isotype control mAb R35-95 (Cat. No. 553927, left panel) or purified mAb MTS510 (right panel), in the presence of Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. No. 553141/553142). Staining was detected by biotinylated anti-rat IgG2a mAb RG7/1.30 (Cat. No. 553894) then Streptavidin-PE (Cat. No. 554061). The macrophages were identified by staining with FITC-conjugated anti-mouse CD11b (Integrin αM chain) mAb M1/70 (Cat. No. 557396/553310). The total viable leukocytes are displayed. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
BD Pharmingen™ Purified Rat Anti-Mouse CD284/MD-2 Complex - Purified - Clone MTS510 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Mouse Pro-B cell line Ba/F3 expressing transfected BALB/c mouse TLR4 and MD-2
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亚型
- IgG2a kappa
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anti-TLR4 /MD2 Complex antibody (PE)
适用: 小鼠 FACS 宿主: 大鼠 Monoclonal MTS510 PE
anti-TLR4 /MD2 Complex antibody适用: 小鼠 FACS, IP, IHC, IHC (fro) 宿主: 大鼠 Monoclonal MTS510 unconjugated
anti-TLR4 /MD2 Complex antibody (Cy7,PE)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal MTS510 Cy7,PE
anti-TLR4 /MD2 Complex antibody (APC)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal MTS510 APC
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Lipopolysaccharide interaction with cell surface Toll-like receptor 4-MD-2: higher affinity than that with MD-2 or CD14." in: The Journal of experimental medicine, Vol. 198, Issue 7, pp. 1035-42, (2003) (PubMed).
: "The sole gateway to endotoxin response: how LPS was identified as Tlr4, and its role in innate immunity." in: Drug metabolism and disposition: the biological fate of chemicals, Vol. 29, Issue 4 Pt 2, pp. 474-8, (2001) (PubMed).
: "Cutting edge: cell surface expression and lipopolysaccharide signaling via the toll-like receptor 4-MD-2 complex on mouse peritoneal macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 7, pp. 3471-5, (2000) (PubMed).
: "Cutting edge: endotoxin tolerance in mouse peritoneal macrophages correlates with down-regulation of surface toll-like receptor 4 expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 7, pp. 3476-9, (2000) (PubMed).
: "MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1777-82, (1999) (PubMed).
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: "Lipopolysaccharide interaction with cell surface Toll-like receptor 4-MD-2: higher affinity than that with MD-2 or CD14." in: The Journal of experimental medicine, Vol. 198, Issue 7, pp. 1035-42, (2003) (PubMed).
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- TLR4 /MD2 Complex
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别名
- CD284/MD-2 Complex
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背景
- Synonyms: TLR4/MD-2 Complex
抗原
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